Application Notes: Sample Prep

This application note features a unique, simple, and robust solid-phase extraction method for cannabinoids in urine followed by analysis with LC–MS/MS.

This application note introduces a simple targeted extraction method for the analysis of nine nitazene compounds from urine and blood using the Clean Screen® DAU column.

Tecan’s proprietary NBE™ format enables digestion or incubation AND elution in a single SPE column. Read this app note to learn more and streamline your workflows!

The Clarity OTX 2 mg/well format is used for the isolation, characterization, and quantitation of current antisense oligonucleotide therapeutics from small biological fluids.

Advantages of using a supported liquid extraction (SLE) over liquid–liquid extraction (LLE) method for extracting a range of analytes from pig plasma.

Tecan’s proprietary NBE format SPE columns enable desalting in the column before peptide extraction. Read this app note to see how Tecan can accelerate your workflows!

Application note describing how thermal desorption–GC–MS is used to address multiple challenges in the detection of microplastics in different salt samples

Benzodiazepines are depressants and used to relieve anxiety, insomnia, and more. This app note discusses a urine extraction to confirm the presence of benzodiazepines.

A simple and robust method for the analysis of Opioids in biological matrices using UCT's Clean Screen® DAU SPE column and Selectra® DA UHPLC column.

This application notes introduces an SPE LC–MS/MS methodology using the Microlute™ CP SCX SPE 96-well microplate for the determination of 12 opioids from human urine.

This application note outlines the fractionation of hydrocarbons into aliphatic and aromatic fractions using UCT’s new silver-phase EPH fractionation cartridges.

This application note demonstrates a sample preparation procedure for using the Empore C18 SPE cartridge for the extraction of the THC metabolite, THC-COOH, from urine samples.

Synthesized 40-mer miRNA has been analyzed using both a conventional column and new types of UHPLC columns.

Automated MHC-I is associated peptide enrichment using the Agilent AssayMAP Bravo platform with large-capacity Protein A cartridges for immunopeptidomics analysis.

The AssayMAP Bravo was used to purify, digest, and clean up antibodies from 96 cell culture supernatants with high reproducibility and minimal labor prior to LC–MS.

Intact and subunit antibody mass determination was streamlined using automated purification and enzymatic cleavage and deglycosylation of immobilized antibodies.

Automated sample prep and LC–MS peptide mapping approach to characterize and quantify multiple monoclonal antibody (mAb) critical quality attributes (CQAs)

Automated phosphopeptide enrichment from cell lysates with Fe(III)-NTA enables the identification of thousands phosphopepides with high reproducibly and selectivity.

Automated phosphopeptide enrichment and ion mobility mass spectrometry (IMS-MS) enables detailed analysis of structural effects of site-specific phosphorylation.

Automated Antibody Drug Conjugate (ADC) purification and deglycosylation increases reproducibility and simplifies drug-to-antibody ratio (DAR) determination

Improved host cell protein analysis using highly reproducible and scalable automated sample digestion and fractionation ahead of LC–MS coupled with iterative MS–MS

The hydrolysis and SPE steps were combined for designer and pharmaceutical benzodiazepines from urine along with a fast quantitative LC–MS/MS method.

A low-level extraction of steroids using Novum PRO SLE is performed and compared with another SLE product for the baseline cleanliness.

This application note outlines the fractionation of hydrocarbons into aliphatic and aromatic fractions using UCT’s new silver-phase EPH fractionation cartridges.

Sampling and analysis of a challenging range of trace-level volatile & semi-volatile vapours of per- & polyfluorinated alkyl substances (PFAS) in air using TD–GC–MS

A simple and robust method for the detection of 16 cannabinoids in hemp and cannabis samples using a rugged isocratic HPLC method coupled with UV detection.

A solid-phase extraction method utilized to extract 19 synthetic cannabinoids produced recoveries in the range of 85–110% with corresponding RSD values of less than 5%.