Application Notes: LC

The Quasar biphenyl HPLC phase offers high efficiency separation and excellent peak shape of the synthetic antibiotic, ciprofloxacin.

This application note describes using a Quasar biphenyl LC column in analyzing several cephalosporins, a mixture of first and second generation β-lactam antibiotics.

A highly sensitive, validated method for atrazine determination in different water types using the QSight 420 LC–MS/MS employed a simple filtration and direct injection.

Using the LX50 UHPLC and QSight 420 triple quad MS, three analytical methods were developed to provide sensitive, reproducible results without the need for pre-concentration steps.

A methodology for a sensitive and repeatable analysis of PPCPs in drinking water utilizing the QSight SP50 Online SPE system coupled to a QSight 220 MS/MS.

IdeS, a cysteine protease, is used for partial digestion of mAbs and generates a (Fab’)2 fragment and two Fc fragments (Fc/2) for better characterization of the Fc fragment.

Selectivity of two high-pH stable Agilent InfinityLab Poroshell 120 C18 columns is compared with pesticides using a pH 10 formate buffer and acetonitrile gradient.

This application combines cysteine proteases IgdE and IdeS, to observe conserved and hypervariable regions of the antibody, as well as identification of other variants.

IgdE allows for better characterization of the antibody and yields an intact Fc and an intact Fab fragment that can be further isolated and characterized.

A reversed-phase method using a superficially porous, wide pore particle morphology for the quantitation of coformulated mAbs leads to high efficiency separations.

For LC–MS analysis of NIST mAb, multiple C4 LC columns are compared to determine which provides necessary separation of impurity and avoids spectral overlap in the MS detector.

The performance of basic pharmaceuticals is compared to the charged surface Agilent InfinityLab Poroshell 120 CS-C18 and a C18 with formic acid mobile phase.

For Fc Fusion proteins a reversed-phase LC analytical method is used for either extended characterization and identification for impurities or as a simple purity method.

Loading capacity and peak shape of basic pharmaceuticals were compared on the Agilent InfinityLab Poroshell 120 CS-C18 and a traditional C18 column with formic acid.

Intact reversed-phase LC method is implemented for a simple yet high-resolution analytical technique that can be used for the purity analysis of monoclonal antibodies.

This white paper provides a vendor’s perspective on recent trends in regulations and the potential future direction for the industry referred to as “Pharma 4.0”.

Analyzing diverse method parameters, such as temperature, gradient program, and flow rates for optimization of intact mass applications when using bioZen WidePore C4.

The value of a wide range of pH compatibility is explored. A pesticide mixture shows how eluent pH affects analyte retention for acids, bases, and neutrals.

The unique selectivity of four Agilent InfinityLab Poroshell 120 C18 columns is shown with veterinary drugs using a simple formic acid and acetonitrile gradient.

Using Empower CDS and NuGenesis LMS to develop an archive strategy that reduces backup and recovery times and removes manual intervention is demonstrated.

Companies can seek outside help for backup and restore, disaster recovery, and system administration, but responsibility for regulated data remains with the company.

VITAQUEST, a contract manufacturer of dietary supplements, used NuGenesis LMS to automate its workflows, improve lab efficiency, and reduce the risk for data errors.

This study shows how coupling Waters’ Empower CDS with cloud technology allowed a pharmaceutical company to streamline its global operations and connect each of its labs.

This white paper explains the 21 CFR Part 11 and EU EudraLex Annex 11 compliance readiness of Waters Empower Software for the regulated scientific laboratory.

This case study demonstrates the advantages of pairing Empower CDS with NuGenesis LMS to improve the availability of your CDS data and maximize its effectiveness.

A&M Stabtest, an independent CRO in the pharmaceutical industry, reduced time-consuming and error-prone manual steps in the chromatographic testing process.

An investment in Waters NuGenesis LMS helped a global pharmaceutical CDMO meet demands for data integrity regulatory compliance and laboratory management needs.

This study demonstrates Waters ACQUITY UPLC with PDA/QDa technology and Empower Software were integral to successful method lifecycle management at Hovione, a global CDMO.