Application Notes: LC

This application note demonstrates the ease of implementation and processing of a full method validation with a CDS using eWorkflow procedures based on ICH Guidelines.

First 220 nm laser in solid-state detectors overcomes DAD barriers for preparative chromatography with low limits of detection, no maintenance, affordability, and more.   

Analyzing crops for beneficial and harmful compounds is an indispensable tool for assessing the viability of various agricultural crops for consumption.

Teach a virtual chromatography lab with LC simulation software. See how one chemistry professor replaced hands-on labs with online experiential learning.

This white paper demonstrates the advantages of Fused-Core® technology in both comprehensive protein glycan characterization as well as a rapid and routine LC method for process control.

From siRNA to mRNA to DNA, separating full length product from impurities can be challenging. Taking into consideration the nature of the oligonucleotide you are interested in, you will be able to make informed choices about what approach to use to characterize or purify your molecule.

Released & 2AB labelled N-glycans of similar structure, are shown to be well separated using HILIC methodology with the Avantor® ACE® Glycan column. 

In this publication, the concentrations of various amino acids in a culture fluid under different culture conditions were monitored using the Hitachi LA-8080 amino acid analyser.

To analyse fatty acids, they are derivatised to FAME’s for analysis by GC.  Here we separate 22 FAMEs with two CLA isomers using the Avantor® Hichrom HI-10 phase.

In this white paper we discuss the importance of correct mobile phase buffer selection and pH control in LC and LC–MS method development.

Our compilation of Avantor® ACE® applications for key analyte classes in cannabis products and potential contaminants provides technical support for this key expanding area.

One of the key parts of a robust HPLC method is one of the simplest HPLC vials. This guide breaks down this often difficult area and helps you find the compatible solutions for your instrument.

Here, we describe the utility of a new rugged HALO® SPP design for environmental PAH analysis using a highly selective phase offering excellent peak shape, resolution.

This application note demonstrates a high-resolution LC–UV method for the separation of 18 PAH compounds using the new HALO® PAH column with Fused-Core® technology.

This report looks at the determination of PAHs in food via LCMS analysis using QuEChERS extraction with isomeric separation in under 8 min using a new HALO® LC column.

This application demonstrates the performance improvement from the HALO® PAH column versus the leading competitor FPP column for the separation of PAH compounds.

This application demonstrates the performance of the new HALO® PAH SPP column in separating the 16 required PAH compounds in the EU 15+1 method in under 10 min.

This application note demonstrates the ability of the new HALO® PAH Fused-Core® HPLC column to perform an EPA 610+2 separation of 18 PAH compounds in 4.5 min.

Doxorubicin is a well-established chemotherapeutic agent frequently used in cancer treatment. Here we present the application of Electrical Asymmetrical Flow Field-Flow Fractionation (AF4) coupled with Multi Angle Light Scattering (MALS) to derive essential physico-chemical parameters, such as size distribution and surface charge, of a commercial liposomal Doxorubicin formulation.

Doxorubicin is a well-established chemotherapeutic agent frequently used in cancer treatment. Here we present the application of Electrical Asymmetrical Flow Field-Flow Fractionation (AF4) coupled with Multi Angle Light Scattering (MALS) to derive essential physico-chemical parameters, such as size distribution and surface charge, of a commercial liposomal Doxorubicin formulation.

• Automated HPLC modeling software can eliminate human error • Assessing the robustness of an analytical method • Getting a 360-degree view into the design space

Production of ethanol from biological sources produces side products, specifically propanol and methanol among other ketones, fatty acids, and esters.

Nine structurally-diverse, strongly basic drug compounds are resolved in less than 2 min using a high pH mobile phase and a fast acetonitrile gradient at 80 °C.