Application Notes: LC

This Application Note demonstrate ultrafast gradients with an over 60 % reduction of analysis time using the Agilent 1290 Infinity II Multisampler with dual-needle function and alternating column regeneration.

This Application Note demonstrate ultrafast gradients with an over 60 % reduction of analysis time using the Agilent 1290 Infinity II Multisampler with dual-needle function and alternating column regeneration.

This application note describes techniques to increase sample throughput without compromising the accuracy of analysis during the monitoring of aggregate formation during biotherapeutic development

This application note describes techniques to increase sample throughput without compromising the accuracy of analysis during the monitoring of aggregate formation during biotherapeutic development

Previously, SFE and SFC were offline operations for pretreatment or analysis, respectively, and treated as completely separate workflows. This note, however, presents a flow diagram showing the integration of SFE-SFC processes from pretreatment to data acquisition in a single workflow, as well as an analysis of reduced coenzyme Q10.

This note describes the use of TSKgel UP-SW3000, 2µm SEC columns for the analysis of proteins, with data demonstrating the operation of these columns using a simple and well established method for use in both HPLC and UHPLC systems. TSKgel UP-SW3000 columns have superior resolution for proteins and the shorter column dimension, 4.6 mm ID × 15 cm, allows runs to be completed 2 times faster than its longer column dimension counterpart without compromising resolution and reproducibility.

This Application Note demonstrates the sensitive and rapid determination of Polycyclic Aromatic Hydrocarbons (PAHs) in tap water using the Agilent 1200 Infinity Series Online SPE Solution.

This Application Note demonstrates the sensitive and rapid determination of Polycyclic Aromatic Hydrocarbons (PAHs) in tap water using the Agilent 1200 Infinity Series Online SPE Solution.

Centrifugal Partition Chromatography (CPC) also known as Counter Current Chromatography (CCC) is a preparative, pilot and industrial liquid purification technique that does not require traditional solid supports. CPC was used to purify few mg of gingerol from crude extract.

This Application Note shows the transfer of a standard HPLC method to a UHPLC method for the analysis of 13 DNPH-derivatized aldehydes and ketones. By transferring the method from HPLC to UHPLC, the analysis time and solvent consumption was reduced by approximately 90 %.

This Application Note shows the transfer of a standard HPLC method to a UHPLC method for the analysis of 13 DNPH-derivatized aldehydes and ketones. By transferring the method from HPLC to UHPLC, the analysis time and solvent consumption was reduced by approximately 90 %.

This Application Note describes UHPLC methods to simultaneously separate and identify seven tannin compounds as aging markers in barrel-aged alcoholic beverages. The proposed methodology was applied to four different matrixes: whisky, brandy, rum, and tequila, to demonstrate a correlation between aging time and total tannin concentration level.

This Application Note shows the easy transfer of an LC method from the Waters Alliance 2695 LC to an Agilent 1290 Infinity II LC, with ISET, while controlled through Waters Empower software. Paracetamol and its impurities were analyzed on both systems, and the resulting retention times and resolution values were compared.

This Application Note shows the easy transfer of an LC method from the Waters Alliance 2695 LC to an Agilent 1290 Infinity II LC, with ISET, while controlled through Waters Empower software. Paracetamol and its impurities were analyzed on both systems, and the resulting retention times and resolution values were compared.

Agilent Intelligent System Emulation technology (ISET) in the Agilent 1290 Infinity II LC facilitates the transfer of methods from conventional HPLC systems such as the Agilent 1100 Series Binary LC. This Application Note illustrates the advantages of ISET for the analysis of six tricyclic antidepressants.

Agilent Intelligent System Emulation technology (ISET) in the Agilent 1290 Infinity II LC facilitates the transfer of methods from conventional HPLC systems such as the Agilent 1100 Series Binary LC. This Application Note illustrates the advantages of ISET for the analysis of six tricyclic antidepressants.

This Application Note shows the transfer of an analysis method for anti-epileptic drugs from HPLC to UHPLC using the Agilent 1290 Infinity II LC system. An enormous time and solvent saving of approximately 95 % was achieved with an UHPLC separation optimized for speed.

The Agilent 1290 Infinity II High Dynamic Range Diode Array Detection Impurity Analyzer Solution combines the signals from two diode array detectors with different path length Max-Light cartridge cells to increase the linear dynamic UV-range. This solution can be used to detect and quantify main and trace compounds in a single run without exceeding the linear UV-range.

This Application Note shows the transfer of an analysis method for anti-epileptic drugs from HPLC to UHPLC using the Agilent 1290 Infinity II LC system. An enormous time and solvent saving of approximately 95 % was achieved with an UHPLC separation optimized for speed.

Ultrahigh throughput is shown for ultrafast gradients with over 60 % reduction of total analysis time using alternating column regeneration. This was accomplished with parallel injection by the second needle installed in the Agilent 1290 Infinity II Multisampler, which has a dual-needle option for the analysis of food preservatives.

Ultrahigh throughput is shown for ultrafast gradients with over 60 % reduction of total analysis time using alternating column regeneration. This was accomplished with parallel injection by the second needle installed in the Agilent 1290 Infinity II Multisampler, which has a dual-needle option for the analysis of food preservatives.

The Agilent 1290 Infinity II High Dynamic Range Diode Array Detection Impurity Analyzer Solution combines the signals from two diode array detectors with different path length Max-Light cartridge cells to increase the linear dynamic UV-range. This solution can be used to detect and quantify main and trace compounds in a single run without exceeding the linear UV-range.

"This Application Note shows the analysis of 20 explosives with the Agilent 1290 Infinity II Variable Wavelength Detector (VWD) in dual-wavelength mode using 254 and 214 nm."

This Application Note describes the use of the Agilent 1290 Infinity II LC for the automated operation of seven different applications using different methods in food analysis. The exchange of columns is controlled by the method used. All columns are located in a single Agilent 1290 Infinity II Multicolumn Thermostat. Accordingly, the Agilent 1290 Infinity II Flexible Pump is attached to two external solvent-selection valves for automated solvent switching.

Evaporative light scattering (ELS) is a unique and highly sensitive technique for detection of semivolatile and nonvolatile solutes in a liquid stream. As a consequence of the physics of light scattering, conventional ELS detectors have a relatively small dynamic range. In contrast, the Agilent 1290 Infinity II Evaporative Light Scattering Detector has a 10-times higher dynamic range compared to conventional ELS detectors.

This Application Note describes the use of the Agilent 1290 Infinity II LC for the automated operation of seven different applications using different methods in food analysis. The exchange of columns is controlled by the method used. All columns are located in a single Agilent 1290 Infinity II Multicolumn Thermostat. Accordingly, the Agilent 1290 Infinity II Flexible Pump is attached to two external solvent-selection valves for automated solvent switching.

Evaporative light scattering (ELS) is a unique and highly sensitive technique for detection of semivolatile and nonvolatile solutes in a liquid stream. As a consequence of the physics of light scattering, conventional ELS detectors have a relatively small dynamic range. In contrast, the Agilent 1290 Infinity II Evaporative Light Scattering Detector has a 10-times higher dynamic range compared to conventional ELS detectors.

"This Application Note shows the analysis of 20 explosives with the Agilent 1290 Infinity II Variable Wavelength Detector (VWD) in dual-wavelength mode using 254 and 214 nm."