Application Notes: LC

Adsorption of metal-sensitive analytes in LC-based assays can negatively impact data. Learn how to minimize analyte–surface interaction and improve reproducibility.

We discuss the theory, principles, and key equations required for method translation for both isocratic and gradient LC methods using the Avantor® ACE® Method Translator tool.

Elution profiles based on mAb glycan structures produced by the TSKgel® FcR-IIIA-NPR™ affinity chromatography column correlate with results from HILIC glycan profiling.  

Highlighting the use of the dial-a-mix method for screening different mobile-phase pH conditions during method development of IEX charge variant analysis.

This application note details the development of a HPLC method for analyzing CBD and Melatonin in an over-the-counter sleep aid.

This application note highlights the ability of YMC-Triart C18 to separate 11 common cannabinoids encountered during potency testing.

A loading study for purifying cannabidiol (CBD) from a commercially available “high-CBD” hemp oil extract

Demonstrating the use of YMC’s new 2µm Diol stationary phase for scaling down size exclusion analyses

Here we present an innovative approach for the characterization of nanoplastic particles using centrifugal field-flow fractionation coupled with UV, multi angle light Scattering detection, and raman microscopy.

This application note discusses using a Quasar™ C18 column to analyze azithromycin, an antibiotic used to treat various bacterial infections.

SPME-UHPLC/MS/MS offers an efficient alternative for the analysis of veterinary drug residues in animal tissue as compared to traditional sampling procedures.

The Quasar biphenyl HPLC phase offers high efficiency separation and excellent peak shape of the synthetic antibiotic, ciprofloxacin.

This application note describes using a Quasar biphenyl LC column in analyzing several cephalosporins, a mixture of first and second generation β-lactam antibiotics.

A highly sensitive, validated method for atrazine determination in different water types using the QSight 420 LC–MS/MS employed a simple filtration and direct injection.

Using the LX50 UHPLC and QSight 420 triple quad MS, three analytical methods were developed to provide sensitive, reproducible results without the need for pre-concentration steps.

A methodology for a sensitive and repeatable analysis of PPCPs in drinking water utilizing the QSight SP50 Online SPE system coupled to a QSight 220 MS/MS.

IdeS, a cysteine protease, is used for partial digestion of mAbs and generates a (Fab’)2 fragment and two Fc fragments (Fc/2) for better characterization of the Fc fragment.

Selectivity of two high-pH stable Agilent InfinityLab Poroshell 120 C18 columns is compared with pesticides using a pH 10 formate buffer and acetonitrile gradient.

This application combines cysteine proteases IgdE and IdeS, to observe conserved and hypervariable regions of the antibody, as well as identification of other variants.

IgdE allows for better characterization of the antibody and yields an intact Fc and an intact Fab fragment that can be further isolated and characterized.

Comparing core-shell superficially porous to fully porous particles to determine efficiency required for detection, separation of low-level impurities for subunit analysis.

A reversed-phase method using a superficially porous, wide pore particle morphology for the quantitation of coformulated mAbs leads to high efficiency separations.

For LC–MS analysis of NIST mAb, multiple C4 LC columns are compared to determine which provides necessary separation of impurity and avoids spectral overlap in the MS detector.

The performance of basic pharmaceuticals is compared to the charged surface Agilent InfinityLab Poroshell 120 CS-C18 and a C18 with formic acid mobile phase.

For Fc Fusion proteins a reversed-phase LC analytical method is used for either extended characterization and identification for impurities or as a simple purity method.

Loading capacity and peak shape of basic pharmaceuticals were compared on the Agilent InfinityLab Poroshell 120 CS-C18 and a traditional C18 column with formic acid.

Intact reversed-phase LC method is implemented for a simple yet high-resolution analytical technique that can be used for the purity analysis of monoclonal antibodies.

This white paper provides a vendor’s perspective on recent trends in regulations and the potential future direction for the industry referred to as “Pharma 4.0”.