Application Notes: LC

This application note presents the characterization of ribosomal RNA using asymmetrical flow field-flow fractionation coupled with multi-angle light scattering.

This study describes the characterization of metal-containing nanoparticles in tattoo ink using AF4 coupled with multi-angle light scattering and ICP-MS.

This study describes the characterization of metal-containing nanoparticles in tattoo ink using AF4 coupled with multi-angle light scattering and ICP-MS.

Avoid precipitation of concentrated samples by splitting the pump channels: organic phase will pass through the autosampler; aqueous phase will join at the column head.

Avoid precipitation of concentrated samples by splitting the pump channels: organic phase will pass through the autosampler; aqueous phase will join at the column head.

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This application note shows how to achieve higher recoveries for commercially available MAbs using 15 % isopropanol as modifier in HIC mode.

μPAC™ technology has been used for analysing HCP impurities originating from recombinant expression systems used in the production of mAb based biotherapeutics.

The landscape for new biological drug development continues to evolve in complexity. With biosimilars entering the market and originator products coming off patent, the competitive race for market dominance is reliant on!

In this application we show how to achieve the best separation and sensitivity for insulin UHPLC-MS analysis by using formic acid as a weak ion pairing agent.

This application note shows how to achieve higher recoveries for commercially available MAbs using 15 % isopropanol as modifier in HIC mode.

The analysis of intact MAb and their fragments using the UHPLC compatible YMC-Pack Diol-200 column and specifically designed YMC-SEC MAB column is described.

The effective separation of intact antibodies from ADCs with their single DARs using hydrophilic interaction chromatography linked to UV/Vis is shown.

This application note demonstrates how to achieve robust chromatographic results for commercially available MAbs with higher sensitivity and sharper peaks at 80°C.

In addition to existing RP based methods, a method is presented for the determination of oligonucleotides using YMC’s strong, non-porous anion exchanger BioPro IEX QF.

This application note shows how to achieve robust chromatographic results for two commercially available MAbs using a modern temperature-stable widepore RP-phase.

The application shows that by implementing a metal-free column hardware any tailing caused by phosphate groups is avoided.

The method development for the simultaneous determination of highly hydrophobic Amyloid β peptides is described.

Describes a method for reproducible defined final volumes using several Vivaspin® centrifugal concentrators: Turbo 15, Turbo 4, and 500.

Outlines desalting or buffer exchange processes for Vivaspin concentrators to allow a significant reduction in salt concentration without diluting the protein sample.

Outlines a pre-treatment process for Vivaspin concentrators to improve recovery of very dilute protein samples in the nano- to microgram concentration range.

Outlines desalting or buffer exchange processes for Vivaspin concentrators to allow a significant reduction in salt concentration without diluting the protein sample.

Outlines a pre-treatment process for Vivaspin concentrators to improve recovery of very dilute protein samples in the nano- to microgram concentration range.

Describes a method for reproducible defined final volumes using several Vivaspin® centrifugal concentrators: Turbo 15, Turbo 4, and 500.

• Enhanced sensitivity and range are needed to obtain molecular weight and size. • New light scattering detector addresses flaws of traditional approaches.

This technical overview demonstrates automated method development of pharmaceutical samples using the Agilent 1290 Infinity II LC combined with ChromSwordAuto software.

This technical overview demonstrates automated method development of pharmaceutical samples using the Agilent 1290 Infinity II LC combined with ChromSwordAuto software.