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Chicago Bound

Chicago, Illinois, USA, will host the 71st annual Pittcon conference and exposition. Thousands of separation scientists from across the globe and from a wide spectrum of industries will gather at the McCormick Place convention centre to discuss, investigate, and assess the latest scientific advances in technology and instrumentation.

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The Eastern Analytical Symposium (EAS) Award for Outstanding Achievements in the Fields of Analytical Chemistry was presented to Joel M. Harris at EAS 2019 on Wednesday 20 November.

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LCGC is proud to announce that Daniel W. Armstrong and Szabolcs Fekete are the winners of the 13th annual LCGC Lifetime Achievement and Emerging Leader in Chromatography Awards, respectively.

I’ve been dealing recently with issues in the laboratory when using ammonium acetate buffers, including surprising rises in HPLC–MS back pressures when starting the instrument after overnight storage, as well as difficulties with MS sensitivity.

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To fully characterize a protein biopharmaceutical, it must be broken down into smaller segments (peptides). Several high performance liquid chromatography (HPLC) techniques can be used to provide a wealth of information on everything from post-translational modifications (PTMs) to the glycoprofile to information on similarity when characterizing biosimilars.

I run into scientists all the time who have never heard the term exposome. Most are not intimately connected in the analytical world; these days, analytical scientists seemingly expect “ome” and “omics” to be tacked onto pretty much anything.

Chromatographic peak integration continues to be a major regulatory issue and was first discussed in this column in 2015. Is the approach to manual intervention and manual integration outlined still acceptable in the light of regulatory citations and guidance documents published since then?

The potential of food packaging components to migrate into food is an important health concern. Perfecto Paseiro Losada and his group at the University of Santiago de Compostela, in Spain, have been investigating the migration kinetics and actual migration of such compounds into a variety of types of food. They also have been carrying out studies to estimate dietary exposure. Paseiro recently spoke to LCGC about this work.

Fundamentals aspects of using HPLC techniques for protein analysis are described as they relate to the analysis of post-translational modifications (PTMs) and glycoprofiles.

In HPLC Diagnostics Skills Part I we looked at baseline issues, and we continue here with HPLC peaks and in particular the skills required to identify tailing peaks, the causes of peak tailing, and most importantly, how to fix the issues that give rise to this peak deformation.