Size-exclusion chromatography is an important technique in biopharmaceutical characterization. This article discusses its use for soluble aggregation analysis and quantitation.
Some attributes of large molecules make them behave differently from small molecules in reversed-phase separations.
The variation in selectivity of aromatic hydrocarbons with bisubstituted polar groups is investigated in systems consisting of C18 stationary phase and ternary eluents. The solutions of the ternary eluents were obtained by mixing binary solvents (organic modifier + water) of similar eluent strength. Acetonitrile, methanol and tetrahydrofuran were the organic modifiers applied. The influence of the organic solvent type and its concentration in the ternary mobile phase on retention and selectivity of the solutes is discussed. An approach previously presented by our group was adapted to explain the selectivity changes.
Part 1 of our annual report on new products introduced at Pittcon. This month, columns are highlighted, covering HPLC/UHPLC columns, packings, and related products.
Analytica 2014 Tuesday Afternoon Sessions
Analytica 2014 Tuesday Morning Sessions
Click the title above to open the LCGC Europe April 2014 regular issue, Vol 27 No 4, in an interactive PDF format.
Click the title above to open the LCGC North America April 2014 Recent Developments in LC Column Technology Supplement, Vol 32 No s4, in an interactive PDF format.
Click the title above to open the LCGC North America April 2014 regular issue, Vol 32 No 4, in an interactive PDF format.
Nick Snow of Seton Hall University studied the presence of drugs of abuse in various complex matrices to detect trace quantities of drugs of abuse using GCxGC–TOF–MS as his method of choice.
Thermo Fisher Scientific (California, USA) and Harvard Medical School (Boston, USA) have teamed up to establish the Thermo Fisher Scientific Center for Multiplexed Proteomics at Harvard Medical School. The facility has been set up for the development of new methods for protein quantitation, as well as the development of training courses to make expertise available to the wider community.
The ACS Women Chemists Committee (WCC) has awarded a 2014 WCC Rising Star Award to Katherine Ayers, Director of Research for Proton OnSite (Connecticut, USA). The award recognizes exceptional early to mid-career women chemists from across all areas of chemistry on a national level.
The latest newsletter from March 27.
The ACS Women Chemists Committee (WCC) has awarded a 2014 WCC Rising Star Award to Katherine Ayers, Director of Research for Proton OnSite (Connecticut, USA). The award recognizes exceptional early to mid-career women chemists from across all areas of chemistry on a national level.
The latest newsletter from March 27.
Click here to view the complete E-Separation Solutions newsletter from March 25, 2014.
Four new ozone-depleting substances (ODSs) - CFC–1121, CFC–112a, CFC–113a, and HCF–133a - have been detected and identified in the Earth?s atmosphere using gas chromatography?mass spectrometry (GC–MS).1 The research published in Nature Geoscience compared samples collected between 1978 and 2012 to establish a clear increase in the emissions of these gases, even though CFC production has been banned globally since 1987.
Of late we have been running a series called 'Lock and Leave', which serves as a reminder to pay attention to those instrument parameters which are often overlooked - you know the type of thing ? 'that's the best value for this system?, 'we always set to that value' and 'I have no idea what that does' ? it's always at that value'.
Silicone wristbands promoting charitable organizations and which are popular throughout the world as a fashion statement can be used to monitor exposure of individuals to contaminants in their local environment, according to a study published in the journal Environmental Science and Technology.1 The authors of the study propose silicone bands as passive samplers that can give a temporal and spatial picture of human contaminant exposure.
Silicone wristbands promoting charitable organizations and which are popular throughout the world as a fashion statement can be used to monitor exposure of individuals to contaminants in their local environment, according to a study published in the journal Environmental Science and Technology.1 The authors of the study propose silicone bands as passive samplers that can give a temporal and spatial picture of human contaminant exposure.
The National University of Singapore (Singapore) is leading a research consortium to develop the world?s first lipid database for different racial and ethnic groups, supported by Agilent Technologies (California, USA). When completed, the project will provide a centralized resource for researchers studying fat levels as diagnostic markers.
Silicone wristbands promoting charitable organizations and which are popular throughout the world as a fashion statement can be used to monitor exposure of individuals to contaminants in their local environment, according to a study published in the journal Environmental Science and Technology.1 The authors of the study propose silicone bands as passive samplers that can give a temporal and spatial picture of human contaminant exposure.
Incognito discusses the impending technological singularity of analytical chemistry. Is it time to take back control from the machine?
Scientists from the University of Florence in Italy have developed a novel fast ion chromatographic (FIC) method for the analysis of fluoride in Antarctic snow and ice. Data from fluoride analysis has the potential to assist scientists to build up a picture of past volcanic activity.
Click the title above to open The Column March 20, 2014 North American issue, Volume 10, Number 5, in an interactive PDF format.
Click the title above to open The Column March 20, 2014 Europe & Asia issue, Volume 10, Number 5, in an interactive PDF format.
Gerard Rozing has been announced as the winner of the second annual Uwe D. Neue Award in Separation Science. The award was created by Waters Corporation (Massachusetts, USA) to recognize the work of distinguished industrial scientists, in honour of Dr Uwe D. Neue, late scientist and Waters Corporate Fellow. Rozing will receive the award at the upcoming 41st International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC2014) held on 11–15 May in New Orleans, USA.
The United States woefully underfunds international collaborations with the Czech Republic. Yet, it is a country that produces excellent scientists who are distinctly passionate about science, and who have been educated to demonstrate not only a strong mastery of chemistry basics, but also superb knowledge of the integration of high-level mathematics and computer programming skills into their science. One need not look very far in the separation science and mass spectrometry (MS) fields in the United States to find eminent leaders with roots in the Czech Republic.
Click here to view the complete E-Separation Solutions newsletter from March 13, 2014.