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Guest columnist Matt Przybyciel reviews the structure, behaviour and applications of both alkyl- and phenyl-fluorinated phases in HPLC. The author presents some guidelines on potential areas of application for alkyl and phenyl-fluorinated phases, in which conventional C8, C18 and phenyl phases might have insufficient selectivity or too strong retention.

Improvement of detection and quantification limits for LC methods is a result of taking advantage of all the tools you have at your disposal.

Thermo Electron Corporation (Waltham, Massachusetts) has recently announced the opening of a customer demonstration laboratory in Mumbai, India. The center will serve local pharmaceutical, petroleum/petrochemical, automobile, environmental, and agricultural market sectors and will be equipped with demonstration and training facilities.

Thermal conductivity detectors have been in use since before the beginning of gas chromatography. Essential for fixed-gas detection - no substitute has the same ease of use and stability - thermal conductivity detectors also are employed when the auxiliary or combustion gases required by flame ionization or other detectors are unsafe or impractical. Although they cannot match the sensitivity of ionization detectors, thermal conductivity detectors are the third most used detector, surpassed only by flame ionization and bench-top mass-spectrometry detectors. This month's installment of "GC Connections" takes a look at the operating principles and inner workings of the thermal conductivity detectors.

In this month's "Column Watch," Ron Majors answers HPLC column questions posed by readers and others.

An important prerequisite of a good sampling procedure for skin sebum is its reproducibility.

This article presents an overview of the work performed to define generic separation strategies and methods in chiral method development using capillary electrochromatography as a separation technique. Polysaccharide chiral stationary phases were found suitable for this purpose. Two separate strategies were defined, one for acidic and one for non-acidic substances. These strategies were evaluated and found applicable on structurally diverse molecules, showing their generic character.

Polystyrene-divinylbenzene (PS-DVB) trap columns have been evaluated for microcolumn switching applications. In contrast to traditional stationary phases, which consist of packed particles, the monolithic separation medium is made of a continuous, rigid polymeric rod with a porous structure. The absence of intraparticular void volume increases separation efficiency, allowing for faster separations. Column lifetime is higher compared to packed columns.

Dionex has developed an autosampler with very precise sample preconcentration capabilities. When analyte detection is no longer possible even with a large loop injection, sample preconcentration (loading a sample through a concentrator column and eluting the concentrate) is used.

News All the news from December 2005 Opinion This month Zosimus calls for a standard interface across all analytical instrumentation and presents his arguments for such a change. Market Trends & Analysis Glenn Cudiamat provides an insight into the process chromatography market with a particular look at the growing biotechnology sector in Japan. Country focus In this issue, we end the year with a focus on Japan and review some of the projects in place. Q&A Tuulia Hyotylainen from the University of Helsinki speaks to The Column about her research in multidimensional chromatography. Supplies & services

Contents:- Recent Developments in Columns for UPLC- Transfer of the USP Human Insulin Related Compounds HPLC Method to the ACQUITY UPLC System- Validation of a UPLC Method for a Benzocaine, Butamben, and Tetracaine Hydrochloride Topical Solution- Meeting Analytical Challenges with UPLC-MS- Enabling Significant Improvements for Peptide Mapping with UPLC- Analysis of Prostaglandins with the Quattro Premier XE

This column deals with a few chemists active during the early growth of the petroleum industry who carried out chromatography-like investigations even before Tswett...

Varian acquires Polymer Labs, Thermo contributes to Hurricane Katrina Disaster Relief Fund, and French Police select Whatman for DNA collection.

By taking advantage of the benefits provided by normal-phase mode, highly productive and cost-effective strategies for high-throughput purification of drug discovery products have been developed in the analytical laboratories at Lilly-Spain. The straightforward scaling-up of generic protocols from an analytical to a preparative scale has yielded successful results not only when working in HPLC but also when transferring conditions to other standard low and medium pressure chromatographic systems that are routinely used by synthetic chemists.

The micropipette tip containing solid phases is a relatively new sample preparation format that permits the handling of microlitre and submicrolitre amounts of liquid samples using the techniques of solid-phase extraction, dialysis and enzyme digestion. Phases are packed, embedded or coated on the walls of the pipette, permitting liquid samples to be moved and transferred without undue pressure drop or plugging. This column reviews the latest technologies in micropipette tip sample preparation used in the study of genomics, proteomics and metabolomics.