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A column classification system for SFC to help with method development has been developed. This article demonstrates how the system can be used to select a column when developing a new SFC method.

Analytica 2014 Conference Spotlight

Certain considerations must be made with regards to the overall ?ow path used in a SFC analysis to ensure additional obstacles are not introduced inadvertently. This article provides more information.

Analytica 2014 Guide to Munich

Analytica 2014 Exhibitor Highlights

Scientists in Malaysia have developed a useful metabolomics approach that could aid analysts working in natural drug discovery. The team applied liquid chromatography–mass spectrometry (LC–MS) coupled with principle component analysis (PCA) to screen more than 4000 natural extracts for novel photosensitizer drugs.

Click the title above to open The Column March 10, 2014 North American issue, Volume 10, Number 4, in an interactive PDF format.

Click the title above to open The Column March 10, 2014 Europe & Asia issue, Volume 10, Number 4, in an interactive PDF format.

The latest newsletter from March 6.

LC–MS Deconstructs the "Poppy Seed Defense"

Gerstel GmbH & Co. KG (M?lheim an der Ruhr, Germany) and Spark Holland (Emmen, The Netherlands) have announced an original equipment manufacturer (OEM) agreement. The agreement will allow Gerstel to develop and market fully integrated automated dried blood spot (DBS) technology from Spark Holland that recently received a US patent.

Frederic Lynen discusses why LC–MS is the ideal analytical tool for the quantitative analysis of analytes in biological samples, and presents a new HILIC-based SPE approach to improve analyte recovery and sensitivity.

Wyatt Technology Corporation

Click the title above to open the LCGC Europe March 2014 Analytica Guide Supplement, Vol 27 No s3, in an interactive PDF format.

Event: ISC 2014: The Sound of Chromatography

An efficient method for forensic analysis of amphetamines and synthetic cathinones - the illicit drugs often called "bathsalts" - in hair samples

The advent of ultrahigh-pressure liquid chromatography (UHPLC) and its successful commercialization in the last few years has brought forth a modern high performance liquid chromatography (HPLC) platform capable of higher speed, resolution, precision, and sensitivity. Currently, all major HPLC manufacturers have some type of low-dispersion UHPLC products with upper pressure limits ranging from 15,000 to 19,000 psi (1000 to 1300 bar) on the market. This installment describes a number of popular myths or half-truths in UHPLC and provides data that contradict or even repudiate some of these commonly held beliefs.

Daniel W. Armstrong, a professor of chemistry and biochemistry at the University of Texas Arlington in Arlington, Texas, is to receive the 2014 American Chemical Society Award in Separations Science and Technology.

In the pharmaceutical industry, the demonstration of drug substance (DS) or drug product (DP) stability over the shelf life is an important aspect of the regulatory requirements.

A look at some highlights for chromatographers at Analytica 2014.

If scaling isocratic separations is so simple, why is gradient scaling so confusing?

Stacking and sweeping injection methods are compared and practical suggestions are provided about how to enhance sensitivity for charged and neutral compounds.

This instalment discusses the storage and use of carrier and detector gases, gas filtration, and the means by which pressures are controlled on the way from the gas supplies to a GC instrument.

What good is that big, ugly peak at the beginning of the chromatogram?

Currently available methods for the detection of matrix effects in liquid chromatography–mass spectrometry (LC–MS) are tedious and complex; therefore, a simpler method is required. Although there are no methods to completely eliminate matrix effects, the most well-recognized technique available to correct for matrix effects is that of internal standardization using stable isotope–labelled versions of the analytes. As this method can prove expensive, an alternative method of correction is likely to be useful. In this study, a simple method based on recovery is assessed for the detection of matrix effects. Two alternative methods for the rectification of matrix effects in LC–MS are also assessed: Standard addition and the coeluting internal standard method.

The mechanisms of mixed-mode chromatography are discussed with reference to the parameters that are used to optimize selectivity and retention. Illustrative examples are presented with strategies for column selection and screening to allow this technique to be quickly and effectively adopted by end users.

There has been a significant resurgence in the development of antibody-drug conjugates (ADC) as target-directed therapeutic agents for cancer treatment.

Click the title above to open the LCGC Asia Pacific March 2014 regular issue, Vol 17, No 1, in an interactive PDF format.