LC–MS

At HPLC 2023, Oliver Fiehn of the University of California explained results gathered from a device that can be used during daily digestion.

An ultrahigh-pressure liquid chromatography–tandem mass spectrometry method (UHPLC–MS/MS) using polarity switching, preceded by solid-phase extraction (SPE), was used to quantify eight ultraviolet filter (UVF) compounds in chlorinated outdoor pools in Winnipeg.

Fresh garlic and aged garlic, both brown and black, were all analyzed for their bioactives, in an attempt to expand those compounds’ applications as food ingredients and dietary supplements.

Scientists have developed a breakthrough MDLC–MS method for in-depth characterization of charge variants in therapeutic antibodies. The innovative approach enables the detection and evaluation of rare charge variants, facilitating improved safety and efficacy in antibody development.

Organic acids, flavonoids, iridoids, and saponins were among the compounds discovered, with significant α-glucosidase inhibitory behavior in vitro that was inconsistent with substance content level.

New research emphasize the importance of identifying metabolite interference in LC–MS targeted metabolomics analysis to ensure accurate results. The study highlights the need for thorough investigation and resolution of metabolite interferences for reliable metabolite measurement in targeted metabolomics.

A research team has developed a fast and sensitive method using UHPLC–MS/MS to simultaneously detect the nematicide fluensulfone (FSF) and its two major metabolites in different agricultural soils, providing valuable insights into the environmental behavior of FSF in soil systems.

A simultaneous, single-run analysis was sought for metabolites of the central carbon metabolism pathway, which is closely related to the occurrence and development of most diseases in humans.

The development of a newly-designed Girard's reagent, HBP, as charged isobaric mass tags has enabled selective capture and sensitive detection of aldehyde metabolites. The LC–dNLS-MS/MS screening approach using this reagent has successfully facilitated the discovery of natural aldehydes and their profiling in complex samples.

Researchers have developed an innovative strategy for compound annotation in offline 2D-LC–MS analysis. The approach combines hand-in-hand alignment with targeted molecular networking (TMN) and was successfully applied to analyze the chemical profile of Yupingfeng (YPF), a traditional Chinese medicine prescription, resulting in the identification of 497 compounds and demonstrating its efficiency and scalability in complex sample analysis.

Nails were evaluated for their suitability as non-invasive biomarkers for exposure to antibiotics, even those that are commonly prescribed.

A new method for monitoring tricyclic antidepressants (TCA) in biological fluids and tissues in real time was proposed for the purposes of improving their effects or determining causes of death.

In a talk at ASMS 2023, Rebecca L. Beres of the University of North Carolina at Chapel Hill focused on two types of analysis methods: liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS) and a liquid chromatography, ion mobility spectrometry, and mass spectrometry (LC–IMS-MS) platform.

Webinar Date/Time: Thu, Jun 29, 2023 12:00 PM EDT

Characterizing lipid nanoparticles (LNPs) is a developing delivery modality in biotherapeutic analysis. We address some of the current challenges and opportunities in this field, including an examination of the most common tools used to characterize LNPs.

A novel system combining hydrogel-modified human umbilical vein endothelial cell membrane chromatography with high-performance liquid chromatography-mass spectrometry revolutionizes the screening and purification of active ingredients in herbal medicines.

A new publication introduces a groundbreaking automated system for high-throughput mass spectrometry analysis, reshaping the future of biotherapeutic characterization.

A new approach was sought for the simultaneous verification of carboxyl and aldehyde metabolites, including analytes that were previously undetectable.

A recent study has introduced comprehensive quality control procedures for large-scale plasma proteomics analyses. The research provides valuable insights into optimizing performance, enhancing reproducibility, and ensuring quantitative accuracy in proteomics investigations.

Using intact protein separation (IPS) together with traditional peptide separation (PS) enhanced detection for LC–MS/MS better than either method could do independently.

A cutting-edge technique combining droplet microfluidics and mass spectrometry revolutionizes monoclonal antibody screening, enabling rapid and high-throughput analysis while setting a new standard for efficient clone selection in biopharmaceutical development.

Automation and high-throughput MALDI mass spectrometry revolutionize peptide metabolic stability screening, allowing for rapid analysis of a large number of samples and minimizing solvent waste.

A new study introduces a comprehensive two-dimensional liquid chromatography coupled with tandem mass spectrometry (2D-LC–MS/MS) method for the analysis of 112 pesticides in corn-based products. The method exhibits high precision, lower limit of quantification values, and successful detection of trace levels of pesticides in real samples, offering promise for the analysis of complex matrices.

Eighty drugs prohibited in athletic competition were analyzed, with seven samples returning adverse findings compared to previous methods.

The eggs of the yellow-legged gull and Audouin’s gull and the blood of the greater flamingo were studied to assess their exposure to perfluoroalkyl substances (PFAS) as bioindicators of chemical pollution.