A sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method utilizing immunoaffinity enrichment to has been developed to quantify donanemab, an Alzheimer’s therapy, in human serum. The process involves immunoaffinity capture followed by tryptic digestion and targeted peptide quantification, and provides a robust, high-precision alternative to traditional immunoassays for monitoring therapeutic monoclonal antibodies in clinical settings.
Methods for Detecting and Characterizing PFAS in the Environment is scheduled to take place at Pittcon on Sunday, March 8, from 8:30 am -11:00 am in Room 303C of the Henry B. González Conference Center. Seven speakers from industry and academia will gather to explore and evaluate approaches for uncovering and distinguishing these chemicals to assess the potential human health and environmental risks they present.
The session Next-Generation Drug Development Enabled by Automation, Digitalization, and AI/ML is scheduled to take place at Pittcon 2026 in San Antonio, TX, on Monday, March 9, from 8:30 am–10:40 am in Room 304B of the Henry B. González Conference Center. A quartet of industry professionals will come together to share how automation and AI are revolutionizing modern drug development.
Researchers at the Catholic University of the Sacred Heart (Piacenza, Italy) used UHPLC-QTOF to profile phenolic compounds in forages and analyze metabolomics in milk used to produce Parmigiano-Reggiano cheese. IM-HRMS lipidomics revealed how different cow diets—such as grass-enriched versus alfalfa-based—modulate specific triacylglycerols, sphingomyelins, and plant-derived metabolites within the milk composition.
Researchers from the University of Michigan (Ann Arbor, Michigan) have introduced lack-of-fit (LOF), a peak-shape congruence measure that gauges the residual mismatch between candidate signals within a narrow retention window; pairs scoring below 20% are deemed degenerate and grouped together. LCGC International spoke to Caitlin Cain, lead author of the paper presenting this work, about her group’s research.
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was coupled with high-abundance protein depletion to identify potential biomarkers for rheumatoid arthritis (RA). Comparative proteomic profiling of RA and healthy sera revealed 154 differentially expressed proteins, and identified five potential RA-associated biomarkers, offering insights into RA pathogenesis and early diagnosis.
Using gas chromatography (GC) to analyze fatty acid (FA) profiles in milk from native goats, managed under grazing versus housed systems, the results indicated that grazing led to a more favorable FA profile, while housed goats produced higher milk yields.
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was employed to identify plasma biomarkers for global developmental delay (GDD) and intellectual disability (ID). High-resolution chromatography enabled the detection of key metabolites like 2-ketohexanoic acid and lipids such as diacylglycerol (16:0/16:0), which demonstrated exceptional diagnostic accuracy (AUC > 0.91), highlighting significant dysregulation in sphingolipid and amino acid metabolic pathways.
Research conducted at Iran’s Imam Khomeini Hospital utilized gas chromatography-mass spectrometry (GC-MS) to quantify serum opioid levels in 50 poisoned patients. Findings identified methadone as the primary causative agent (72%), with significant concentration variances observed across gender and residency. The analytical data from GC-MS proved critical for confirming clinical diagnoses and assessing poisoning severity.
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to quantify ondansetron concentrations in dogs following intravenous and subcutaneous administration. Analytical results revealed 84.6% bioavailability and peak plasma concentrations of 84.6 ng/mL. The high sensitivity of LC-MS/MS enabled precise non-compartmental modeling to establish therapeutic dosing intervals for clinical use.
To detect pesticide residue presence in grapes, researchers developed a high-throughput workflow utilizing low-pressure gas chromatography (GC) and ultra-high performance liquid chromatography (UHPLC) coupled with triple quadrupole mass spectrometry (QqQMS). This dual-platform approach enabled the detection of 236 pesticides, identifying 23 residues in grape samples and highlighting significant health risks from parathion-methyl through validated, high-precision quantification.
Korean researchers utilized liquid chromatography-tandem mass spectrometry (LC–MS/MS) to quantify serum metabolites in asthma patients. By leveraging the high-resolution separation of LC combined with the sensitive detection of MS/MS, researchers can identify specific metabolic signatures, providing critical insights into the biochemical pathways and potential biomarkers associated with asthma progression.
Researchers utilized liquid chromatography-tandem mass spectrometry (LC-MS/MS) and electronic tongue analysis to track protein degradation and taste compound evolution during roasting. The study identified six key differentiated metabolites (VIP > 1, P < 0.05), highlighting alanine and 5'-IMP as primary contributors to the significant umami and richness profiles developed during the final processing stages.
Researchers utilized headspace-solid phase microextraction coupled with gas chromatography-triple quadrupole mass spectrometry (HS-SPME-GC–MS/MS) to analyze 154 volatile organic compounds across 16 tomato varieties. By identifying 26 key aroma compounds through relative odor activity value (rOAV) analysis, researchers highlighted aldehydes as the primary VOC group. Furthermore, the integration of GC-MS/MS data with electronic nose technology and LightGBM machine learning demonstrates a sophisticated analytical framework for accurately discriminating tomato fruit color and flavor profiles.
Using ultrahigh-performance liquid chromatography high resolution tandem mass spectrometry (UHPLC–HRMS/MS), researchers at Wageningen Food Safety Research, part of Wageningen University & Research (The Netherlands) identified methyl 2-(sulfo-oxy)benzoate as a novel biomarker that differentiates pharmaceutical from plant-derived salicylic acid in eggs. LCGC International spoke to Serena Rizzo, corresponding author of the paper resulting from this work, about the group’s findings.
