Biological, Medical, and Clinical Analysis

In his keynote lecture at HPLC 2023, Alejandro Cifuentes from the Laboratory of Foodomics, Institute of Food Science Research, CIAL, CSIC in Madrid, Spain, highlighted the promising application of foodomics in addressing Alzheimer’s disease (AD) (1).

A new study published in the Journal of Chromatography A aims to further characterize different classes of biomolecules through size-exclusion chromatography.

In this interview, we explore the significance of this groundbreaking technology and its impact on mass spectrometry with Aaron Robitaille, Director of Marketing for Mass Spectrometry at Thermo Fisher Scientific in San Jose, California.

Scientists at NACRW discussed using CE–MS to analyze highly polar agrochemicals.

Researchers have developed a polybutylene terephthalate-based stationary phase for ion-pair-free reversed-phase liquid chromatography.

The application of various new methods and instruments for the analysis of multispecifics is discussed as a means of highlighting their ability to facilitate the development of these important new modalities.

A study conducted by researchers at Rowan University explores column selection in compact capillary liquid chromatography (LC) systems.

Researchers have introduced a novel primary method utilizing gas chromatography–isotope dilution infrared spectrometry (GC–IDIR) for accurate peptide purity analysis.

Scientists have developed a novel sorbent, diphenyl-functionalized nickel foam (NF-2Ph), for the accurate detection of polycyclic aromatic hydrocarbon (PAH) contaminants in Chinese herbal medicines. The method, which combines dispersive solid-phase extraction (DSPE) with gas chromatography-mass spectrometry (GC–MS) analysis, offers high sensitivity and precision, providing a reliable approach for quality control and safety assessment of these medicinal products.

Scientists from the Pacific Northwest National Laboratory are using high-resolution ion mobility spectrometry-mass spectrometry to identify unknown compounds.

New research using matrix-assisted laser desorption ionization-mass spectrometry imaging (MALDI-MSI) has revealed the distinct distributions of acetaminophen and its metabolite in the kidneys at an unprecedented resolution of 10 μm. The study, conducted by Mitsutoshi Setou and his team from Hamamatsu University School of Medicine in Japan, provides valuable insights into drug distribution and metabolism, enhancing our understanding of the pharmacokinetics and potential nephrotoxicity of acetaminophen.

Researchers have discovered that phospholipids form doubly charged lipid-metal ion complexes during electrospray ionization (ESI), providing valuable insights into the analysis of phospholipid structures.

Researchers have introduced MCnebula, a new framework for untargeted LC–MS/MS data analysis. MCnebula focuses on critical chemical classes and utilizes visualization techniques to streamline the analysis process, enabling efficient pathway analysis, biomarker discovery, and classification of unknown compounds beyond the limits of spectral libraries.

A new method has been developed for detecting and quantifying polycyclic aromatic hydrocarbons (PAHs) in intermediates used in plastics production. The method, based on liquid chromatography column switching, allows for the analysis of 18 PAHs and addresses the lack of regulatory oversight for PAH concentrations in plastic intermediates.

A research team has developed a new approach for selecting precursor ions in tandem mass spectrometry (MS/MS) experiments called DiffN, which increases the likelihood of biomarker discovery and provides a promising tool for lipidomic analyses.

In Alejandro Cifuentes's keynote lecture at HPLC 2023, he discussed the research he and his team have done to explore how foodomics can be effective in combating Alzheimer's disease.

At HPLC 2023, Erin Baker held a lecture on how using liquid chromatography and mass spectrometry together can help scientists further understand diseases that can arise from chemical exposure.

In his plenary lecture at HPLC 2023, Michal Holčapek presented his team's findings on how chromatography can help scientists record the human lipodome and better our understanding of diseases.

Gunda Köllensperger spoke at HPLC 2023 about how using U13C-isotopically labelled biomass extracts can change how scientists approach metabolomics.

In this HPLC 2023 keynote lecture, Mario Thevis discusses how chromatographic–mass spectrometric approaches have led to important advancements in sports drug testing programmes.

A new study reveals that real-time library searching significantly improves the structural characterization of glycerophospholipids and sphingomyelins, offering enhanced selectivity and comprehensive analysis. By utilizing real-time identifications to trigger targeted MSn analyses, researchers gain deeper insights into these crucial lipid species.

Researchers have discovered diacylglycerols as potent biomarkers for the rapid diagnosis of breast cancer, using mass spectrometry imaging. This breakthrough study offers new possibilities for improved intraoperative assessment and personalized treatment strategies.

Mixed-mode cationic exchange (MCX) sorptive tapes combined with direct infusion mass spectrometry enable selective extraction and rapid analysis of cationic compounds in complex samples, such as opioids in saliva.

The development of a newly-designed Girard's reagent, HBP, as charged isobaric mass tags has enabled selective capture and sensitive detection of aldehyde metabolites. The LC–dNLS-MS/MS screening approach using this reagent has successfully facilitated the discovery of natural aldehydes and their profiling in complex samples.

Researchers have developed an innovative strategy for compound annotation in offline 2D-LC–MS analysis. The approach combines hand-in-hand alignment with targeted molecular networking (TMN) and was successfully applied to analyze the chemical profile of Yupingfeng (YPF), a traditional Chinese medicine prescription, resulting in the identification of 497 compounds and demonstrating its efficiency and scalability in complex sample analysis.

A newly developed UHPLC–MS/MS method allows for the accurate analysis of bioactive compounds, including apigenin, apigenin 7-glucoside, and chlorogenic acid, in goat serum.

This article explores what this transition to newer technology looks like, with an example applied to determining organic impurities of pramipexole dihydrochloride.

Scientists achieve a breakthrough in separation techniques with the development of alizarin-modified iron oxide magnetic nanoparticles, enabling highly efficient and selective separation of anthraquinones from complex plant extracts.

The latest developments and challenges of using liquid chromatography (LC) for copolymer analysis are discussed.

Superficially porous particle (SPP) column technology is leveraged to optimize high-throughput HPLC methods for small-molecule drug discovery, resulting in improved efficiency and reduced runtime.