Application Notes: LC

The value of a wide range of pH compatibility is explored. A pesticide mixture shows how eluent pH affects analyte retention for acids, bases, and neutrals.

The unique selectivity of four Agilent InfinityLab Poroshell 120 C18 columns is shown with veterinary drugs using a simple formic acid and acetonitrile gradient.

Using Empower CDS and NuGenesis LMS to develop an archive strategy that reduces backup and recovery times and removes manual intervention is demonstrated.

Companies can seek outside help for backup and restore, disaster recovery, and system administration, but responsibility for regulated data remains with the company.

VITAQUEST, a contract manufacturer of dietary supplements, used NuGenesis LMS to automate its workflows, improve lab efficiency, and reduce the risk for data errors.

This study shows how coupling Waters’ Empower CDS with cloud technology allowed a pharmaceutical company to streamline its global operations and connect each of its labs.

This white paper explains the 21 CFR Part 11 and EU EudraLex Annex 11 compliance readiness of Waters Empower Software for the regulated scientific laboratory.

This case study demonstrates the advantages of pairing Empower CDS with NuGenesis LMS to improve the availability of your CDS data and maximize its effectiveness.

A&M Stabtest, an independent CRO in the pharmaceutical industry, reduced time-consuming and error-prone manual steps in the chromatographic testing process.

This study demonstrates Waters ACQUITY UPLC with PDA/QDa technology and Empower Software were integral to successful method lifecycle management at Hovione, a global CDMO.

Companies can reduce the fallout of a disaster via a business continuity plan with appropriate hardware and software, including Waters Empower BC LAC/E with SecureSync.

Accurate detection of HAA’s is paramount for the prevention of over oxidation, while still managing enough water sanitation to eliminate water borne pathogens.

YMC’s latest HIC column is used to analyse the DAR of Brentuximab Vedotin allowing higher flow rates and shorter run times thanks to the pressure resistant particle.

YMC’s HIC columns, BioPro HIC HT, and BioPro HIC BF address separation requirements of different compounds. The separation behaviour of two mAbs is demonstrated.

This application note shows how a mixture of seven in-house mAbs, as well as the molecular variants of two mAbs, were characterised via HIC-MS using YMC’s BioPro HIC BF column.

In this application note the DAR of the SigmaMAb ADC-mimic was determined using YMC’s BioPro HIC BF column and a nanospray ionisation mass spectrometer.

Monoclonal antibodies and their oxidised species are separated using YMC’s more hydrophobic BioPro HIC BF column, dedicated for low hydrophobicity proteins and oxidised mAb variants.

Four therapeutic mAbs of different species, isotypes, and pI together with their variants were analysed using pH and salt gradient mode using YMC’s non-porous BioPro IEX SF.

YMC-Triart Bio C4 is used in YMC capillary column format to analyse and identify intact commercial mAbs by micro LC–MS/MS at an elevated temperature of 75°C.

In this application note high-resolution SCX was coupled directly to an ultrasensitive online native MS using YMC’s BioPro IEX SF to analyse mAb charge variants.

The use of a C18 column for the analysis of indoxyl sulfate as part of a research study to measure the total levels in a simulated blood serum environment is illustrated.

Discover Coiled microextraction and SPME field collection and injection devices using portable GC–MS for separation, detection, and identification of explosive materials.

Vitamin C is essential for the repair of tissue and is thought to lower cancer risk. This application brief describes use of a Quasar biphenyl column in this analysis.

Plavix tablets contain the active ingredient clopidogrel hydrogen sulphate, readily analyzed by HPLC. Use of a Quasar C8 column in the analysis of Plavix is described.

Detect whisky and spirits adulteration. Field-portable GC–MS provides rapid determination of denatured alcohol when added to spirit drinks to raise alcohol content.

PharmaFluidics’ superior fabrication robustness of microfabricated LC columns results in sub-second retention time variation over three different μPAC™ capLC columns.

A CDS implemented appropriately and managed correctly can simplify system administration, ensure regulatory compliance and adherence to data integrity guidelines

Software solutions can guide all stages of pharmaceutical stress testing, from prediction of degradation chemistry to analytical development and, project management.