Application Notes: LC

TSKgel® UP-SW3000 columns are 2 µm SEC columns designed for the analysis of monoclonal antibodies and other biopharma products and can be used on both HPLC and UHPLC systems. A TSKgel UP-SW3000 column yielded very low percent relative standard deviation (%RSD) for peak parameters including retention times, peak asymmetry, and efficiency, demonstrating the exceptional reproducibility of this column versus a competitor UHPLC column.

The characterization of monoclonal antibodies is a major challenge in process monitoring and quality control. TSKgel® G3000SWXL columns have been the industry standard for quality control of mAbs by SEC for decades. With the introduction of TSKgel UP-SW3000, 2 µm silica-based UHPLC–HPLC columns, increased speed and higher resolution can be achieved for the separation of antibody fragments, monomers, and dimers.

This application note discusses the use of a 3 μm particle size, 30 nm pore size, size exclusion chromatography (SEC) column for monitoring folded (native) and unfolded (denatured) states of a monoclonal antibody using fluorescence detection (FLD).

Successful separations of the enantiomers of 3,3’-substituted BINOLs and their corresponding protected derivatives were obtained on the RegisPack® chiral stationary phase (CSP) from Regis Technologies, Inc.

This Application Note proves equivalency of the Agilent 1200 Series LC and the Agilent 1260 Infinity II LC for the analysis of tricyclic antidepressant drugs.

This Application Note proves equivalency of the Agilent 1200 Series LC and the Agilent 1260 Infinity II LC for the analysis of tricyclic antidepressant drugs.

Analysis of arsenic species is made challenging due to diverse sample matrices. To circumvent these problems, an HPLC ICP-MS method has been developed. Resolution of arsenic compounds is achieved by ion exchange chromatography on a Hamilton PRP-X100 column, a 55% cross-linked polystyrene-divinylbenzene copolymer functionalized with quaternary ammonium anion-exchanger group.

Pickering Laboratories, Inc. offers a complete solution for Amino Acids Analysis according to European Pharmacopoeia 8.0. This includes the Pinnacle PCX post-column derivatization instru¬ment, analytical columns and GARDs, buffers and Trione Ninhydrin reagent. The methods presented in this application note were optimized to comply with system suitability requirements of Pharmacopoeia 8.0 methods.

Pickering Laboratories, Inc. offers a complete solution for Amino Acids Analysis according to European Pharmacopoeia 8.0. This includes the Pinnacle PCX post-column derivatization instru¬ment, analytical columns and GARDs, buffers and Trione Ninhydrin reagent. The methods presented in this application note were optimized to comply with system suitability requirements of Pharmacopoeia 8.0 methods.

Monitoring of Glyphosate in crops and water is mandated in many countries. Pickering offers a sensitive and robust HPLC method for analysis of Glyphosate in soy beans, corn and sunflower seeds. This method utilizes a simplified sample preparation procedure that has proven to be effective even for challenging matrices.

A CHIRALPAK IG column (immobilized meta selector) was used to develop the enantioselective separation of methylclothiazide. Meta-substituted immobilized chiral selectors have been shown to have remarkable affinity for resolution of chiral compounds from different types of molecules. Available in 3 and 5 micron.

A CHIRALPAK IG column (immobilized meta selector) was used to develop the enantioselective separation of methylclothiazide. Meta-substituted immobilized chiral selectors have been shown to have remarkable affinity for resolution of chiral compounds from different types of molecules. Available in 3 and 5 micron.

The quick capture and accurate titer analysis over a wide concentration range of monoclonal antibody is demonstrated using a TSKgel® Protein A-5PW, 20 μm analytical column.

The Agilent 1290 Infinity II LC was used to determine the bitter compounds iso-alpha-acids and reduced iso-alpha-acids, and the clove-like phenolic flavor 4-vinylguaiacol in bottled beer.

The Agilent 1290 Infinity II LC was used to determine the bitter compounds iso-alpha-acids and reduced iso-alpha-acids, and the clove-like phenolic flavor 4-vinylguaiacol in bottled beer.

The Agilent 1290 Infinity II UHPLC, an Agilent 6545 Q-TOF/MS, and Agilent MassHunter BioConfirm B.08.00 software was used for accurate disulfide bond mapping.

The Agilent 1290 Infinity II UHPLC, an Agilent 6545 Q-TOF-MS, and Agilent MassHunter BioConfirm B.08.00 software was used for accurate disulphide bond mapping.

A CHIRALPAK IG column (immobilized meta selector) was used to develop the enantioselective separation of methylclothiazide. Meta-substituted immobilized chiral selectors have been shown to have remarkable affinity for resolution of chiral compounds from different types of molecules.

A CHIRALPAK IG column (immobilized meta selector) was used to develop the enantioselective separation of methylclothiazide. Meta-substituted immobilized chiral selectors have been shown to have remarkable affinity for resolution of chiral compounds from different types of molecules.

This Application Note proves equivalency of the Agilent 1100 Series Quaternary LC and the Agilent 1260 Infinity II LC for the analysis of antihistaminic drugs.

This Application Note proves equivalency of the Agilent 1100 Series Quaternary LC and the Agilent 1260 Infinity II LC for the analysis of antihistaminic drugs.

exposure to MOHs (Mineral Oil Hydrocarbons) via packaging could contribute significantly to the total exposure to these contaminants and may pose a human health hazard. In the present work, migration of MOHs and DIPN from recycled paperboard boxes in direct contact with dry pasta (both semolina and egg pasta) has been monitored during shelf life at regular times (up to two years).

Analysis of additives such as sweeteners, preservatives, and caffeine in various food products, beverages, and consumer toothpaste using the Agilent 1290 Infinity II LC.

Analysis of additives such as sweeteners, preservatives, and caffeine in various food products, beverages, and consumer toothpaste using the Agilent 1290 Infinity II LC.

A 12-minute analytical method was developed for 122 VDs in meat. The method uses Agilent All Ions MS/MS on a Q-TOF LC/MS instrument along with the Agilent Veterinary Drugs PCDL.

PAHs were determined in a petroleum vacuum distillation residue using the Agilent 1290 Infinity Multiple Heart-Cutting 2D-LC solution with normal-phase in the first dimension and reversed-phase in the second dimension.

PAHs were determined in a petroleum vacuum distillation residue using the Agilent 1290 Infinity Multiple Heart-Cutting 2D-LC solution with normal-phase in the first dimension and reversed-phase in the second dimension.

In this application the Agilent 1290 Infinity II LC was used to determine the bitter compounds iso-alpha-acids and reduced iso-alpha-acids, and the clove-like phenolic flavor 4-vinylguaiacol in bottled beer.