LCGC Europe-06-01-2019

This is the fifth in a series of articles exploring current topics in separation science that will be addressed at the HPLC 2019 conference in Milan, Italy, from 16–20 June.

The effect of dwell volume on chromatographic selectivity can be successfully modelled using retention prediction software. Hence, the robustness of reversed-phase LC gradient methodologies, with respect to dwell volume, can be conveniently assessed.

Adding an inline mixer between the sample injector and column in a liquid chromatography (LC) system can be an effective way to resolve problems with peak shape caused by the sample diluent.

Monoclonal antibodies (mAbs) are being developed at an explosive rate and have attracted great interest from both smaller biotech firms and big pharmaceutical companies. Developing mAbs and next-generation antibody–drug conjugates (ADCs) is highly demanding in many ways. From an analytical perspective, handling mAbs and ADCs presents many new challenges. This article describes how size-exclusion chromatography (SEC) combined with high-resolution mass spectrometry (HRMS) can be applied to the detailed characterization of mAbs and ADCs.

Tomas Cajka previews his keynote lecture at HPLC 2019, where he will introduce an LC−MS workflow (LIpids, Metabolites and eXposome compounds [LIMeX]) for simultaneous extraction of complex lipids, polar metabolites, and exposome compounds that combines LC–MS targeted and untargeted analysis.

Davy Guillarme previews his presentation at HPLC 2019 highlighting the new trends in LC×LC applied for biopharmaceutical characterization, including the hyphenation with high‑resolution mass spectrometry (HRMS) and ion mobility spectrometry (IMS).

Rawi Ramautar discusses capillary electrophoresis–mass spectrometry for metabolomics and previews the topic for HPLC 2019.

Anneli Kruve previews the topic of nontargeted liquid chromatography–high-resolution Mass spectrometry for HPLC 2019.

Barbara Bojko previews the topic of solid-phase micoextraction for clinical and pharmaceutical research.

Achille Cappiello previews the topic and the benefits of liquid-electron ionization liquid chromatography–mass spectrometry interfaces for HPLC 2019.

The 11th Encontro Nacional de Cromatografia (11ENC) will be held 9–11 December 2019 at Hotel Aldeia dos Capuchos, Congress Centre, in Caparica, Portugal.

A guide to solving recovery problems in solid-phase extraction (SPE)

Click the title above to open the LCGC Europe June 2019 regular issue, Vol 32, No 06, in an interactive PDF format.

Wouldn’t it be nice if a single generic high performance liquid chromatography (HPLC) method could be used for all small molecule drugs - not only for potency assays but also for ICH-compliant stability-indicating assays?