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Click the title above to and turn to page 67 to open the LCGC North America February 2020 Application Notebook, Volume 38, Number s2, in an interactive PDF format.

Click the title above to open the LCGC North America February 2020 issue, Volume 38, Number 2, in an interactive PDF format.

In situ measurements of the mobile-phase pH before and after the column help to rationalize the effects of mismatch in pH and concentration between the mobile phase and sample buffer mismatch in reversed-phase LC separations.

At the 43rd International Symposium on Capillary Chromatography (ISCC 2019) in Fort Worth, Texas, a panel addressed the current challenges and potential future directions in capillary liquid chromatography. How can their vision be achieved?

Hybrid chromatography data systems are the worst possible solution for any laboratory, whether regulated or unregulated, and yet they are pervasive. Here’s why.

Daniel W. Armstrong and Szabolcs Fekete are the winners of the 13th annual LCGC Lifetime Achievement and Emerging Leader in Chromatography Awards, respectively. Here, we review their achievements.

If I inject a sample buffered at a pH different from that of the mobile phase, how quickly is the sample buffer neutralized inside the column?

The “State-of-the-Art in Capillary Liquid Chromatography” panel discussion at the 43rd International Symposium on Capillary Chromatography (ISCC 2019) in Fort Worth, Texas, USA, was a thoughtful dialogue on current challenges and potential future directions in the field. The session included a general overview of the current state of the field, key drawbacks preventing widespread use of capillary liquid chromatography (LC) columns, and how these challenges might be overcome. In this article, we highlight some of the common themes that were discussed as part of this panel.

The International Trade Fair for Laboratory Technology, Analysis, Biotechnology and Analytica Conference 2020 will take place in the International Congress Center (ICM), Munich, Germany, from 31 March to 3 April 2020.

SepSolve Analytical Ltd has successfully relocated its headquarters to a new and larger premise in Peterborough, UK.

Researchers have developed a headspace gas chromatography-ion mobility spectrometry (HSGC–IMS) method to detect volatile organic compounds (VOCs) linked to Alzheimer’s disease in faeces and urine.

Food safety scientists have validated a new method to detect neurotoxins in seafood products using UHPLC–HILIC–MS/MS.

Click the title above to open the LCGC Europe January 2020 regular issue, Volume 33, Number 1, in an interactive PDF format.

Click the title above to open The Column January 2020 Europe & Asia issue, Volume 16, Number 1, in an interactive PDF format.

Click the title above to open The Column January 2020 North American issue, Volume 16, Number 1, in an interactive PDF format.

Is your gas chromatography system functioning as well as it could be? Are you taking advantage of the latest innovations in this analytical technique? In this ebook, experts explore troubleshooting tools and resources for GC users.

I’m frustrated with static headspace sampling!

Looking back, the start of my penning of blog articles matches quite well with the start of efforts to investigate the potential environmental impacts of unconventional oil and gas extraction, and the formation of the Collaborative Laboratories for Environmental Analysis and Remediation (CLEAR; http://clear.uta.edu) at the University of Texas Arlington. It also well coincided with my effort to begin some outside consulting activities, predominantly the review of forensics evidence for blood alcohol determination. Both of these activities have blossomed considerably in the past seven years, and it has become interesting to contemplate the most rewarding way to spend my time going forward.

Supplemental tables

Click the title above to open the LCGC North America January 2020 issue, Volume 38, Number 1, in an interactive PDF format.

We return to the important topic of buffers, this time focusing on what happens when there is a mismatch between the mobile-phase buffer pH and the pH that the sample is buffered at.

Advanced separation and mass spectrometry methods enable comprehensive profiling of the inherent glycan heterogeneities of protein therapeutics. In particular, reversed-phase HPLC–based multiattribute methods (MAMs) provide a wealth of information, and other techniques, such as HILIC and CE-MS, also continue to evolve.

Drug substance development requires a complex range of analytical methods, presenting a challenge to analytical chemists to reliably transfer and execute these fit-for-purpose methods to external manufacturing sites. Here, we provide guidelines to follow, and share examples of realworld problems and the strategies used to remedy them.

What concentration of aqueous buffer should I use in the mobile phase when developing a re-versed‑phase liquid chromatography (LC) method for the analysis of ionogenic compounds?

I have edited the “GC Connections” column in LCGC for over 32 years. In that time, gas chromatography (GC) has changed from a specialized separations technique to being more routine for standardized methods, while expanding outward to complex multidimensional separations and scaling downward towards miniaturization and portability. In my last “GC Connections” article before stepping away from the role of Editor, I give some perspective on how GC has changed and grown over the years, and where it might go in the future.

Size-exclusion chromatography (SEC) is a mainstay in the biopharmaceutical industry, serving as a gold standard analytical tool for the characterization of therapeutic proteins in development and manufacturing settings. Contemporary SEC separations can be performed using columns packed with sub-2-μm particles, and these platforms offer the highest efficiencies available for the separation of monoclonal antibody monomer species from low- and high-molecular-weight product-related impurities. Compared to other chromatographic modes used to characterize proteins, SEC is unique in that analytes are not retained by the stationary phase. As a result, special care is required to achieve in practice the chromatographic efficiency that is expected in theory. In this article, we describe the fundamental aspects of achieving high performance using sub-2-μm SEC columns. In addition, we discuss trends in the biopharmaceutical industry, including challenges that can be addressed using modern size-exclusion technologies.

The 36th International Symposium on Microscale Separations and Bioanalysis (MSB2020) will take place at the Conference Center Le Grand Large in the city of Saint‑Malo, France, from 5–8 April 2020.

A new technique, ice concentration linked with extractive stirrer (ICECLES), provides highly efficient isolation of trace compounds from aqueous samples. ICECLES combines freeze concentration with stir-bar sorptive extraction into a seamless analytical extraction methodology. This month, we discuss how to perform this relatively simple technique, and discuss its advantages and limitations.