Mass Spectrometry

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Silvia Radenkovic is a laboratory clinical biochemical genetics fellow at UMC Utrecht, the Netherlands and a member of Females in Mass Spectrometry (FeMS). She is currently doing a clinical fellowship where she is involved in diagnosing and helping treat patients with inborn metabolic diseases (IMD). Her research focuses on IMD such as congenital disorders of glycosylation (CDG), omics techniques such as tracer metabolomics, and different disease models.

This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.