John W. Dolan

Lloyd Snyder was one of perhaps ten “founding fathers” of high performance liquid chromatography (HPLC), with seminal publications in most areas, including adsorption (normal phase), reversed phase, isocratic, gradient, and preparative chromatography; plus solvent, temperature, and column selectivity. With nine books, several hundred publications, and an h-index of 83, he was one of the most widely cited chromatographers and received many of the most prestigious awards in separation science.

Lloyd Snyder was one the “founding fathers” of high performance liquid chromatography (HPLC). He was one of the most widely cited chromatographers with many important contributions to the field of separation science.

In advance of PittconIn advance of Pittcon 2018, leading scientists-Ronald Majors, Richard Henry, John W. Dolan, Zachary S. Breitbach, and Daniel W. Armstrong-who will be speaking at the LCGC awards symposium give us a preview of their talks.

Here are five ideas to help avoid common problems.

John shares lessons learned from his more than three decades of teaching liquid chromatography practitioners and helping them solve their problems.

Want to improve column life? Here are some ideas.

Simple ways to estimate chromatographic changes.

Have a problem with your LC system? Maybe applying a little “DDT (Don’t Do That)” may help.

A full measure of tips for reliable gradient methods.

Preventive maintenance compressed.

In his final column before retirement, John Dolan reflects on the changes he has seen during his 34 years as the author of “LC Troubleshooting,” and shares his short list of best practices that still hold true today.

In his final column before retirement, John Dolan reminisces about his years as the author of “LC Troubleshooting”, and some of the changes that have taken place in liquid chromatography (LC) technology during that time.

How do non-alkylsilica columns provide us an additional dimension of column selectivity?

How do non-alkylsilica columns provide us an additional dimension of column selectivity?

When developing or modifying an LC separation, a common strategy is to change selectivity by choosing a new column. Here’s guidance for making that selection.

You have to be careful to avoid unwanted results when adjusting gradient conditions.

You have to be careful when adjusting gradient conditions.

When you want to adjust a United States Pharmacopeia (USP) method for a different size column or to meet system suitability criteria that fail, how much of a change can you make without revalidating the method?

When you want to adjust a United States Pharmacopeia (USP) method for a different size column or to meet system suitability criteria that fail, how much of a change can you make without revalidating the method?

How much of a change can you make to a USP method without revalidating the method?

Several variables can be used to change selectivity in a liquid chromatographic (LC) separation. Here we compare the variables in an effort to prioritize which experiments will be most effective.

Several variables can be used to change selectivity in a liquid chromatography (LC) separation. Here we compare the variables in an effort to prioritize which experiments will be most effective.

We’ll see how to find the “sweet spot” in terms of retention for a liquid chromatographic separation as well as how much retention change is to be expected for a selected change in mobile-phase percent organic or column temperature.