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LCGC June 2006 BPA

Tosoh Corporation (Montgomeryville, Pennsylvania) announced that as part of strategic initiatives to take advantage of high-growth bioscience-related markets, ?Scientific Instruments Division? will be renamed ?Tosoh Bioscience Division.?

News from the chromatography industry.

Upcoming educational events in the chromatography industry.

Upcoming conferences and events in the chromatography industry.

In this month's issue features two new articles entitled "Accurate Mass Compound Identification with Single-Quadrupole Gas Chromatography-Mass Spectrometry" and "A Chromatographic Screening Method to Identify Agents that Reverse Intrinsic Drug Resistance in Colon Cancer Cells."

Conjugation catalysed by the UDP-glucuronosyltransferase (UGT) superfamily of drug-metabolizing enzymes is an important mechanism of anticancer drug resistance in colon cancer cells. The mechanism manifests itself by a reduction in the intracellular concentrations of the parent drug through increased export of the glucuronide metabolites to the extracellular compartment.

Thermo Electron (Waltham, Massachusetts) announced the acquisition of Fisher Scientific International Inc. (Pittsburgh, Pennsylvania).

PerkinElmer, Inc. (Shelton, Connecticut) announced the acquisition of Spectral Genomics, Inc. (Houston, Texas), a leading company in molecular karyotyping technology used to evaluate chromosomal abnormalities.

News All the news from June 2006 Opinion Zosimus looks at the image chromatography has amongst the next generation of separation scientists and tells us why we should forget the past and look to the future. Market Trends & Analysis Glenn Cudiamat updates us on the growing importance of analytical instrumentation to homeland security. Country focus This month we look at some of the latest innovations from Scandinavia. Reversed-phase column selectivity Uwe D. Neue, Bonnie A. Alden, Susan Corman and Joe C. Arsenault. USP analysis of tetracylines using reversed-phase polymeric HPLC columns Linda L. Lloyd and Keeley J. Mapp Supplies & Services Monolith columns for fast, high-resolution HPLC Srinivasa Rao, Jim Thayer and Ilze Birznieks Q&A John Christensen of ESA Biosciences speaks to The Column about its brand new breed of universal HPLC detector based on aerosol discharging.

Thermo, Chata, and Cerno take top awards - Bruker Biosciences to acquire Bruker Optics - Chinese pharmaceutical company selects Thermo - DataApex and Knauer annouce cooperation.

Heidi Grecsek, PerkinElmer Life and Analytical Sciences

Higher productivity and faster analyses are two of the driving forces for continued improvement in high performance liquid chromatography (HPLC) column technology. Reduction in the average particle size of HPLC porous column packings below 2 ?m has resulted in sub-1.0 min separations in the gradient and isocratic modes. In this instalment of "Column Watch", Ron Majors traces the development of particle technology from the beginning of HPLC to the present, discusses why small particles are desirable, and probes some of the difficulties to be encountered, including extracolumn band broadening, pressure restrictions, and instrumental considerations. Finally, he shows a wide variety of fast- and ultrafast applications examples from commercial products in the sub-2 ?m range. Speculation on future directions in HPLC in particle technology concludes the column.

Conjugation catalysed by the UDP-glucuronosyltransferase (UGT) superfamily of drug-metabolizing enzymes is an important mechanism of anticancer drug resistance in colon cancer cells. The mechanism manifests itself by a reduction in the intracellular concentrations of the parent drug through increased export of the glucuronide metabolites to the extracellular compartment. Modulation by an inhibitor of UGT inhibits the formation of metabolites and restores intracellular concentrations of the drug. This article describes a screening method using HT29 human colon cancer cells and based on HPLC methodology that allows the identification of effective modulators of the glucuronidation mechanism of drug resistance. A rapid solid-phase sample preparation technique using C2-bonded 40 ?m silica particles was developed for the extraction of cell lysates and culture media without degradation of unstable parent compounds and their glucuronides or artefactual in situ formation of metabolites.

New ways of envisioning scientific data are constantly being developed in data rich research environments. Researchers at the University of California, Irvine's Center of GRAVITY (Graphics, Visualization and Imaging Technology), USA have developed a new display for this data rich market. They have developed the Highly Interactive Parallelized Display Wall or HIPerWall. It is a massively tiled, grid-based display built using fifty 30-inch Apple cinema displays and twenty-five Power Mac G5 computers. This powerful display system allows researchers to view and manipulate their data sets at extremely high resolutions and collaborate with other scientists in new and exciting ways.

Thermal conductivity detectors have been in use since before the beginning of gas chromatography. Essential for fixed-gas detection - no substitute has the same ease of use and stability - thermal conductivity detectors are also employed when the auxiliary or combustion gases required by flame ionization or other detectors are unsafe or impractical. Although they cannot match the sensitivity of ionization detectors, thermal conductivity detectors are the third most used detector, surpassed only by flame ionization and bench-top mass-spectrometry detectors. This month's instalment of "GC Connections" takes a look at the operating principles and inner workings of the thermal conductivity detectors.

Infotrieve has announced that the University of Pittsburgh School of Medicine and Acacia Research's CombiMatrix Molecular Diagnostics (CMD) group have each adopted the company's electronic laboratory notebook (ELN). This software solution is said to be the first enterprise-wide ELN to receive the full endorsement of the collaborative electronic notebook systems association (CENSA).

In terms of practical use of the results, resolution is generally more critical than retention.

In this article, the selectivity changes that can be obtained with alkyl chain, polar endcapped, cyano, and perfluorinated chemistries when using generic mobile phases are illustrated for basic, acidic and neutral analytes.

Upcoming educational events from the chromatography industry.