Heat and High pH Forced Degradation of Antibodies on a Biozen dSEC-2 Column
January 22nd 2024This application note demonstrates the applicability of the Biozen dSEC-2 column in understanding the biophysical integrity monoclonal antibodies and related proteins. The hydrophilic nature of the Biozen dSEC-2 stationary phase shows favorable aggregate profiles for forced degradation work under high salt conditions which is a known challenge when working with more hydrophobic hybrid particles for these studies.
Size Exclusion Chromatography for the Determination of Protein
January 22nd 2024An understanding of analyte hydrodynamic radius (Rh), as well as size exclusion column pore size and distribution, are all critical in the understanding of how an analyte will behave by SEC. In this white paper, we will explore several protein standards with known hydrodynamic radii, and how this can be used to determine ideal size exclusion conditions for optimal method performance.
Novel Oligonucleotide Sample Clean-up and Method Optimizations
October 19th 2022Wednesday, October 19th 2022, 11am EDT | 8am PDT | 4pm BST | 5pm CEST In this webinar, we will give an overview of sample preparation and LC-MS strategies for synthetic oligonucleotides, including how to adjust experimental design depending on the oligo and sample matrix. Additionally, novel small volume clean-up solutions for oligonucleotides will be explored to further optimize challenging methods.