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The authors discuss zero-, first- and second-order data and describe the advantages of using second-order chromatographic data compared with the more traditional univariate or multivariate calibration. They demonstrate how its use can uncover much more information from chromatograms and spectra.

Isotopic analysis of archaeological materials provides information on age and source, but analysis of single compounds isolated from artefacts can provide more accurate measurements and additional details. In this article, the authors provide a review of chromatographic separations of archaeological biomolecules for their analysis using accelerator mass spectrometry or isotope ratio mass spectrometry. The article concentrates primarily on studies involving carbon and nitrogen.