July 23rd 2024
At HPLC 2024 in Denver, Colorado, various sessions will be led by experienced scientists, covering different aspects of chromatography techniques and maximizing their effectiveness. Here is some information about tomorrow’s sessions.
Rising Stars of Separation Science: Zhuoheng Zhou
December 5th 2023This month we spoke to Zhuoheng Zhou from the Vrije Universiteit Brussel in Belgium about his research on advancing instrumental and column technologies for modern chromatography and its applications in novel biopharmaceutical modalities and proteomics.
UHPLC–MS Method Used to Detect Purine Metabolites and Study Severe Biological Disorders
December 5th 2023A recent study published in the Journal of Separation Science reveals how ultrahigh-performance liquid chromatography tandem mass spectrometry (UHPLC–MS/MS) could be used to detect and quantify purine metabolites.
Why is Open-Access Hyphenation Important for Chromatographers?
October 4th 2023At HPLC 2023, John Langley, a professor at the University of Southampton in Southampton, UK, presented a talk called "(R)evolution of Hyphenations, Through Open-Access RP-HPLC–MS, Open-Access UHPSFC–MS and 2D-LC–IMS-MS in Academia". He spoke with LCGC on the importance of open-access hyphenation, and how to implement it in your research.
Scientists used supercritical fluid chromatography (SFC) and ultrahigh-pressure liquid chromatography (UHPLC)—both coupled with high-resolution mass spectrometry (HRMS)—to analyze various types of chlorinated paraffins (CPs) in fish oil-based dietary supplements.
Enhancing Peptide Mapping Sequence Coverage Through an Automated Dual Protease Digest
August 1st 2023Trypsin is one of the most commonly used proteases in peptide mapping protocols because of its high level of specificity. However, trypsin alone is not always sufficient for full sequence coverage. In this article, the authors detail how trypsin was combined with chymotrypsin to overcome this, and the benefits of an automated platform.
CIL-UHPLC–HRMS Profiles Carbonyl Compounds in Baijiu Daqu Samples
July 27th 2023Sauce-flavor or sauce-aroma baijiu, one variant of a popular Chinese liquor, is treated with a fermentation starter called daqu, giving the concoction a unique taste profile, and scientists want more information about how it can inform the manufacturing of higher-quality baijiu.
LC×LC With UV Detection and 3D Contour Plot Differentiates Ginseng in Two Types of Chinese Medicine
July 13th 2023Authentication of popular ginseng-based products has been difficult and has caused confusion in quality control between traditional Chinese medicine (TCM) and Chinese patent medicine (CPM).
Successful Analysis of Protein Biopharmaceuticals Using Ultra-Short Columns
June 20th 2023Davy Guillarme of the University of Geneva said in a talk at HPLC 2023 that short columns have shown promise, but ultrahigh-performance liquid chromatography (UHPLC) instrumentation is not yet optimized to realize their benefits.
UHPLC–MS/MS Quantifies Ultraviolet Filter Compounds in Chlorinated Canadian Waters
June 16th 2023An ultrahigh-pressure liquid chromatography–tandem mass spectrometry method (UHPLC–MS/MS) using polarity switching, preceded by solid-phase extraction (SPE), was used to quantify eight ultraviolet filter (UVF) compounds in chlorinated outdoor pools in Winnipeg.