All News

Estimating Uncertainty

E-Separation Solutions

Estimating uncertainty has become one of the most important metrological concepts in analytical science over the last 15 years to such an extent that some authors consider a result useless or invalid unless it is accompanied with an uncertainty statement. This article describes how to estimate uncertainty in chromatographic analysis and how laboratories can calculate it using data from the method validation process.

TechForum_f-569682-1408672775479.jpg

E-Separation Solutions

This month E-Separations Solutions' Technology Forum looks at the topic of capillary electrophoresis and the trends and issues surrounding it. Joining us for this discussion is Tobias Preckel of Agilent Technologies; Susan Darling of Beckman Coulter, Inc.; and Frank A. Gomez of California State University.

E-Separation Solutions

The answer to the following reader question about resolution loss in a GC analysis was supplied by LCGC's "GC Connections" columnist John Hinshaw.

The Column: December 2008

The Column

Do Wrinkle Creams Really Work? High Performance Thin-Layer Chromatography for the Analysis of Lipids Valeria Widmer and Eike Reich UHPLC for the Analysis of Paracetamol Anke Knöfel and Silvia Marten News All the news from December 2008 Incognito Perfecting HPLC Method Specifications As a follow on to last month's column, Incognito provides some tips on how to properly specify HPLC methods as a result of his continual experience of coming across methods that fall short of the standards required Market Trends and Analysis Market Profile: Flash Chromatography Glenn Cudiamat Tips and Trick: GPC/SEC A Development in High Temperature (HT) GPC/SEC Peter Montag Q&A Animal Pharma Professor Chris Shaw from Queens University, Belfast, UK discusses the techniques used to study biologically-active peptides from reptile and amphibian venom in drug discovery

i8_t-588318-1408653701277.jpg

LCGC Europe

Cytosine (chemical name 4-amino-2-hydroxypyrimidine) is a pyrimidine derivative with a hetereocyclic aromatic ring and two substituents (amine and keto groups) attached and is a polar compound of significant biological and pharmaceutical interest. In response to the intended use of bulk cytosine as a raw material in pharmaceutical manufacturing, a method for the determination of the purity of cytosine was developed.

i4-588317-1408653704589.jpg

Estimating Uncertainty

LCGC Europe

Estimating uncertainty has become one of the most important metrological concepts in analytical science over the last 15 years to such an extent that some authors consider a result useless or invalid unless it is accompanied with an uncertainty statement. This article describes how to estimate uncertainty in chromatographic analysis and how laboratories can calculate it using data from the method validation process.

i3_t-572715-1408670302629.jpg

Digital Update

LCGC Europe

Careful synchronization is essential when performing sample preparation and chromatographic analysis in parallel for optimized productivity. Automated sample preparation requires reliable and compatible hardware combined with integrated software control of the whole analysis process, including LC–MS or GC–MS analysis.

i1-572716-1408670301025.jpg

Event News

LCGC Europe

Pittcon 2009 celebrates 60 years of service to the scientific community. Pittcon 2009 will be held from 8–13 March 2009 at McCormick Place South, in Chicago, Illinois, USA. Pittcon is the biggest annual meeting on laboratory science in the world, incorporating a laboratory product exposition, a diverse, high-quality technical programme and invaluable peer-to-peer networking opportunities.

i4-572711-1408670313645.gif

LCGC Europe

Electrophoretic techniques, such as sodium dodecyl sulphate polyacrylamide (SDS-PAGE) and isoelectric focusing (IEF) gels, have traditionally been part of release testing in the biopharmaceutical industry. However, these slab gel methods are inconvenient and irreproducible because of the staining/destaining steps used in analyte detection, the use of toxic reagents and high intra- and inter-gel effective mobility variability.

Column Care

LCGC Europe

Another precaution I recommend is not to shut off the LC system for more than an hour or so if it or the column contains buffer.

i4-572713-1408670308039.jpg

LCGC Europe

Estimating uncertainty has become one of the most important metrological concepts in analytical science over the last 15 years to such an extent that some authors consider a result useless or invalid unless it is accompanied with an uncertainty statement. This article describes how to estimate uncertainty in chromatographic analysis and how laboratories can calculate it using data from the method validation process.

Researchers Edward Staples from the US measurement technology company Q&A Solutions (Westlake Village, CA) and Shekar Viswanathan from the National University (San Diego, CA) have developed a new method for detecting contraband in cargo containers using urface acoustic waves (SAWs) and a capillary-based gas chromatograph column.

Scientist Cornelia Kasper and colleagues from the Gottfried Wilhelm Leibniz University of Hanover (Hanover, Germany) thought that a liquid chromatography method with short monolithic columns would be a quicker alternative to measure proteins in culture while also having had the potential to separate different proteins.

Researchers from National Tsing Hua University (Hsinchu, Taiwan) developed a dispersive liquid?liquid microextraction (DLLME) method based on solidification of a floating organic drop and combined it with gas chromatography with either electron-capture or mass spectrometry detection.

A research group from the University of La Laguna and ICIA (both in La Laguna, Tenerife, Canary Islands, Spain) used multiwalled carbon nanotubes as a solid-phase extraction (SPE) medium for extracting eight organophosphorus pesticides from various commercial apple, grape, orange, and pineapple juices.

PerkinElmer, Inc. (Waltham, MA) announced the acquisition of Arnel Inc. (Parlin, NJ), a provider of custom engineered solutions for gas chromatography applications in the petrochemical, food and beverage, and industrial hygiene markets.

Researchers from the University of Barcelona (Barcelona, Spain) and Arboriculura Mediterranea Mas de Bover (Tarragona, Spain) implemented a factorial experimental design in developing a solid-phase microextraction (SPME)?gas chromatography (GC)?mass spectrometry (MS) method to analyze volatile phenols in virgin olive oils.