Application Notes: General

Routine, high-throughput laboratories must balance analytical speed, sensitivity, uptime, and reproducibility while managing demanding quantitative workflows. This Instrument Insights explores how the Thermo Scientific™ TSQ Certis Triple Quadrupole Mass Spectrometer (MS) is designed to address these challenges. Ready-to-run workflows and compliance-ready software support can simplify method development and routine operation across clinical research, toxicology, food safety, environmental, and biopharma applications. In addition, performance data demonstrates the robustness in complex matrices and long analytical sequences.

This digital supplement explores the analytical hurdles laboratories face as PFAS testing pushes into trace-level territory, from a chemical universe spanning millions of structures to persistent background contamination and non-harmonized methods across regulatory bodies. Featured research covers non-targeted screening strategies for identifying unsuspected PFAS in complex food matrices, advanced multisorbent extraction and mass spectrometry workflows for organofluorines in municipal wastewater, and findings that PFAS concentrations in estuaries can shift four- to sevenfold within a single tidal cycle. Additional coverage details the development of fluorinated SPME sorbents for selective extraction of volatile PFAS and a fast GC-MS/MS method capable of detecting 37 PFAS compounds in food packaging in just 8 minutes. Together, these articles offer a data-driven look at emerging tools and techniques designed to keep pace with PFAS's expanding chemical scope and evolving regulatory demands.

This executive summary examines how field-flow fractionation coupled with multi-angle light scattering (FFF-MALS) characterizes lipid nanoparticles (LNPs) as regulatory expectations from FDA, EMA, and USP converge on a shared set of critical quality attributes. It walks through how FFF-MALS resolves particle size, molar mass, and payload distribution in a single non-destructive run, and includes a case study comparing two authorized mRNA vaccines. Readers will see how size-resolved analysis uncovered differences in payload distribution that bulk-average methods missed.

This custom eBook examines how advanced analytical characterization is changing biosimilar development following the FDA's October 2025 draft guidance. It covers modern SEC separations for biosimilar antibodies, LC-MS and MAM workflows for protein characterization and attribute monitoring, and the role of multi-angle light scattering (MALS) in biosimilar approval. A closing Q&A with Matthew A. Lauber, PhD, of Waters Corporation, explores how integrating these complementary techniques builds a more complete, regulatory-ready picture of biosimilarity.

The use of Electronic nose (e-nose) systems to detect, classify, and in some cases quantify volatile organic compounds (VOCs) and other gases is increasing. An e-nose generally combines one or more chemical sensors to produce a characteristic response for a gas sample. E-nose systems have been investigated for applications including food quality, environmental monitoring, healthcare diagnostics, industrial monitoring, and security. A recent review describes e-noses as systems incorporating sensor arrays with controlled gas flow, data processing, and algorithms that help recognize and classify a distinctive gas mixture [1]. Although sensor technology continues to advance, the performance of an e-nose is dependent on the quality and precision of the chemical gas standards used to develop and evaluate it. Sensors must be calibrated with a known gas composition and concentration before their response is meaningful. Other important factors such as sampling techniques, differences in sensor technologies and materials, data processing, and changes in environmental conditions should be considered during calibration when analyzing e-nose performance.

This LCGC International wallchart eBook provides a comprehensive, flowchart-based reference for sample preparation techniques used in analytical chemistry. It opens with a main decision-tree flow chart guiding users from raw sample to injection-ready extract, then branches into detailed, step-by-step workflows for six major extraction methods: classical liquid-liquid extraction, solid-liquid extraction, solid-phase extraction (SPE), modern Soxhlet extraction, QuEChERS extraction (for fruits, vegetables, and other food matrices), and supercritical fluid extraction. Each section walks through decision points—such as solvent selection, particle size, recovery acceptability, and troubleshooting steps like breaking emulsions or adjusting extraction conditions—to help separation scientists choose and optimize the right approach for their sample type. Supporting reference tables (e.g., solvent pairs for LLE, SPE mechanisms and phases, QuEChERS reagent formulations) are embedded throughout to aid practical decision-making, making this a quick-access clinical/lab reference tool for method selection and troubleshooting.

Move beyond inferred measurements and gain direct insight into the viral vector quality attributes that drive efficacy, safety, and manufacturing confidence.

See how CDMS accurately characterizes AAV reference materials and generates robust data to support development, comparability, and QC workflows.

Directly distinguish empty, partial, full, and overfilled capsids while detecting additional impurities in a single analysis.

Discover how direct mass measurement reveals viral vector heterogeneity and provides a more complete understanding of capsid populations and product quality.

Simultaneously measure aggregates, capsid titer, genome titer, molecular weight, size, and empty/full ratio from a single analysis.

Monitor AAV enrichment as it happens to improve process understanding, increase consistency, and reduce manufacturing risk.

This supplement explores sustainability, data analysis, and instrumentation advances shaping modern gas chromatography (GC). Articles examine carrier gas life-cycle impacts, interpretable clustering methods for complex GC-mass spectrometry (GC–MS) datasets, polyethylene glycol (PEG) column performance, and the fundamentals of bench-top GC–tandem mass spectrometry (MS/MS). Additional coverage includes a two-dimensional GC symposium review and interviews on advanced methods for detecting PFAS and micro/nanoplastics, highlighting GC's expanding role in environmental analysis.

This supplement explores sustainability, data analysis, and instrumentation advances shaping modern gas chromatography (GC). Articles examine carrier gas life-cycle impacts, interpretable clustering methods for complex GC-mass spectrometry (GC–MS) datasets, polyethylene glycol (PEG) column performance, and the fundamentals of bench-top GC–tandem mass spectrometry (MS/MS). Additional coverage includes a two-dimensional GC symposium review and interviews on advanced methods for detecting PFAS and micro/nanoplastics, highlighting GC's expanding role in environmental analysis.

