Application Notes: Sample Prep
A Rapid, Simple, and Efficient Extraction Method for Additives in Plastics
January 10th 2019Plastics play a role in just about every market sector and research area including agriculture, food, packaging, pharmaceuticals, medical implants, and personal care products, making the potential leaching of additives like antioxidants or slip additives a major concern. In this article learn how the EDGE is able to extract additives from plastics in a fraction of the time of traditional techniques.
Estradiol Extraction From Serum Using SLE+
May 20th 2016This application note describes the use of ISOLUTE SLE+ for extraction of estrone (E1) and estradiol (E2) from human serum. Analysis is performed by an HPLC-MS/MS method that gives limits of detection of 0.001 ng/mL without derivatization or fluoride adduct stabilization. ISOLUTE SLE+ products provides high analyte recoveries, no emulsion formation and significantly reduced sample preparation time compared to traditional liquid-liquid extraction.
Determination of 11-nor-9-Carboxy-THC in Human Urine by QuEChERS and LC-MS/MS
April 25th 201611-nor-9-Carboxy-THC, also known as THCA or carboxy-THC, is the main secondary metabolite of THC (the active component of marijuana) formed in the human body [1]. THCA is excreted in urine in the form of glucuronide conjugates. THCA is not psychoactive but has a long half-life of up to several days or even weeks in very heavy users, thus determination of THCA in urine plays an important role in confirmation of marijuana consumption. The Substance Abuse and Mental Health Services Administration (SAMHSA) has set the THCA cutoff concentration of confirmatory testing at 15 ng/mL. Typical sample preparation methods for THCA in urine include liquid-liquid extraction (LLE) and solid phase extraction (SPE). This application utilizes a novel sample preparation technique, QuEChERS to effectively quantitate THCA levels in human urine.
Extraction Method for Pesticides in Poultry Fat
Using a targeted solid phase extraction (SPE) method and GC–ECD analysis, chlorinated pesticides are extracted from poultry fat resulting in a method that decreases the amount of labor and reagents when compared to the USDA-FSIS CHC2 method.
Extraction Method for Pesticides in Poultry Fat
Using a targeted solid phase extraction (SPE) method and GC–ECD analysis, chlorinated pesticides are extracted from poultry fat resulting in a method that decreases the amount of labor and reagents when compared to the USDA-FSIS CHC2 method.
Extraction of Cannabinoids from Brownies
April 7th 2016Cannabinoids are successfully extracted from a complex brownie matrix using the QuEChERS sample preparation technique, significantly reducing the presence of matrix interferences. The resulting clean samples are analyzed by GC–MS using a specialized deactivated GC column to provide heightened peaks for the cannabinoid compounds.
Improved Analysis of EPA Method 625
April 7th 2016EPA Method 625 is greatly simplified by using solid phase extraction (SPE) as an alternative to traditional liquid-liquid extraction (LLE). Using this SPE technique, paired with a sensitive GC–MS analysis, increases throughput and data quality, while decreasing manual labor and solvent usage.
Extraction of Cannabinoids from Brownies
April 7th 2016Cannabinoids are successfully extracted from a complex brownie matrix using the QuEChERS sample preparation technique, significantly reducing the presence of matrix interferences. The resulting clean samples are analyzed by GC–MS using a specialized deactivated GC column to provide heightened peaks for the cannabinoid compounds.
Improved Analysis of EPA Method 625
April 7th 2016EPA Method 625 is greatly simplified by using solid phase extraction (SPE) as an alternative to traditional liquid-liquid extraction (LLE). Using this SPE technique, paired with a sensitive GC–MS analysis, increases throughput and data quality, while decreasing manual labor and solvent usage.
Improvements in your LC–MS-MS Analysis
April 7th 2016Phospholipid removal proves to yield superior results as compared to a traditional protein precipitation step when analyzing a complex plasma matrix via LC–MS-MS. The results displayed a decrease in ion suppression, increased analyte sensitivity, and an improvement in column lifetime.
Improvements in your LC–MS-MS Analysis
April 7th 2016Phospholipid removal proves to yield superior results as compared to a traditional protein precipitation step when analyzing a complex plasma matrix via LC–MS-MS. The results displayed a decrease in ion suppression, increased analyte sensitivity, and an improvement in column lifetime.
Drug Analysis from Oral Fluids
April 7th 2016Using oral fluid as the matrix, a viable and simple solid phase extraction method for a wide range of drugs is developed. Several oral fluid collection devices were evaluated to determine the effectiveness of the cleanup procedure, ultimately confirming that the method is both robust and widely applicable.