Phenomenex, Inc.

In this application note, we profile capsid protein peptides from AAV5 and AAV9, digested with trypsin using a Biozen™ Peptide XB-C18 reversed phase column.

We present an LC-HRMS approach to distinguishing a pool of multiple caps and their relative integrity of digested mRNA oligonucleotide strands of 1250 nucleotides.

SEC is highlighted as a tool for the separation and detection of higher-order structure of single stranded DNA oligonucleotides.

Review profiling of denatured and dissociated capsid proteins from AAV5 and AAV9, via analysis with LC-MS using a Biozen™ Intact XB-C8 reversed phase column.

Webinar Date/Time: Wed, Sep 27, 2023 11:00 AM EDT

Webinar Date/Time: Thursday, September 21, 2023 at 11am PST | 2pm EST | 6pm GMT| 8pm CEST

A breakthrough for adsorbable and extractable organic fluorine (AOF and EOF) and how different SPE phases are used for accurate determination of PFAS.

Webinar Date/Time: Tuesday, July 25th, 2023 at 11am EDT | 8am PDT | 4pm BST | 5pm CEST

Webinar Date/Time: Wed, May 31, 2023 11:00 AM EDT

Webinar Date/Time: Wed, Mar 15, 2023 11:00 AM EDT

Webinar Date/Time: Friday, December 9th, 2022 at 11am EST|8am PST|4pm GMT|5pm CET

How to speed up sample prep time and equivalent acccuracy and precision.

Monday, October 24, 2022 Session One : 9:30am EDT | 6:30am PDT | 2:30pm BST | 3:30pm CEST Session Two : 12:30pm EDT | 9:30am PDT | 5:30pm BST | 6:30pm CEST Are your analytical methods keeping up with the pace of new biotherapeutic drug modalities? Attend this symposium to ensure success!

Wednesday, October 19th 2022, 11am EDT | 8am PDT | 4pm BST | 5pm CEST In this webinar, we will give an overview of sample preparation and LC-MS strategies for synthetic oligonucleotides, including how to adjust experimental design depending on the oligo and sample matrix. Additionally, novel small volume clean-up solutions for oligonucleotides will be explored to further optimize challenging methods.

We demonstrate the utility of size-exclusion chromatography (SEC) for the detection of high-order structure of single-stranded DNA oligonucleotides.

The use of resistor tubing and 200 mM ammonium acetate in the mobile phase was implemented to measure loading capacity of a Biozen 1.8 μm dSEC-2 column.

Protein standards were explored with known hydrodynamic radii, showing how this can be used to determine ideal size exclusion conditions for optimal method performance.

Using standard phosphate containing buffers with different percentages of isopropanol for aggregate analysis of an antibody-drug conjugate (ADC), Kadcyla

Temperature, gradient program, and flow rates are explored and optimized for intact mass applications for mAbs when using Biozen WidePore C4 column with bioTi hardware.

The hydrophilic nature of the Biozen dSEC-2 stationary phase shows favorable aggregate profiles for forced degradation work under high salt conditions.

The Clarity OTX 2 mg/well format is used for the isolation, characterization, and quantitation of current antisense oligonucleotide therapeutics from small biological fluids.

Application guide to advanced sample preparation for chromatography analysis and mass spectrometry techniques for PFAS's more difficult matrices.

Laboratory productivity increase is demonstrated using an SDVB polymer SPE and C18 column selectivity.

Multiple laboratories applied method EPA 537.1 and 533 for the extraction of PFAS in pristine non-potable water and source water assessment.

PFAS experts provide insight on workflow considerations on how to manage and prevent cross-contamination to ensure quality analysis.