Application Notes: LC

PharmaFluidics introduces a new μPAC capLC column that offers unprecedented performance and that given its low backpressure can be used with flow rates of up to 15μL/min.

PharmaFluidics also offers a 50 cm long μPAC column which is perfectly suited to perform more throughput analyses with shorter gradient solvent times.

Analyzing skin ceramides using PharmaFluidics’ µPAC column technology reveals a substantial structural diversity and isomeric resolved sphingolipids.

Monitoring the excellent separation performance of PharmaFluidics’ µPAC columns by using peptide retention time calibration mixtures for bottom-up proteomics.

PharmaFluidics’ μPAC columns have the potential to be less prone to sample related column failure and to withstand more sample injections without losing performance.

Today’s conference highlights

Click the title above to open the LCGC Europe June 2020 regular issue, Volume 33, Number 6, in an interactive PDF format.

Dow scientists improved their method-development workflow by upgrading their hardware and using specialized software.

PharmaFluidics’ μPAC™ capLC is evaluated for different user needs ranging from 12 samples per day in comprehensive mode to 65 samples per day in throughput mode.

YMC’s latest HIC column is used to analyse the DAR of Brentuximab vedotin allowing higher flow rates and shorter run times thanks to the pressure resistant particle.

The dedicated SEC column for antibodies, YMC-SEC MAB, is used to separate the MAb monomer from its aggregates as well as from degradation products in a single run.

The less hydrophobic wide-pore column YMC-Triart Bio C4 is used for the analysis of modified oligonucleotides due to the hydrophobic character of the disulfide units.

YMC-Triart Bio C4 is used in YMC capillary column format to analyse and identify intact commercial MAbs by micro-LC- MS/MS at an elevated temperature of 75°C.

This application note shows how to achieve narrow peaks and high sensitivity by optimising the AEX separation of phosphorothioate oligonucleotides (PS) in only 5 steps.

In this application note eight intact proteins in the protein mix MSRT2 are analysed by Micro-LC–MS using an YMC-Triart Bio C4 capillary column.

This application note using YMC-Triart Bio C4 shows a robust separation at high temperatures of 3 commercially available MAbs: Ofatumumab, Palivizumab, and Panitumumab.

In this application YMC-Triart Bio C4 was used to analyse a reduced monoclonal antibody and a reduced ADC mimic with formic acid or TFA as additives.

“GC Connections” presents the column’s annual review of new developments in the field of gas chromatography seen at Pittcon and other venues in the past 12 months.

An HCP analysis workflow including the Agilent AssayMAP Bravo platform for automated sample preparation, 6545XT AdvanceBio LC/Q‑TOF for LC-MS/MS analysis

This whitepaper demonstrates the features and benefits of owning an Ultivo LC/TQ in terms of actual tangible benefits to your laboratory operations.

A method describing the detection of 12 potentially mutagenic nitrosamine impurities found in sartan drugs using the Ultivo LC/TQ

Use interactive calculators included in this whitepaper to determine the incremental economic value of the Agilent 1260 Infinity II Prime LC and InfinityLab LC/MSD iQ.

This application note investigates the durability of YMC’s high-strength “HG” Cyano phase under highly acidic and highly basic mobile phase conditions.

This application note details a loading study for purifying cannabidiol (CBD) from a commercially available “high-CBD” hemp oil extract.

This application note details improved HPLC assay methods for chlorhexidine gluconate on hybrid-silica and core-shell C18 stationary phases.