Application Notes: Sample Prep
Characterization of Adeno-Associated Viral Vectors
Quality attributes of Adeno-Associated Virus (AAV) vectors are achieved with multi-angle light scattering coupled to separation methods and high-throughput dynamic light scattering.
Evaluation of Mixed-Mode Ion Exchange SPE for the Extraction of Opioids from Human Urine by UHPLC-MS/MS
This application notes introduces an SPE LC–MS/MS methodology using the Microlute™ CP SCX SPE 96-well microplate for the determination of 12 opioids from human urine.
Application Note: Hydrocarbon Fractionation Using Enviro-Clean Fusion®Ag+
This application note outlines the fractionation of hydrocarbons into aliphatic and aromatic fractions using UCT’s new silver-phase EPH fractionation cartridges.
Extractions of THC-metabolites from Urine
This application note demonstrates a sample preparation procedure for using the Empore C18 SPE cartridge for the extraction of the THC metabolite, THC-COOH, from urine samples.
Selection of UHPLC Columns for Nucleic Acid Oligomer Analysis
Synthesized 40-mer miRNA has been analyzed using both a conventional column and new types of UHPLC columns.
Scaling for Efficiency and Throughput: LC Method Optimization with Notes on Selectivity and Preparative LC
MHC-Associated Peptide Purification for Immunopeptidomics
Automated MHC-I is associated peptide enrichment using the Agilent AssayMAP Bravo platform with large-capacity Protein A cartridges for immunopeptidomics analysis.
Quantification and Characterization of Biotherapeutics Proteins
The AssayMAP Bravo was used to purify, digest, and clean up antibodies from 96 cell culture supernatants with high reproducibility and minimal labor prior to LC–MS.
Intact Antibody and Subunit Mass Determination
Intact and subunit antibody mass determination was streamlined using automated purification and enzymatic cleavage and deglycosylation of immobilized antibodies.
Assessing Multiple CQAs for Monoclonal Antibodies v2
Automated sample prep and LC–MS peptide mapping approach to characterize and quantify multiple monoclonal antibody (mAb) critical quality attributes (CQAs)