The goals in ultrahigh-pressure liquid chromatography (UHPLC) method development are to first find the best separation, second find the best column, and third find the most robust method in a multifactorial Design Space. Trial and error methods are not sufficient anymore and solid science based on Quality by Design (QbD) principles is required.
Click the title above to open The Column April 08, 2014 North American issue, Volume 10, Number 6, in an interactive PDF format.
Click the title above to open The Column April 08, 2014 Europe & Asia issue, Volume 10, Number 6, in an interactive PDF format.
In two new videos from LCGC TV, Milton L. Lee from Brigham Young University discusses the principles of TGGC, and the possible advantages of TGGC over traditional GC methods.
Scientists from Bristol-Myers Squibb Company have developed a novel approach for the simultaneous quantitation of neutralizing antibodies, residual drug, and human Immunoglobulin G (IgG) in immunogenicity assay development using liquid chromatography?tandem mass spectrometry (LC–MS–MS). This work published in Analytical Chemistry presents a new approach that aids the refinement of therapeutic protein neutralizing antibody assays in drug development.
Click here to view the complete E-Separation Solutions newsletter from April 3, 2014.
Analytica 2014 Thursday Morning Sessions
The latest newsletter from April 3.
Analytica 2014 Thursday Afternoon Sessions
Analytica 2014 Wednesday Afternoon Sessions
Analytica 2014 Wednesday Morning Sessions
Chromatography has taken a prominent place in the characterization and analysis of protein therapeutic drugs and today it plays a critical role in the biotechnology laboratory.
Gerard Rozing has been announced as the winner of the second annual Uwe D. Neue Award in Separation Science.
An examination of the development of new types of columns based on different particle types, sizes, and other physical characteristics and how they can improve the speed and efficiency of HPLC used to support more expansive and complicated analyses
LCE December 2013 BPA
LCGC NA December 2013 BPA
The winners of LCGC's seventh annual awards are highlighted.
New product introductions in the areas of HPLC columns for reversed-phase LC, HILIC, ion-exchange and ion chromatography, SEC, and specialty columns for specific types of analyses are covered.
A brief introduction to the articles presented in this supplement
Why do core–shell particles provide an increase in efficiency?
Size-exclusion chromatography is an important technique in biopharmaceutical characterization. This article discusses its use for soluble aggregation analysis and quantitation.
Some attributes of large molecules make them behave differently from small molecules in reversed-phase separations.
The variation in selectivity of aromatic hydrocarbons with bisubstituted polar groups is investigated in systems consisting of C18 stationary phase and ternary eluents. The solutions of the ternary eluents were obtained by mixing binary solvents (organic modifier + water) of similar eluent strength. Acetonitrile, methanol and tetrahydrofuran were the organic modifiers applied. The influence of the organic solvent type and its concentration in the ternary mobile phase on retention and selectivity of the solutes is discussed. An approach previously presented by our group was adapted to explain the selectivity changes.
Part 1 of our annual report on new products introduced at Pittcon. This month, columns are highlighted, covering HPLC/UHPLC columns, packings, and related products.
Analytica 2014 Tuesday Afternoon Sessions
Analytica 2014 Tuesday Morning Sessions
Click the title above to open the LCGC Europe April 2014 regular issue, Vol 27 No 4, in an interactive PDF format.
Click the title above to open the LCGC North America April 2014 Recent Developments in LC Column Technology Supplement, Vol 32 No s4, in an interactive PDF format.
Click the title above to open the LCGC North America April 2014 regular issue, Vol 32 No 4, in an interactive PDF format.