Application Notes: LC

A straightforward separation of 10 closely related forms of vitamin E with a Kinetex 2.6 µm F5 HPLC column and under reversed-phase conditions. The 10 closely related structures were chromatographically separated by the multiple selectivity interaction mechanisms of the Kinetex F5 phase and Water/Methanol mobile phase.

This method presents the reversed-phase retention and detection of 7 polar Neonicotinoids and 4 deuterated-internal standards by LC-MS/MS and with a Kinetex 2.6 Biphenyl HPLC column. The Kinetex Biphenyl phase demonstrated significant polar compound retention and selectivity.

Described in this application note are the details for an investigation of basic compound peak shape across four different alkyl C18 stationary phases that are all based upon the same core-shell HPLC/UHPLC particle morphology.

In this application, is the investigation into the relative selectivity of four different C18 stationary phases when applied to the reversed phase retention of six extremely polar artificial sweeteners.

For this analysis, we developed a fast, robust, and efficient separation on a Kinetex 2.6µm Polar C18 phase. This is a highly robust, core-shell stationary phase capable of providing increased efficiency over fully porous products. The Kinetex Polar C18 functionality provides unique polar selectivity that was critical in effectively retaining and separating these phenolic antioxidants.

For Developed is a rapid method analyzing 16 major cannabinoids with great resolution and low backpressures.

A straightforward separation of 10 closely related forms of vitamin E with a Kinetex 2.6 µm F5 HPLC column and under reversed-phase conditions. The 10 closely related structures were chromatographically separated by the multiple selectivity interaction mechanisms of the Kinetex F5 phase and Water/Methanol mobile phase.

For this application study, the alkaloid compound berberine was selected to help demonstrate the Kinetex PS C18 HPLC/UHPLC column’s unique multi-modal selectivity and improved chromatographic performance when applied to the analysis of polar basic compounds.

For this application study, the alkaloid compound berberine was selected to help demonstrate the Kinetex PS C18 HPLC/UHPLC column’s unique multi-modal selectivity and improved chromatographic performance when applied to the analysis of polar basic compounds.

This application is an alternative HPLC method for the analysis of 13 carbonyls without the use of any derivatization agent. The method uses the combined polar and non-polar selectivity of a Kinetex Biphenyl stationary phase to achieve improved chromatographic results.

For this analysis, we developed a fast, robust, and efficient separation on a Kinetex 2.6µm Polar C18 phase. This is a highly robust, core-shell stationary phase capable of providing increased efficiency over fully porous products. The Kinetex Polar C18 functionality provides unique polar selectivity that was critical in effectively retaining and separating these phenolic antioxidants.

This application is an alternative HPLC method for the analysis of 13 carbonyls without the use of any derivatization agent. The method uses the combined polar and non-polar selectivity of a Kinetex Biphenyl stationary phase to achieve improved chromatographic results.

This method presents the reversed-phase retention and detection of 7 polar Neonicotinoids and 4 deuterated-internal standards by LC-MS/MS and with a Kinetex 2.6 Biphenyl HPLC column. The Kinetex Biphenyl phase demonstrated significant polar compound retention and selectivity.

For Developed is a rapid method analyzing 16 major cannabinoids with great resolution and low backpressures.

A HPLC/UHPLC poster that ranks different stationary phases by 5 primary mechanism of reversed-phase selectivity.

Described in this application note are the details for an investigation of basic compound peak shape across four different alkyl C18 stationary phases that are all based upon the same core-shell HPLC/UHPLC particle morphology.

In this application, is the investigation into the relative selectivity of four different C18 stationary phases when applied to the reversed phase retention of six extremely polar artificial sweeteners.

Analysis of paralytic shellfish toxins using a HPLC single step-gradient method with products of post-column derivatization detected using a fluorescence detector

The advantage of UV spectroscopy as an analytical method to detect protein aggregation is that it is non-destructive and uses low sample volumes, minimal sample preparation requirement and easy sample analysis. A quick QC method to indicate the presence of aggregation uses an Agilent Cary 60 UV-Vis spectrophotometer to identify aggregation of mAb solutions resulting from different stress conditions.

Decrease analysis time for fluorochemicals with no sacrifice in chromatographic performance. Rapid perfluorinated alkyl analysis by LC-MS/MS increases sample throughput.

Decrease analysis time for fluorochemicals with no sacrifice in chromatographic performance. Rapid perfluorinated alkyl analysis by LC-MS/MS increases sample throughput.

The Agilent 1290 Infinity II UHPLC, an Agilent 6545 Q-TOF-MS, and Agilent MassHunter BioConfirm B.08.00 software was used for accurate disulphide bond mapping.

An isocratic HPLC method showing the separation of an artificial sweetener in a cola beverage. The chromatogram shows the advantage of using 230nm instead of 254nm for improved detection but minimizing the baseline interferences.

With the increase of research with Eudragit, consistent molecular weight measurements have become more prevalent. Shodex has shown a repeatable SEC method using the GF-510 HQ column.

TSKgel® UP-SW3000 columns are 2 µm SEC columns designed for the analysis of monoclonal antibodies and other biopharma products and can be used on both HPLC and UHPLC systems. A TSKgel UP-SW3000 column yielded very low percent relative standard deviation (%RSD) for peak parameters including retention times, peak asymmetry, and efficiency, demonstrating the exceptional reproducibility of this column versus a competitor UHPLC column.

The characterization of monoclonal antibodies is a major challenge in process monitoring and quality control. TSKgel® G3000SWXL columns have been the industry standard for quality control of mAbs by SEC for decades. With the introduction of TSKgel UP-SW3000, 2 µm silica-based UHPLC–HPLC columns, increased speed and higher resolution can be achieved for the separation of antibody fragments, monomers, and dimers.

This application note discusses the use of a 3 μm particle size, 30 nm pore size, size exclusion chromatography (SEC) column for monitoring folded (native) and unfolded (denatured) states of a monoclonal antibody using fluorescence detection (FLD).

Successful separations of the enantiomers of 3,3’-substituted BINOLs and their corresponding protected derivatives were obtained on the RegisPack® chiral stationary phase (CSP) from Regis Technologies, Inc.