John Chasse

In the final segment of our three-part interview with Jon Bardsley of Thermo Fisher, Jon discusses mitigation strategies and emerging tools to consider when testing for PFAS.

A study conducted by the College of Veterinary Medicine of Midwestern University (Glendale, Arizona), the University of California Davis School of Veterinary Medicine (Davis, California) and the College of Veterinary Medicine of the University of Tennessee (Knoxville, Tennessee) set out to describe the pharmacokinetics of oral gabapentin (GABA) in goats given as a single dose (SD) and multidose (MD) regimen, as well as to document any adverse effects after administration. Plasma samples were collected and analyzed via reversed-phase high performance liquid chromatography (RP-HPLC).

A joint study between the Nursing School of North Henan Medical University (Xinxaing, China) and The First Affiliated Hospital of Harbin Medical University (Harbin, China) used gas chromatography–mass spectrometry (GC-MS) for an observational study collecting and classifying surgical smoke generated during laparoscopic surgery to evaluate the carcinogenic risks to healthcare workers.

A recent review conducted by the Food Science Departments of Cairo University (Giza, Egypt), the University of Basrah (Basrah, Iraq) and Damascus University (Damascus, Syria) examined the wide array of bioactive compounds present in plant-based foods, with emphasis on their extraction, isolation, identification, characteristics, and emerging applications. These compounds were separated, purified, and analyzed from their complex plant-based matrices through a variety of chromatographic and spectrometric techniques.

Jon Bardsley of Thermo Fisher offers his perspective regarding the recent advances in PFAS detection in pharmaceutical manufacturing through the utilization of liquid chromatography-mass spectrometry (LC-MS), and factors that should be considered in achieving even greater advances.

Researchers at the University of Akron (Akron, Ohio) and Slippery Rock University (Slippery Rock, Pennsylvania) used gas chromatography-mass spectrometry (GC-MS) to analyze nicotine-free, cinnamon-flavored e-cigarette vapor to identify chemicals that might impact zebrafish cardiovascular development.

Researchers at the College of Life Science at Northwest Normal University (Lanzhou, China) used gas chromatography-ion mobility spectrometry (GC-IMS) to analyze the volatile organic compounds (VOCs) of olive oil extracted from eight maturity indices of Koroneiki olive fruits to determine the optimal harvest period.

Gas chromatography-mass spectrometry (GC-MS) was used in a joint study between the David Geffen School of Medicine at the University of California Los Angeles (Los Angeles, California) and the Department of Pediatrics at Boston Children's Hospital (Boston, Massachusetts) comparing parenteral nutrition–associated cholestasis, growth, and fatty acids in infants with gastrointestinal (GI) disorders who received either multioil intravenous lipid emulsion (MO ILE) or soybean oil lipid emulsion (SO ILE).

Jon Bardsley of Thermo Fisher offers his perspective regarding the advancement of PFAS detection in pharmaceutical manufacturing with liquid chromatography-mass spectrometry (LC-MS), and the influence of regulations in the pharmaceutical and biopharmaceutical industries.

Research conducted at Uskudar University (Istanbul, Turkey) explored the neuroprotective potential of ethanolic extracts of celery leaves, specifically in the treatment of Alzheimer’s disease. Liquid chromatography (LC) and mass spectrometry (MS)-based metabolomics analysis of the extract revealed the existence of a diverse array of secondary metabolites, including phenolic acids, hydroxycinnamic acid, flavonoids, flavonoid O-glycosides, flavonol, glycosides, and isoflavones.

A recent study aiming to investigate, for the first time, the biochemical and nutritional composition of the peels from five purple and two red potato cultivars and evaluate them as sources of healthy ingredients for the formulations of nutraceuticals quantified total phenolic content, carbohydrates, proteins, and lipids using UV–visible (UV-vis) spectrophotometry. The phytochemical composition was further characterized via high-performance liquid chromatography coupled with a diode-array detector (HPLC-DAD).

A joint study conducted by KU Leuven (Leuven, Belgium) and Wollo University (Dese, Ethiopia) aimed to develop and validate a liquid chromatography method with ultraviolet detection (LC-UV) for the identity screening and assay of 13 different injectable antibiotics in pharmaceutical formulations.

Researchers at the University of Otago (Dunedin, New Zealand) and the University of Auckland (Auckland, New Zealand) out to show that applying audible sound via a linear actuator, which impacted primarily on particle motion rather than the pressure component of audible sound, would significantly decrease beer fermentation time compared to control fermentations by keeping more viable yeast in suspension. Gas chromatography-mass spectrometry (GC-MS) was used to measure the abundance of volatile organic compounds (VOCs) in the beer samples.

Researchers at the University of Porto (Porto, Portugal) aiming to uncover novel therapeutic targets for bone loss of functionality and enhancing the understanding of its underlying bioenergetic mechanisms used liquid chromatography-mass spectrometry (LC-MS) to characterize proteomes.

