John Chasse

An improved method for the extraction of proteins from human skin samples involved the acquisition of proteomic data through nano liquid chromatography tandem mass spectrometry (nano LC-MS/MS).

A recent study conducted by the University of Amsterdam (Amsterdam, Netherlands) and the University of Queensland (Queensland, Australia) developed a novel prioritization strategy that directly links fragmentation and chromatographic data to aquatic toxicity categories, bypassing the need for identification of individual compounds. LCGC International spoke to Viktoriia Turkina of the University of Amsterdam, lead author of the paper that resulted from this study, about their work.

A joint study between Selçuk University (Konya, Turkey) and Hitit University (Corum, Turkey) determined the natural occurrence and concentrations of the mycotoxins ochratoxin A (OTA) and deoxynivalenol (DON) in commercially available tortillas in Turkey. Contamination levels were quantified using validated analytical methods based on high-performance liquid chromatography (HPLC) coupled with fluorescence or ultraviolet detectors (HPLC-FLD or HPLC–UV).

Researchers from the University of Murcia (Murcia, Spain), the Carlos III Health Institute (Madrid, Spain), and the University of Siena (Siena, Italy) evaluated the concentration of polychlorinated biphenyls (PCBs) in chinstrap penguins and krill from Deception Island (South Shetland Islands, Antarctica) to provide additional data of the PCB presence in Antarctica. To this end, 34 samples of different tissues corresponding to four adult specimens and six chicks were analyzed with gas chromatography.

Researchers at the ICAR-Indian Agricultural Research Institute (New Delhi, India) investigated the endogenous levels of gibberellins (GAs) at six different developmental stages and four time points in four varieties of cauliflower using high-performance liquid chromatography (HPLC).

A recently published study set out to determine whether serum metabolic signatures inform on the relationship between healthy lifestyle and colon cancer risk. Serum samples were analyzed using liquid chromatography-mass spectrometry (LC-MS).

Can the peony, a plant having a long history of consumption and medicinal value in China, play a role in the treatment of type 2 diabetes mellitus? Researchers using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and high-performance liquid chromatography (HPLC) set out to find the answer.

A study conducted by researchers at the University of British Columbia (Vancouver, Canada) used gas chromatography coupled with flame ionization detection (GC-FID) to develop an automated sampling protocol applied to indoor rooms of a cannabis cultivation and a cannabis processing facility to investigate the temporal concentration profile and emissions of 22 terpenes.

In the final segment of our three-part interview with Jon Bardsley of Thermo Fisher, Jon discusses mitigation strategies and emerging tools to consider when testing for PFAS.

A study conducted by the College of Veterinary Medicine of Midwestern University (Glendale, Arizona), the University of California Davis School of Veterinary Medicine (Davis, California) and the College of Veterinary Medicine of the University of Tennessee (Knoxville, Tennessee) set out to describe the pharmacokinetics of oral gabapentin (GABA) in goats given as a single dose (SD) and multidose (MD) regimen, as well as to document any adverse effects after administration. Plasma samples were collected and analyzed via reversed-phase high performance liquid chromatography (RP-HPLC).

A joint study between the Nursing School of North Henan Medical University (Xinxaing, China) and The First Affiliated Hospital of Harbin Medical University (Harbin, China) used gas chromatography–mass spectrometry (GC-MS) for an observational study collecting and classifying surgical smoke generated during laparoscopic surgery to evaluate the carcinogenic risks to healthcare workers.

A recent review conducted by the Food Science Departments of Cairo University (Giza, Egypt), the University of Basrah (Basrah, Iraq) and Damascus University (Damascus, Syria) examined the wide array of bioactive compounds present in plant-based foods, with emphasis on their extraction, isolation, identification, characteristics, and emerging applications. These compounds were separated, purified, and analyzed from their complex plant-based matrices through a variety of chromatographic and spectrometric techniques.

Jon Bardsley of Thermo Fisher offers his perspective regarding the recent advances in PFAS detection in pharmaceutical manufacturing through the utilization of liquid chromatography-mass spectrometry (LC-MS), and factors that should be considered in achieving even greater advances.

Researchers at the University of Akron (Akron, Ohio) and Slippery Rock University (Slippery Rock, Pennsylvania) used gas chromatography-mass spectrometry (GC-MS) to analyze nicotine-free, cinnamon-flavored e-cigarette vapor to identify chemicals that might impact zebrafish cardiovascular development.

Researchers at the College of Life Science at Northwest Normal University (Lanzhou, China) used gas chromatography-ion mobility spectrometry (GC-IMS) to analyze the volatile organic compounds (VOCs) of olive oil extracted from eight maturity indices of Koroneiki olive fruits to determine the optimal harvest period.

