Application Notes: LC

The Agilent 1290 Infinity II UHPLC, an Agilent 6545 Q-TOF-MS, and Agilent MassHunter BioConfirm B.08.00 software was used for accurate disulphide bond mapping.

A CHIRALPAK IG column (immobilized meta selector) was used to develop the enantioselective separation of methylclothiazide. Meta-substituted immobilized chiral selectors have been shown to have remarkable affinity for resolution of chiral compounds from different types of molecules.

A CHIRALPAK IG column (immobilized meta selector) was used to develop the enantioselective separation of methylclothiazide. Meta-substituted immobilized chiral selectors have been shown to have remarkable affinity for resolution of chiral compounds from different types of molecules.

This Application Note proves equivalency of the Agilent 1100 Series Quaternary LC and the Agilent 1260 Infinity II LC for the analysis of antihistaminic drugs.

This Application Note proves equivalency of the Agilent 1100 Series Quaternary LC and the Agilent 1260 Infinity II LC for the analysis of antihistaminic drugs.

exposure to MOHs (Mineral Oil Hydrocarbons) via packaging could contribute significantly to the total exposure to these contaminants and may pose a human health hazard. In the present work, migration of MOHs and DIPN from recycled paperboard boxes in direct contact with dry pasta (both semolina and egg pasta) has been monitored during shelf life at regular times (up to two years).

Analysis of additives such as sweeteners, preservatives, and caffeine in various food products, beverages, and consumer toothpaste using the Agilent 1290 Infinity II LC.

Analysis of additives such as sweeteners, preservatives, and caffeine in various food products, beverages, and consumer toothpaste using the Agilent 1290 Infinity II LC.

A 12-minute analytical method was developed for 122 VDs in meat. The method uses Agilent All Ions MS/MS on a Q-TOF LC/MS instrument along with the Agilent Veterinary Drugs PCDL.

PAHs were determined in a petroleum vacuum distillation residue using the Agilent 1290 Infinity Multiple Heart-Cutting 2D-LC solution with normal-phase in the first dimension and reversed-phase in the second dimension.

In this application the Agilent 1290 Infinity II LC was used to determine the bitter compounds iso-alpha-acids and reduced iso-alpha-acids, and the clove-like phenolic flavor 4-vinylguaiacol in bottled beer.

In this application the Agilent 1290 Infinity II LC was used to determine the bitter compounds iso-alpha-acids and reduced iso-alpha-acids, and the clove-like phenolic flavor 4-vinylguaiacol in bottled beer.

This application note describes the use of ISOLUTE SLE+ for extraction of estrone (E1) and estradiol (E2) from human serum. Analysis is performed by an HPLC-MS/MS method that gives limits of detection of 0.001 ng/mL without derivatization or fluoride adduct stabilization. ISOLUTE SLE+ products provides high analyte recoveries, no emulsion formation and significantly reduced sample preparation time compared to traditional liquid-liquid extraction.

This application note describes a UHPLC/MS/MS-based method for the screening and quantification of more than 250 pesticides and pesticide metabolites in food samples.

This application note describes a UHPLC/MS/MS-based method for the screening and quantification of more than 250 pesticides and pesticide metabolites in food samples.

A CHIRALPAK IG column (immobilized meta selector) was used to develop the enantioselective separation of methylclothiazide. Meta-substituted immobilized chiral selectors have been shown to have remarkable affinity for resolution of chiral compounds from different types of molecules.

This Application Note describes the method transfer of a USP HPLC method for amlodipine besylate tablets to a UHPLC method using the Agilent 1290 Infinity II LC.

This Application Note describes the method transfer of a USP HPLC method for amlodipine besylate tablets to a UHPLC method using the Agilent 1290 Infinity II LC.

11-nor-9-Carboxy-THC, also known as THCA or carboxy-THC, is the main secondary metabolite of THC (the active component of marijuana) formed in the human body [1]. THCA is excreted in urine in the form of glucuronide conjugates. THCA is not psychoactive but has a long half-life of up to several days or even weeks in very heavy users, thus determination of THCA in urine plays an important role in confirmation of marijuana consumption. The Substance Abuse and Mental Health Services Administration (SAMHSA) has set the THCA cutoff concentration of confirmatory testing at 15 ng/mL. Typical sample preparation methods for THCA in urine include liquid-liquid extraction (LLE) and solid phase extraction (SPE). This application utilizes a novel sample preparation technique, QuEChERS to effectively quantitate THCA levels in human urine.

Using a targeted solid phase extraction (SPE) method and GC–ECD analysis, chlorinated pesticides are extracted from poultry fat resulting in a method that decreases the amount of labor and reagents when compared to the USDA-FSIS CHC2 method.

A simple, automatable cleanup method is developed for the analysis of acrylamide from coffee, yielding absolute recoveries that exceed 92% when analyzed by LC–MS-MS.

Using a targeted solid phase extraction (SPE) method and GC–ECD analysis, chlorinated pesticides are extracted from poultry fat resulting in a method that decreases the amount of labor and reagents when compared to the USDA-FSIS CHC2 method.

A simple, automatable cleanup method is developed for the analysis of acrylamide from coffee, yielding absolute recoveries that exceed 92% when analyzed by LC–MS-MS.

Cannabinoids are successfully extracted from a complex brownie matrix using the QuEChERS sample preparation technique, significantly reducing the presence of matrix interferences. The resulting clean samples are analyzed by GC–MS using a specialized deactivated GC column to provide heightened peaks for the cannabinoid compounds.

EPA Method 625 is greatly simplified by using solid phase extraction (SPE) as an alternative to traditional liquid-liquid extraction (LLE). Using this SPE technique, paired with a sensitive GC–MS analysis, increases throughput and data quality, while decreasing manual labor and solvent usage.

Cannabinoids are successfully extracted from a complex brownie matrix using the QuEChERS sample preparation technique, significantly reducing the presence of matrix interferences. The resulting clean samples are analyzed by GC–MS using a specialized deactivated GC column to provide heightened peaks for the cannabinoid compounds.

Improving and optimizing EPA Method 593 using solid phase extraction (SPE) enhances sensitivity and maximizes efficiency while screening by LC–MS-MS for different female birth control hormones present in drinking and waste water.

EPA Method 625 is greatly simplified by using solid phase extraction (SPE) as an alternative to traditional liquid-liquid extraction (LLE). Using this SPE technique, paired with a sensitive GC–MS analysis, increases throughput and data quality, while decreasing manual labor and solvent usage.