See how mycotoxin sample preparation in peanuts can be faster and more efficient with manual or automated workflows, high recoveries and time saving.

GC-FID enables fast, reproducible analysis of 37 FAMEs in edible oils, improving fatty acid profiling for quality, authenticity, and adulteration detection.

GC-FID enables accurate benzene analysis in USP Carbomer 940, meeting system suitability and supporting compliant pharmaceutical quality control workflows.

Analyze residual solvents in flexible food packaging with GC 2400 and HS 2400 M, delivering accurate, compliant results and no carryover.

Effluent wastewater testing is essential for protecting human health and the environment, and EPA Method 624.1 is a widely used approach for monitoring volatile organic compounds (VOCs) in water. This application note demonstrates how the combination of the Teledyne LABS Tekmar Lumin Purge & Trap (P&T) concentrator, AQUATek LVA autosampler, and PerkinElmer GCMS 2400 platform delivers accurate, reliable, and highly sensitive VOC analysis while effectively managing water vapor transfer that can compromise GC/MS performance.

Dibenzyl disulfide (DBDS) is a corrosive sulfur compound that can accelerate copper sulfide formation in transformers, increasing the risk of insulation damage and equipment failure. Routine monitoring of DBDS is therefore critical for transformer health, regulatory compliance, and preventive maintenance. This application note demonstrates how the PerkinElmer GC 2400 System with Electron Capture Detection (ECD) provides a sensitive, reliable, and cost-effective approach for DBDS analysis in transformer oils, delivering excellent linearity, precision, and freedom from carryover in accordance with IEC 62697 guidelines.

Selecting the right LC system is a strategic decision. The right choice depends on your samples, purification goals, detection needs, throughput, workflow complexity, available lab space, and plans for future growth. BUCHI’s Liquid Chromatography Buyer’s Guide helps you evaluate these factors clearly, so you can choose your next system with confidence. Inside the guide: Five steps to identify your purification pathway Guidance on matching system capability to workflow needs Considerations for flash chromatography and prep HPLC Decision tree to help determine which system is the best fit The guide introduces two BUCHI chromatography system families: Pure Essential: designed for basic flash applications and first steps into automated chromatography. Pure Excellence: BUCHI’s flagship liquid chromatography system family for high-performance purification.

BUCHI continues to drive innovation in chromatography. To help you unlock brilliance in your lab, BUCHI launched the Pure Excellence Chromatography System, a new flash (and optional prep HPLC) instrument designed for ease of use, advanced precision, and enhanced safety. Curious to know how you can improve high-performance purifications? Join BUCHI specialists, Alina Gau and Birke Götz, as they showcase how the new Pure Excellence Chromatography System simplifies your pharma and chemistry R&D workflows.

The product portfolio includes the C-905, C-910, and C-915 for flash applications, offering flexible detection options, including ELSD. The C-950 further expands application capabilities by integrating prep HPLC, providing added versatility for laboratories with advanced purification requirements.Building on BUCHI’s long-standing reputation for reliability and innovation, the Pure Excellence Chromatography Systems combine user-friendly operation with high separation performance. Key features include: A quick-release cartridge holder enabling secure, one-handed cartridge installation to simplify daily operation. Automated and manual sample injection modes supporting both liquid injection and solid sample loading for improved reproducibility and time efficiency. A high-performance pump with a three-piston design ensures pulse-free flow. Evaporative Light Scattering Detection (ELSD) offers near-maintenance-free operation and high detection accuracy through patented direct nano-pulse injection technology. Four-solvent gradient capability expands method development flexibility and simplifies challenging separations. Advanced safety features, including continuous system monitoring, active ventilation with a closed fraction collector (enabling operation outside a fume hood), and RFID-based fraction collector rack identification. Intuitive software with method/run preview, simplified gradient editing, and always visible run monitoring supports faster, more consistent purification. With a focus on simplicity, performance, and safety, the Pure Excellence Chromatography Systems enable laboratories to achieve purification goals faster and with greater confidence.

See how the Pure Excellence Chromatography System makes purification faster, safer, and easier than ever. In this product demo, we walk through every step of running a chromatographic separation, from priming the solvent lines to collecting your final fractions.

This study investigates the separation of egg white proteins using flash and prep HPLC with silica stationary phases varying in pore and particle sizes. Larger pore sizes improved protein interaction with the stationary phase, while particles above 10 µm were ineffective. Optimal separation was achieved using the Pure Excellence C-950 with a PrepPure C18WP column, combining wide pores, small particles, and a step gradient.

Comprehensive characterization of biotherapeutics requires advanced analytical strategies capable of detecting and identifying low-abundant product quality attributes (PQAs), including post-translational modifications, aggregates, sequence variants, and conjugation sites. This eBook presents three technical articles demonstrating advanced LC-MS workflows that achieve up to 10X improvement in MS sensitivity for trace-level detection and confident identification of these challenging attributes.

Discover a next generation laboratory balance designed for connected, automated environments. This article explains how network ready weighing technology supports regulatory compliance, digital workflows, and seamless integration into robotics and lab IT systems, enabling smarter data handling and more efficient, reproducible laboratory processes.

This white paper summarizes harmonized pharmacopeial requirements for analytical balances across USP, Ph.Eur., JP, and ChP. It explains regulatory timelines, calibration and performance check expectations, environmental control recommendations, and practical guidance for meeting accuracy and repeatability criteria in pharmaceutical quality control laboratories.