Application Notes: LC

The analysis of intact MAb and their fragments using the UHPLC compatible YMC-Pack Diol-200 column and specifically designed YMC-SEC MAB column is described.

The effective separation of intact antibodies from ADCs with their single DARs using hydrophilic interaction chromatography linked to UV/Vis is shown.

This application note demonstrates how to achieve robust chromatographic results for commercially available MAbs with higher sensitivity and sharper peaks at 80°C.

In addition to existing RP based methods, a method is presented for the determination of oligonucleotides using YMC’s strong, non-porous anion exchanger BioPro IEX QF.

This application note shows how to achieve robust chromatographic results for two commercially available MAbs using a modern temperature-stable widepore RP-phase.

The application shows that by implementing a metal-free column hardware any tailing caused by phosphate groups is avoided.

The method development for the simultaneous determination of highly hydrophobic Amyloid β peptides is described.

Describes a method for reproducible defined final volumes using several Vivaspin® centrifugal concentrators: Turbo 15, Turbo 4, and 500.

Outlines desalting or buffer exchange processes for Vivaspin concentrators to allow a significant reduction in salt concentration without diluting the protein sample.

Outlines desalting or buffer exchange processes for Vivaspin concentrators to allow a significant reduction in salt concentration without diluting the protein sample.

Outlines a pre-treatment process for Vivaspin concentrators to improve recovery of very dilute protein samples in the nano- to microgram concentration range.

Describes a method for reproducible defined final volumes using several Vivaspin® centrifugal concentrators: Turbo 15, Turbo 4, and 500.

• Enhanced sensitivity and range are needed to obtain molecular weight and size. • New light scattering detector addresses flaws of traditional approaches.

This technical overview demonstrates automated method development of pharmaceutical samples using the Agilent 1290 Infinity II LC combined with ChromSwordAuto software.

This technical overview demonstrates automated method development of pharmaceutical samples using the Agilent 1290 Infinity II LC combined with ChromSwordAuto software.

ChromSword Auto® software communicates directly with the HPLC instrument to create a fully automated HPLC or UHPLC method development station

This Knowledge Note looks into the benefits of using sub 2µm silica particles, and helps to understand the relationship between smaller particle size and the effect on performance and column efficiency

In this white paper, learn how to accelerate HPLC/UHPLC method development and maximize selectivity with novel stationary phase chemistries

In this white paper, learn how to accelerate HPLC/UHPLC method development and maximize selectivity with novel stationary phase chemistries

In this white paper, we look at an intelligent solution for hydrophilic interaction liquid chromatography method development using complementary HILIC phases

See how the combination of ACE stationary phases with an offline version of Chromsword2 method development software can drastically aid method development

In this knowledge note we look at how the ACE method translation tool can be of use when translating methods from HPLC to UHPLC and vice versa

ChromSword Auto® software communicates directly with the HPLC instrument to create a fully automated HPLC or UHPLC method development station

This Knowledge Note looks into the benefits of using sub 2µm silica particles, and helps to understand the relationship between smaller particle size and the effect on performance and column efficiency

In this white paper, we look at an intelligent solution for hydrophilic interaction liquid chromatography method development using complementary HILIC phases

See how the combination of ACE stationary phases with an offline version of Chromsword2 method development software can drastically aid method development

In this knowledge note we look at how the ACE method translation tool can be of use when translating methods from HPLC to UHPLC and vice versa

• 4D LC offers significant benefits for biotherapeutic characterization. • Multidimensional LC can help find previously undetected impurities. • User-friendly software offers a solution to 4D-LC challenges.