Research reveals critical challenges in transferring analytical high performance liquid chromatography (HPLC) methods to preparative fast protein liquid chromatography (FPLC) systems for therapeutic peptide purification. Scientists developed correction equations using impurity libraries to address parameter transferability issues, enabling more reliable scale-up from analytical testing to production-scale purification of peptide drugs like semaglutide and tirzepatide.
Using reverse-phase high-performance liquid chromatography (RP-HPLC), researchers separated tarantula venom into eleven fractions, revealing neuroactive compounds that mimic FDA-approved drugs. RP-HPLC-isolated fractions demonstrated donepezil-like, diazepam-like, and lidocaine-like neurobehavioral effects in zebrafish, highlighting spider venom's potential for neurological disorder therapeutics through chromatographic purification.
Gas chromatography-mass spectrometry (GC-MS) analysis of blubber and skin samples from bottlenose dolphins in the Saint Peter Saint Paul archipelago revealed diverse organohalogen compounds including polychlorinated biphenyls (PCBs), organochlorine pesticides (OCPs), brominated flame retardants (BFRs), and naturally produced methoxylated organobromines (MeO-BDEs), demonstrating long-range pollutant transport and establishing these marine mammals as effective sentinels for oceanic contamination monitoring.
Using reversed-phase and hydrophilic interaction liquid chromatography-mass spectrometry, researchers identified 256 metabolites in gingival crevicular fluid, revealing elevated purine degradation and ceramide metabolites alongside diminished oxy fatty acids in periodontitis patients, advancing precision diagnostics through untargeted metabolomic profiling of this chronic inflammatory disease.
Ultrahigh-performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-HRMS) was used in a research study to conduct comprehensive suspect screening across water, sediment, fish, wastewater, and sewage sludge samples from the Iberian Peninsula’s Tagus River basin, identifying 153 pharmaceutically active compounds, including cardiovascular, psychotropic, and pain management drugs between 2020 and 2022.
A study by Sungshin Women's University and Catholic University of Korea analyzed pre-prepared soup products using gas chromatography-mass spectrometry (GC-MS) to identify and quantify volatile compounds in commercial meat-based broths by comparing their chemical profiles, providing insights for developing authentic Korean-style home meal replacement products with optimized sensory characteristics.
A chromatography method developed to detect the antibiotic gentamicin in tiny ear fluid samples helps doctors precisely measure drug levels during inner ear treatments, ensuring patients receive safe, effective doses while fighting bacterial infections. This breakthrough is important because it allows precise measurement of life-saving antibiotics in microscopic biological samples, enabling safer treatments for serious ear infections.
To optimize sulforaphane (SFN) hydrolysis from broccoli seed hydrolysate (BSH), researchers employed liquid chromatography-high-resolution mass spectrometry (LC-HRMS) and high-performance liquid chromatography (HPLC). This chromatographic standardization enabled production of SFN-rich extracts for evaluating anti-obesity efficacy in high-fat diet-fed mice through multiple analytical techniques.
High-performance liquid chromatography (HPLC) was used by researchers to analyze carotenoid degradation during bread production, finding that xanthophyll esterification significantly improves stability. The study examined wheat genotypes and processing conditions, identifying baking as the critical degradation step and recommending technological innovations combined with genetic improvements to enhance carotenoid retention in wheat-based products.
To enable robust investigation of microbiota–host metabolic interactions, a targeted gas chromatography–tandem mass spectrometry (GC–MS/MS) method was developed and validated by a research group at the European Molecular Biology Laboratory (Heidelberg, Germany) for the simultaneous quantification of 120 chemically diverse gut microbiota–derived metabolites across multiple biological matrices. LCGC International recently spoke to Nikita Denisov and Michael Zimmermann, two of the authors of a paper resulting from this work.
Liquid chromatography-mass spectrometry (LC–MS) quantified dexamethasone release from coated cochlear implant carriers, showing sustained intracochlear delivery, basal–apical distribution, and improved pharmacokinetics versus fully loaded silicone rods after implantation.
A recent review article evaluates chromatographic and complementary analytical strategies for quantifying cardiovascular micro- and nanoplastics in complex human matrices. The authors contrasts particle-resolved vibrational methods with mass-based chromatographic approaches, highlighting strengths, limitations, and clinical relevance for deriving reliable polymer-specific exposure metrics linked to cardiovascular pathology and outcomes in translational cardiology research settings.
Researchers compared oral microbiota, salivary cytokines, and short-chain fatty acids in persistent Covid disease using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Age- and sex-matched analyses showed no significant SCFA differences after correction, though valeric acid trended higher. Cognitive impairment and fatigue were greater in patients, highlighting chromatography’s role in probing subtle metabolic signals variations overall.
Total knee arthroplasty alters endogenous pain-regulating pathways, including the endocannabinoid system. Using validated liquid chromatography–mass spectrometry (LC-MS), perioperative cerebrospinal fluid and plasma endocannabinoids were quantified in patients with knee osteoarthritis. Distinct endocannabinoid profiles were associated with acetaminophen exposure, surgery, and postoperative pain outcomes.
Using untargeted liquid chromatography-mass spectrometry (LC–MS) metabolomics, researchers compared black and white mantle regions of the Pacific oyster, identifying 527 differential metabolites.