Gas chromatography-mass spectrometry (GC-MS) was used in a joint study between the David Geffen School of Medicine at the University of California Los Angeles (Los Angeles, California) and the Department of Pediatrics at Boston Children's Hospital (Boston, Massachusetts) comparing parenteral nutrition–associated cholestasis, growth, and fatty acids in infants with gastrointestinal (GI) disorders who received either multioil intravenous lipid emulsion (MO ILE) or soybean oil lipid emulsion (SO ILE).

Jon Bardsley of Thermo Fisher offers his perspective regarding the advancement of PFAS detection in pharmaceutical manufacturing with liquid chromatography-mass spectrometry (LC-MS), and the influence of regulations in the pharmaceutical and biopharmaceutical industries.

Research conducted at Uskudar University (Istanbul, Turkey) explored the neuroprotective potential of ethanolic extracts of celery leaves, specifically in the treatment of Alzheimer’s disease. Liquid chromatography (LC) and mass spectrometry (MS)-based metabolomics analysis of the extract revealed the existence of a diverse array of secondary metabolites, including phenolic acids, hydroxycinnamic acid, flavonoids, flavonoid O-glycosides, flavonol, glycosides, and isoflavones.

A recent study aiming to investigate, for the first time, the biochemical and nutritional composition of the peels from five purple and two red potato cultivars and evaluate them as sources of healthy ingredients for the formulations of nutraceuticals quantified total phenolic content, carbohydrates, proteins, and lipids using UV–visible (UV-vis) spectrophotometry. The phytochemical composition was further characterized via high-performance liquid chromatography coupled with a diode-array detector (HPLC-DAD).

A joint study conducted by KU Leuven (Leuven, Belgium) and Wollo University (Dese, Ethiopia) aimed to develop and validate a liquid chromatography method with ultraviolet detection (LC-UV) for the identity screening and assay of 13 different injectable antibiotics in pharmaceutical formulations.

Researchers at the University of Otago (Dunedin, New Zealand) and the University of Auckland (Auckland, New Zealand) out to show that applying audible sound via a linear actuator, which impacted primarily on particle motion rather than the pressure component of audible sound, would significantly decrease beer fermentation time compared to control fermentations by keeping more viable yeast in suspension. Gas chromatography-mass spectrometry (GC-MS) was used to measure the abundance of volatile organic compounds (VOCs) in the beer samples.

Researchers at the University of Porto (Porto, Portugal) aiming to uncover novel therapeutic targets for bone loss of functionality and enhancing the understanding of its underlying bioenergetic mechanisms used liquid chromatography-mass spectrometry (LC-MS) to characterize proteomes.

Treated wastewater from three expedition North Atlantic Ocean cruise ships was collected and analyzed with a focus on pharmaceuticals, personal care products, and industrial chemicals. Samples were screened using data-dependent acquisition using liquid chromatography-high-resolution mass spectrometry (LC-HRMS).

A joint study conducted by the Henan University of Technology (Zhengzhou, China) and the Food Laboratory of Zhongyuan (Henan Province, China) developed and evaluated what the researchers deemed a novel zwitterionic stationary phase, Sil-DBO-PS, for hydrophilic interaction liquid chromatography (HILIC).LCGC International spoke to Ashraf Ali of the Henan University of Technology about this research and their findings.

Gas chromatography–mass spectrometry (GC–MS) was used to detect the chemical components of natural musk, harvested from forest musk deer at multiple stages of maturity. This research combines metabolomic profiling with biosynthetic pathway analysis to identify potential precursors and enzymes involved in musk biosynthesis.

Researchers at Baylor College of Medicine’s Children's Nutrition Research Center (Houston, Texas) developed and validated a high-throughput extraction and ultrahigh-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) method to separate and quantify 39 spinach flavonoid species in 11.5 min.

Cell membrane chromatography (CMC) technology was used by researchers in screening efficacy and antibacterial ability of articaine (AT) derivatives in the pre-emptive treatment of oral infections.

Two proof-of-concept hydrophilic interaction chromatography HILIC) methods were developed in a joint study conducted by the University of Geneva (Switzerland), Sanofi, and the Waters Corporation—one for disrupting LNPs and retaining the mRNA, and another for detecting only unencapsulated mRNA to assess encapsulation efficiency. LCGC International spoke to Jonathan Maurer, first author of the paper that resulted from this study, about the methods and the efforts that led up to their development.

Researchers at Andes Ag, Inc. (Alameda, California) established a high-throughput framework for the robust and precise quantification of soil inorganic carbon (SIC) from agricultural soils using gas chromatography (GC).

A joint study between the University of Tennessee (Knoxville, Tennessee) and the University of Pennsylvania School of Veterinary Medicine (Philadelphia, Pennsylvania) compared directly measured vitamin K1 (vitK1) concentrations in healthy dogs and dogs with chronic enteropathy (CE) using liquid chromatography tandem mass spectrometry (LC–MS/MS); they also investigated whether supplementation of vitK1 in dogs with CE would significantly increase vitK1 concentrations.