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© 2020 MJH Life Sciences™ and Chromatography Online. All rights reserved.
September 01, 2014
Supercritical (subcritical) fluid chromatography (SFC) was evaluated as an alternative to HPLC for the enantiomeric separation of primary amines on a cyclofructan-based chiral stationary phase.
A discussion of the role of the gradient in separation by reversed-phase and ion-exchange HPLC, with data from two products to illustrate the key points
Understanding the chemistry of embedded polar group and perfluorophenyl phases leads to better decisions during method developement
A review of the essential elements of MS approaches to protein quantification and a critical comparison of the available options
An investigation into some "typical" HPLC method specifications to highlight ways of critically evaluating the major and minor parameters of a method
How can resolution be determined when peak width cannot be measured?
Click the title above to open the LCGC North America September 2014 regular issue, Vol 32 No 9, in an interactive PDF format.