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July / August 2023 | LCGC Europe
Enhancing Peptide Mapping Sequence Coverage Through an Automated Dual Protease Digest
August 01, 2023
Peer Reviewed
36
07
Trypsin is one of the most commonly used proteases in peptide mapping protocols because of its high level of specificity. However, trypsin alone is not always sufficient for full sequence coverage. In this article, the authors detail how trypsin was combined with chymotrypsin to overcome this, and the benefits of an automated platform.
Initiating Method Development with Scouting Gradients—Where to Begin, and How to Proceed?
August 01, 2023
LC Troubleshooting
36
07
How to design scouting gradients, how to use the resulting data to make decisions about next steps, and how to improve the separation once an elution mode has been chosen.
GC Connections: Basic Care and Feeding of Your Detector
August 01, 2023
GC Connections
36
07
This instalment explores the challenges involved in detecting the effluent from a capillary gas chromatographic column and summarizes the many detector options.
Polysorbate Analysis in Biopharmaceutical Applications—A Snapshot of the Analytical Toolbox
August 01, 2023
Biopharmaceutical Perspectives
36
07
An overview of different approaches for the qualitative and quantitative analysis of polysorbates.
A Practical Understanding of Partition Coefficients
August 01, 2023
Sample Prep Perspectives
36
07
On a fundamental level, equilibrium and diffusion define chemical separations.
Events: ISSS 2023
August 01, 2023
Events
36
07
ISSS 2023 and other important events for chromatographers.