Application Notes: LC

Accurate detection of HAA’s is paramount for the prevention of over oxidation, while still managing enough water sanitation to eliminate water borne pathogens.

YMC’s latest HIC column is used to analyse the DAR of Brentuximab Vedotin allowing higher flow rates and shorter run times thanks to the pressure resistant particle.

YMC’s HIC columns, BioPro HIC HT, and BioPro HIC BF address separation requirements of different compounds. The separation behaviour of two mAbs is demonstrated.

This application note shows how a mixture of seven in-house mAbs, as well as the molecular variants of two mAbs, were characterised via HIC-MS using YMC’s BioPro HIC BF column.

In this application note the DAR of the SigmaMAb ADC-mimic was determined using YMC’s BioPro HIC BF column and a nanospray ionisation mass spectrometer.

Monoclonal antibodies and their oxidised species are separated using YMC’s more hydrophobic BioPro HIC BF column, dedicated for low hydrophobicity proteins and oxidised mAb variants.

Four therapeutic mAbs of different species, isotypes, and pI together with their variants were analysed using pH and salt gradient mode using YMC’s non-porous BioPro IEX SF.

YMC-Triart Bio C4 is used in YMC capillary column format to analyse and identify intact commercial mAbs by micro LC–MS/MS at an elevated temperature of 75°C.

In this application note high-resolution SCX was coupled directly to an ultrasensitive online native MS using YMC’s BioPro IEX SF to analyse mAb charge variants.

Discover Coiled microextraction and SPME field collection and injection devices using portable GC–MS for separation, detection, and identification of explosive materials.

Vitamin C is essential for the repair of tissue and is thought to lower cancer risk. This application brief describes use of a Quasar biphenyl column in this analysis.

Plavix tablets contain the active ingredient clopidogrel hydrogen sulphate, readily analyzed by HPLC. Use of a Quasar C8 column in the analysis of Plavix is described.

Detect whisky and spirits adulteration. Field-portable GC–MS provides rapid determination of denatured alcohol when added to spirit drinks to raise alcohol content.

PharmaFluidics’ superior fabrication robustness of microfabricated LC columns results in sub-second retention time variation over three different μPAC™ capLC columns.

A CDS implemented appropriately and managed correctly can simplify system administration, ensure regulatory compliance and adherence to data integrity guidelines

Software solutions can guide all stages of pharmaceutical stress testing, from prediction of degradation chemistry to analytical development and, project management.

Synthetically derived food and beverage dye analysis to meet regulatory requirements aimed at reducing the risk of allergies, cancers, and attention deficits.

This application note demonstrates the ease of implementation and processing of a full method validation with a CDS using eWorkflow procedures based on ICH Guidelines.

First 220 nm laser in solid-state detectors overcomes DAD barriers for preparative chromatography with low limits of detection, no maintenance, affordability, and more.   

Analyzing crops for beneficial and harmful compounds is an indispensable tool for assessing the viability of various agricultural crops for consumption.

Teach a virtual chromatography lab with LC simulation software. See how one chemistry professor replaced hands-on labs with online experiential learning.

This white paper demonstrates the advantages of Fused-Core® technology in both comprehensive protein glycan characterization as well as a rapid and routine LC method for process control.

From siRNA to mRNA to DNA, separating full length product from impurities can be challenging. Taking into consideration the nature of the oligonucleotide you are interested in, you will be able to make informed choices about what approach to use to characterize or purify your molecule.