Special Issues
Column packing materials continue to evolve as user needs for highthroughput, high-resolution and high-sensitivity HPLC analyses drive further developments. In this introductory article to this supplement, Ron Majors covers basic column packing morphology and particle design and compares and contrasts modern HPLC columns. Some applications of modern HPLC columns are provided. Future directions in packing developments are predicted.
Special Issues
Monoliths are separation media in the format that can be compared to a single large "particle" that does not contain interparticular voids. As a result, all the mobile phase must flow through the stationary phase. This convective flow greatly accelerates the rate of mass transfer. In contrast to diffusion, which is the typical driving force for mass transfer within the pores of particulate stationary phases during chromatographic processes, convective flow through the pores enables a substantial increase in the speed of the separation of large molecules such as proteins. A thorough theoretical treatment of the mass transfer within monolithic materials has been developed by Liapis (1) and Tallarek (2).
Special Issues
Ion exchange is one of the older of the chromatographic techniques yet each year new products continue to hit the market. In this paper, Chris Pohl of Dionex will summarize some of the stationary phases that have been developed for modern ion-exchange and ion chromatography. He will focus on phase design and then turn his attention to new anion and cation columns introduced in the last couple of years.
The Column
Biologists entering liquid chromatography-mass spectrometry (LC-MS) from a background of general LC rarely use their mass spectrometer to its full potential. Isotope peaks offer huge possibilities both in semi-quantitative interpretation of structure and in quantitative labelling studies. This article examines charge state, "easy" isotopes, such as chlorine, the slightly harder problem of sulphur compounds, and finally looks at a method for improved measurement of heavy labels in a metabolic study.
The Column
A new method for the on-line solid-phase extraction of polycyclic aromatic hydrocarbons (PAH) from water samples followed by high performance liquid chromatography with fluorescence and UV detection was developed. The solid-phase extraction was optimized on a polymer-based sorbent that features a unique polar-enhanced technology.
The Column
News All the news from April 2006 Opinion Zosimus describes chromatography as a "thinking" analytical science and tells us to put more thought into it. Market Trends & Analysis Glenn Cudiamat provides a report on pharmaceutical market demand for life science instrumentation. Highly selective detection and identification of nitrofurans metabolites in honey using LC-MS-MS. Eduardo Matus, Jean-Jacques Dunyach and Alejandro Albornoz Radiochromatography and its applications Duncan Carmichael Q&A Don Kuehl of Cerno Bioscience talks to The Column about a new software package for mass spectrometers.
LCGC Europe
Is it possible to combine a serious scientific meeting together with social events and fun? Well if it is possible then the ExTech and HTC-9 meeting held in York, UK between 6?10 February came very close.
LCGC Europe
Sony has released details of its new e-book reader using E-Ink technology. This could transform the electronic reading experience. The lightweight reader could bring a whole new meaning to "book smart".
LCGC Europe
The department of Chemistry and Chemical Biology at Harvard University has purchased a site licence of the ACD/2D NMR processor. The Advanced Chemistry Development (ACD/Labs) processor will be deployed to all faculty staff and students for use on department-owned or leased computers as well as personal PCs.
LCGC North America
LCGC North America
LCGC North America
LCGC North America
LCGC North America
LCGC North America
LCGC North America
The Column
Proteomics research is an active and dynamic field, but as many of the abundant protein classes have been investigated thoroughly, the move to low-abundance targets has posed significant difficulties. The problem of purifying proteins expressed in cellular organelles is of particular interest. However, extensive separation, fractionation, and enrichment are required to obtain those low-abundance proteins that drive life processes in these subcellular entities. Here, we review the use of continuous-flow ultracentrifugation for the purpose of organelle separation.
The Column
In this article, the authors detail a liquid chromatographic instrument system that successfully masters the many challenges of sub-2-mm liquid chromatography.
