Food and Beverage Analysis

An overview of current chromatography-based food toxicant screening is presented.

Agilent Poroshell 120, a superficially porous particle column, offers resolution and speed similar to columns packed with 1.8-µm totally porous particles, without generating high back pressure.

AB SCIEX Application Note

This paper describes the use of the AB SCIEX TripleTOFTM 5600 LC–MS-MS system for targeted and nontargeted screening for pesticide residue in food samples.

Participants in this Technology Forum are Deanna Hurum of Dionex Corporation, Sue Steinike of Shimadzu Scientific Instruments, and Gerry Broski and Eric Phillips of Thermo Fisher Scientific.

Thermo Fisher Scientific has announced that its global Food Safety Response Centre in Dreieich, Germany, has developed two new analytical screening methods in response to the oil spill in the Gulf of Mexico.

Participants in this Technology Forum are Deanna Hurum of Dionex Corporation, Sue Steinike of Shimadzu Scientific Instruments, and Gerry Broski and Eric Phillips of Thermo Fisher Scientific.

Michal Godula, Food Safety Specialist from Thermo Fisher Scientific answers questions on food safety testing.

The safety of the food that our children eat is a global concern. Regulations are in place that limit the maximum level of pesticides that can be present in food meant for children, and methods to detect levels well below those limits are needed to ensure the safety of the food supply. Combining the speed and separation efficiency of ultrahigh-pressure liquid hromatography (UHPLC) with the sensitivity and selectivity of triple-quadrupole mass spectrometry (MS)-MS results in a method that can deliver ultralow quantification of pesticides in baby food, with limits of detection more than an order of magnitude below the allowed maximum levels.

Peas, beans and some other plant seeds contain proteins, called globulins, which have been shown to act as a defence against insects and other herbivores. Coffee beans contain large amounts of globulins, so scientists in Brazil studied the beans to assess their potential for warding off insects.

A method is presented for the analysis of epicatechin (EC), epigallocatechin (EGC), epigallocatechingallate (EGCG), myricetin, quercetin, keampferol and cis and trans resveratrol. An ALEXYS Analyzer is used with a binary gradient. Detection limits of 2-5 nmole/L have been obtained.

Coelutions using one dimensional chromatography are often observed in flavour and fragrance analysis.

Generic methods based upon chromatography with full-scan mass spectrometry (MS) detection are maturing.

Some of the threats to food safety and the measures in place for protection are discussed

Two-dimensional polyacrylamide gel electrophoresis reveals regulatory effects of dietary polyunsaturated fatty acid supply on protein expression in early development.

The authors use headspace SIFT-MS to target and identify volatiles in various malt aldehydes. The specificity and speed are compared to current methodology.

Large-volume Injection Coupled to Comprehensive Gas Chromatography – Time-of-Flight Mass Spectrometry for the Analysis of 3-MCPD Esters in Edible Oils

An overview of current chromatography-based food toxicant screening is presented.

Polychlorinated biphenyls (PCBs) are a class of extremely persistent industrial chemicals manufactured for use in electrical transformers, capacitors, inks, paints, pesticides, dust control or insulating fluids. Estimates have put the total global production of PCBs on the order of 1.5 million tons.

As anyone involved in the field of analytical chemistry can tell you, food safety is a topic that is garnering more and more attention, not only in separations laboratories and in technical sessions at conferences and symposia around the world, but in the most telling place of all: the mainstream media.

Liquid chromatography coupled to mass spectrometry (LC–MS) using electrospray ionization (ESI) is a powerful analytical tool for the analysis of a wide molecular weight range of polar, semivolatile, and thermally labile compounds.

Mass spectrometry plays an increasingly significant role in the analysis of residues and contaminants in food. Here we will illustrate how the combination of ultrahigh-pressure liquid chromatography (UHPLC) and high-resolution time-of-flight-mass spectrometry (TOF-MS) is used to generate a screen of veterinary drug residues in products of animal origin. The use of UHPLC–TOF-MS and dedicated, workflow directed software allows rapid screening for large numbers of residues and automated quantification of positive samples. In addition, we illustrate how the data generated using MSE acquisition mode enable critical structural information to be collected, which offers additional selectivity and confirmatory data for compound identification and facilitates elucidation of the structure of newly discovered compounds.

QuEChERS Multiresidue Pesticide Method for Wine - UCT apps note

The need for better methods for vitamin D analysis in a wide variety of foods is a good showcase for the advantages that ultrahigh-pressure liquid chromatography (UHPLC) can provide. The availability of systems that can operate as high as 1200 bar and an extensive range of UHPLC column choices provide the flexibility needed to rapidly develop fast vitamin D separations that can be adapted to meet the challenges of testing a diversity of food types

This article will demonstrate the feasibility of analysing complex mixtures without any prior sample preparation by making use of the high resolving power of both UHPLC and mass spectrometry.