Application Notes: LC

Robust and accurate primary sequence confirmation and monitoring of post-translational modifications of the SARS-CoV-2 spike protein using the BioAccord LC–MS system.

Eliminate the need for column conditioning, improve recovery, and improve repeatability by minimizing non-specific oligonucleotide interactions with metal surfaces.

A rapid, sensitive, and reproducible method for understanding the SARS-CoV-2 spike protein N-glycosylation profile using the GlycoWorks RapiFluor MS N-Glycan Kit.

Review of the best practices for oligonucleotide analysis and purification to achieve high quality oligonucleotides for COVID-19 diagnostic or therapeutic applications.

A HILIC method for baseline separation of ribavirin from isobaric and structurally similar species within 6 minutes using an ACQUITY UPLC BEH Amide column.

This work demonstrates how to achieve sharper peaks and faster elution of basic analytes like umifenovir using a mixed-mode Atlantis PREMIER BEH C18 AX column.

We discuss the role of LC and LC–MS for select COVID-19 applications to better understand the use of analytical technologies mobilized in the fight against the pandemic.

This white paper demonstrates a vendor’s perspective on recent trends in regulations and the potential future direction for the industry.

This application note demonstrates the direct analysis of biogenic amines in Tempranillo red wine using ion pair chromatography prior to MS analysis.

This white paper demonstrates a vendor’s perspective on recent trends in regulations and the potential future direction for the industry.

The baseline separation of various chlorophenols, that only differ in the amount and/or position of the chlorine substituents, is made possible using YMC-Triart C18 ExRS.

Modified nucleosides, either natural or synthetic, often provide RNA with distinctive properties useful in diagnostics or therapeutics.  

Modified nucleosides, either natural or synthetic, often provide RNA with distinctive properties useful in diagnostics or therapeutics.

Retention under reverse-phase conditions as a consequence of polar end capping on stationary phase provides sites for interaction with the polar entities within Warfarin.

This application illustrates the efficient separation of three sulfa drugs using a Quasar C18 HPLC column.

The Quasar C18 HPLC phase offers high efficiency separation of the semi-synthetic antibiotic, amoxicillin.

We present retention-time models for peptides and proteins that link their elution times to solvent fraction and temperature.

Learn how impurity-monitoring software can improve your understanding of your process route and help you track fate and purge.  

Adsorption of metal-sensitive analytes in LC-based assays can negatively impact data. Learn how to minimize analyte–surface interaction and improve reproducibility.

We discuss the theory, principles, and key equations required for method translation for both isocratic and gradient LC methods using the Avantor® ACE® Method Translator tool.

Elution profiles based on mAb glycan structures produced by the TSKgel® FcR-IIIA-NPR™ affinity chromatography column correlate with results from HILIC glycan profiling.  

Highlighting the use of the dial-a-mix method for screening different mobile-phase pH conditions during method development of IEX charge variant analysis.

This application note details the development of a HPLC method for analyzing CBD and Melatonin in an over-the-counter sleep aid.

This application note highlights the ability of YMC-Triart C18 to separate 11 common cannabinoids encountered during potency testing.

A loading study for purifying cannabidiol (CBD) from a commercially available “high-CBD” hemp oil extract

Demonstrating the use of YMC’s new 2µm Diol stationary phase for scaling down size exclusion analyses

Here we present an innovative approach for the characterization of nanoplastic particles using centrifugal field-flow fractionation coupled with UV, multi angle light Scattering detection, and raman microscopy.

The Torion® T-9 GC/MS system has characterized several high boiling point organochlorine pesticides in tea samples in less than 10 minutes.