Application Notes: General

Flow rate is a common method parameter for most adsorptive LC methods. However, SEC is unique in that lower flow rates often yield improvements in separation, and resolution can improve considerably simply by running at a lower linear velocity. This application note demonstrates this approach for the aggregate analysis of NIST mAb, a common standard protein.

Assessing phosphate concentration may be necessary to ensure that no differences are observed with modulating concentration. This ensures proper sample recovery and demonstrates robustness of the method. In this application note, we review SEC chromatographic performance for trastuzumab and NIST mAb modulating concentration of potassium phosphate.

In this application note we explore the use of sub-2-µm particles for SEC methods as a practical way to increase throughput for applications that demand it.

Size exclusion chromatography, or SEC, is a technique for separating proteins and other complex biomolecules in their native forms by size using aqueous eluents. For molecules of specific molecular weight, SEC can use used to detect and quantitate aggregates, fragments, monomers and dimer. This guide provides a comprehensive application overview for Biozen dSEC Size Exclusion Columns.

In this application note, we demonstrate the effect of organic solvent on SEC analysis of an ADC “mimic.” This sample is a monoclonal antibody that has been conjugated to a dansyl group and functions as a surrogate for the typically cytotoxic agent commonly used for most ADC modalities.

In this application note, we demonstrate the use of SEC-HRMS for the characterization of NIST mAb RM 8671, a commonly used standard monoclonal antibody surrogate for method development.

In this application note, we demonstrate the use of SEC-HRMS for the analysis of an IdeZ-digested mAb.

Size exclusion chromatography, or SEC, is a technique for separating proteins and other complex biomolecules in their native forms by size using aqueous eluents. For molecules of specific molecular weight, SEC can use used to detect and quantitate aggregates, fragments, monomers and dimer. This guide provides an overview of the Biozen SEC Size Exclusion Chromatography Columns for the analysis of monoclonal antibodies, biosimilars and other biomolecules

Erythromycin analysis in creams, lotions, etc., based on HPAEC-PAD using the ALEXYS Antibiotics Analyzer according to the official 2018 USP 41—NF36 monograph.

SMX and TMP are the active ingredients of Co-trimoxazole or Bactrim. They can be analyzed easily and inexpensively using HPLC with pulsed amperometric detection.

The ALEXYS HPAEC-PAD Analyzer is used for the analysis of both antibiotics, in accordance with the USP 38 monographs.

A HPAEC-PAD method is presented for the sensitive analysis of mono-, di-, and trisaccharides in plant tissue extracts and root exudates..

This application note demonstrates a fast, selective, and sensitive method for the analysis of lactose and isomers based on HPAEC with Pulsed Amperometric Detection.

A HPAEC-PAD method with a four-step potential waveform is applied for the analysis of Erythritol, Xylitol, Sorbitol, Mannitol, and Maltitol, resulting in excellent reproducibility.

The ALEXYS Antibiotics Analyzer is used for routine assay and impurity analysis of Azithromycin in various formulations according USP 41 monographs.

Multi-angle light scattering couples inline with ion exchange chromatography to estimate the aggregate content of protein fractions without additional offline assays.

timsTOF technology coupled with advances in ion delivery are providing unprecedented sensitivity, realizing unbiased single-cell proteomics.

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This tech note demonstrates the benefits of single quadrupole mass detection for small protein analysis.

Quantitative recollection of split sample to a second sample saver tube is demonstrated for different split ratios on the CDS 7550S automated thermal desorber.