Treated wastewater from three expedition North Atlantic Ocean cruise ships was collected and analyzed with a focus on pharmaceuticals, personal care products, and industrial chemicals. Samples were screened using data-dependent acquisition using liquid chromatography-high-resolution mass spectrometry (LC-HRMS).

A joint study conducted by the Henan University of Technology (Zhengzhou, China) and the Food Laboratory of Zhongyuan (Henan Province, China) developed and evaluated what the researchers deemed a novel zwitterionic stationary phase, Sil-DBO-PS, for hydrophilic interaction liquid chromatography (HILIC).LCGC International spoke to Ashraf Ali of the Henan University of Technology about this research and their findings.

Gas chromatography–mass spectrometry (GC–MS) was used to detect the chemical components of natural musk, harvested from forest musk deer at multiple stages of maturity. This research combines metabolomic profiling with biosynthetic pathway analysis to identify potential precursors and enzymes involved in musk biosynthesis.

Researchers at Baylor College of Medicine’s Children's Nutrition Research Center (Houston, Texas) developed and validated a high-throughput extraction and ultrahigh-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) method to separate and quantify 39 spinach flavonoid species in 11.5 min.

Cell membrane chromatography (CMC) technology was used by researchers in screening efficacy and antibacterial ability of articaine (AT) derivatives in the pre-emptive treatment of oral infections.

Two proof-of-concept hydrophilic interaction chromatography HILIC) methods were developed in a joint study conducted by the University of Geneva (Switzerland), Sanofi, and the Waters Corporation—one for disrupting LNPs and retaining the mRNA, and another for detecting only unencapsulated mRNA to assess encapsulation efficiency. LCGC International spoke to Jonathan Maurer, first author of the paper that resulted from this study, about the methods and the efforts that led up to their development.

Researchers at Andes Ag, Inc. (Alameda, California) established a high-throughput framework for the robust and precise quantification of soil inorganic carbon (SIC) from agricultural soils using gas chromatography (GC).

A joint study between the University of Tennessee (Knoxville, Tennessee) and the University of Pennsylvania School of Veterinary Medicine (Philadelphia, Pennsylvania) compared directly measured vitamin K1 (vitK1) concentrations in healthy dogs and dogs with chronic enteropathy (CE) using liquid chromatography tandem mass spectrometry (LC–MS/MS); they also investigated whether supplementation of vitK1 in dogs with CE would significantly increase vitK1 concentrations.

A study conducted by the Agriculture Research Centre of Giza, Egypt, and Jilin Agricultural University in China, evaluated the efficacy of stinging nettle extract, nettle smoke, and formic acid in the controlling of Varroa mites, a major threat to honeybee colonies, with a focus on mite infestation reduction, honeybee mortality, and biochemical responses. Gas chromatography–mass spectrometry (GC–MS) was used to identify key bioactive compounds in the stinging nettle extract.

An Australian study used pyrolysis coupled to gas chromatography-mass spectrometry (Pyr-GC–MS) to analyze the presence of plastic polymers in commercial and homemade composts. LCGC International spoke to Simran Kaur—a PhD candidate at the Queensland Alliance for Environmental Health Sciences (QAEHS) at The University of Queensland in Woolloongabba, Australia—to find out more about her team’s findings.

Based on their hypothesis that smelling anxiety chemosignals can, like visual anxiety induction, lead to an increase in odor sensitivity, a joint study between the University of Erlangen-Nuremberg (Erlangen, Germany) and the Fraunhofer Institute for Process Engineering and Packaging (Freising, Germany) combined behavioral experiments, odor profile analysis by a trained panel, and instrumental analysis of odorants (gas chromatography-olfactometry) and volatiles (gas chromatography-mass spectrometry).

A pilot study investigating whether nicotinamide adenine dinucleotide kinase (NADK) expression is selectively diminished in alcohol-associated liver cirrhosis (AC), as well as evaluating its potential as a biomarker for this condition, measured AC and non-AC (NAC). Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) levels in human liver samples were measured using liquid chromatography-mass spectrometry (LC-MS).

A joint study between the University of Oklahoma Health Sciences Center (Oklahoma City, Oklahoma) and the UC Davis West Coast Metabolomics Center (Davis, California) identified differentially regulated lipids in type 2 diabetes (T2D) and obesity through the application of reversed-phase liquid chromatography-accurate mass tandem mass spectrometry (RP-LC-accurate MS/MS).

Saba Aslani from the University of Texas at Arlington spoke to LCGC International about a collaborative project with Northwestern University, the University of Hong Kong, and BioTools, Inc., investigating mirror-image cyclodextrins using ultra-high performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) and vibrational circular dichroism (VCD).

A novel method called quantitative marinating (QM) was developed to reduce industrial waste during the processing of crayfish meat, with the taste, flavor, and aroma of crayfish meat processed by various techniques investigated. Headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS) was used to determine volatile compounds of meat examined.

Researchers developing a plant microbial consortium that can repair in-situ high concentration TNT (1434 mg/kg) contaminated soil, as well as overcome the limitations of previous studies that only focused on simulated pollution, used untargeted metabolone gas chromatography-mass spectrometry (GC-MS) to measure their success.