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A simple dansylation metabolite assay (DMA) for sample normalization that is similar to the bicinchoninic acid (BCA) protein assay widely used in proteomics has been presented in the journal Analytical Chemistry. The DMA assay is performed following sample extraction to determine the total metabolite concentration, which can then be used to determine the volume of sample needed to ensure that all samples contain the same concentration of metabolites.

Click the title above to open The Column October 24, 2014 North American issue, Volume 10, Number 19, in an interactive PDF format.

Click the title above to open The Column October 24, 2014 Europe & Asia issue, Volume 10, Number 19, in an interactive PDF format.

A team of researchers at the University of Huddersfield, UK, has investigated the potential of okra for emulsification of certain food products using size-exclusion chromatography (SEC) and fourier-transform infrared (FT-IR) spectroscopy.

Click the title above to open The Column October 05, 2014 Europe & Asia issue, Volume 10, Number 18, in an interactive PDF format.

The LCGC Europe news update from October 16 2014.

Click here to view the complete E-separation Solutions newsletter from October 16, 2014.

Why would one want to use a mobile phase containing 100% water? Usually to wash the analyte or old mobile phase from the column (stationary phase surface) or to help retain analytes which elute quickly using mobile phases containing only very small amounts of organic solvent (usually highly polar analytes).

The LCGC Europe news update from October 9 2014.

Unconventional oil and gas (UOG) extraction, which includes processes such as horizontal drilling and hydraulic fracturing, has created major positive geopolitical and economic advantages for the United States.

The development of a column-switching technique based on on-line preconcentration and HRAM–MS to obtain fast and accurate results for the determination of algal toxins in drinking water is discussed.

Researchers from the Institute of Food Chemistry at Westfälische Wilhelms‑Universität Münster in Germany have developed an optimized high performance liquid chromatography coupled with mass spectrometry (HPLC–MS–MS) method using multiple reaction mode (MRM) and MRM3 for detecting horse and pork in highly processed food.

Scientists from CEITEC Masaryk University are collaborating with clinicians from the Department of Respiratory Diseases and TB at the University Hospital Brno (Brno, Czech Republic) to improve the diagnosis of cystic fibrosis, specifically in newborn babies. In a new study published in the Journal of Chromatography A, a new "skin wipe" sweat sampling method is presented that reduces the time required for sampling to under a minute and utilizes capillary electrophoresis (CE) to simultaneously determine chloride, sodium, and potassium ions in sweat samples.

Liquid chromatography coupled to triple quadrupole tandem mass spectrometry (LC–MS–MS) in multiple reaction monitoring (MRM) mode is widely considered as the gold standard for pesticide analysis. In this article recent developments in LC–MS–MS technology to meet increasing regulatory demands and productivity targets in quality control and contract laboratories are explored.

This article provides some tips and tricks for monitoring sample recovery and peak area using GPC/SEC.

To understand how the HPLC system works, it can be useful to first identify the basic components of virtually every system. Armed with that information, envisioning the HPLC system as comprised of three different zones - each with unique characteristics and each with its own set of requirements - can offer further guidance.

Click the title above to open The Column October 05, 2014 North American issue, Volume 10, Number 18, in an interactive PDF format.

Agilent Technologies

Agilent Technologies

Wyatt Technology Corporation

Click here to view the complete E-separation Solutions newsletter from October 2, 2014.

Malvern Instruments

Tosoh Bioscience GmbH

Biotage GB Ltd

Restek Corporation

Agilent Technologies

Akzo Nobel

Bruker Daltonics