LC–MS

Characterizing lipid nanoparticles (LNPs) is a developing delivery modality in biotherapeutic analysis. We address some of the current challenges and opportunities in this field, including an examination of the most common tools used to characterize LNPs.

A novel system combining hydrogel-modified human umbilical vein endothelial cell membrane chromatography with high-performance liquid chromatography-mass spectrometry revolutionizes the screening and purification of active ingredients in herbal medicines.

A new publication introduces a groundbreaking automated system for high-throughput mass spectrometry analysis, reshaping the future of biotherapeutic characterization.

A new approach was sought for the simultaneous verification of carboxyl and aldehyde metabolites, including analytes that were previously undetectable.

A recent study has introduced comprehensive quality control procedures for large-scale plasma proteomics analyses. The research provides valuable insights into optimizing performance, enhancing reproducibility, and ensuring quantitative accuracy in proteomics investigations.

Using intact protein separation (IPS) together with traditional peptide separation (PS) enhanced detection for LC–MS/MS better than either method could do independently.

A cutting-edge technique combining droplet microfluidics and mass spectrometry revolutionizes monoclonal antibody screening, enabling rapid and high-throughput analysis while setting a new standard for efficient clone selection in biopharmaceutical development.

Automation and high-throughput MALDI mass spectrometry revolutionize peptide metabolic stability screening, allowing for rapid analysis of a large number of samples and minimizing solvent waste.

A new study introduces a comprehensive two-dimensional liquid chromatography coupled with tandem mass spectrometry (2D-LC–MS/MS) method for the analysis of 112 pesticides in corn-based products. The method exhibits high precision, lower limit of quantification values, and successful detection of trace levels of pesticides in real samples, offering promise for the analysis of complex matrices.

Eighty drugs prohibited in athletic competition were analyzed, with seven samples returning adverse findings compared to previous methods.

The eggs of the yellow-legged gull and Audouin’s gull and the blood of the greater flamingo were studied to assess their exposure to perfluoroalkyl substances (PFAS) as bioindicators of chemical pollution.

A biomarker with significant clinical and analytical potential in an enzyme present in many human diseases was determined at higher efficiency using liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS).

Researchers have developed a novel liquid chromatography–mass spectrometry method for analyzing sialic acid linkage in N-linked glycopeptides, offering insights into glycosylation patterns.

We interviewed an AI program (ChatGPT) for LCGC North America asking questions about AI and its role in various applications for separation science to include data analysis, and high performance liquid chromatography (HPLC), hydrophilic-interaction chromatography (HILIC), reversed-phase liquid chromatography (RPLC), liquid chromatography–mass spectrometry (LC–MS), gas chromatography–mass spectrometry (GC–MS), high resolution mass spectrometry (HRIM–MS), high resolution tandem mass spectrometry (HRMS/MS), and related topics.

Webinar Date/Time: Tue, Jun 13, 2023 11:30 AM EDT

New statistical framework developed by researchers at the University of California in Riverside allows confident identification of isomeric molecules by mass spectrometry, even when no unique mass-to-charge peaks are generated between the isomeric pairs.

This month we interview Juan L. Benedé from the University of Valencia in Spain, about his work developing microextraction approaches, based on the use of sorbent materials, for application to complex matrices, and his focus on the determination of disease biomarkers in biofluids.

At Pittcon 2023, LCGC spoke with Jim Grinias of Rowan University. This segment is the full-length version of the interview that was conducted at the conference.

How to enhance the sensitivity, specificity, and speed of metabolite measurement using TIMS, which in turn helps to accelerate the pace of metabolomics research.

How can low-resolution mass spectrometry (LRMS) and high-resolution (HR) MS work together to provide unambiguous results in clinical and forensic toxicology?

A new study reports the development a highly sensitive LC–MS/MS method to measure salivary melatonin levels. The method has a lower limit of the measuring interval of 0.8 pg/mL, making it the most sensitive method for quantifying melatonin in saliva.

A recent study has found that using only seven points across an LC–MS/MS peak can provide sufficient accuracy and precision for drug quantitation studies in drug discovery and development, challenging the "rule-of-thumb" of using at least 15 points.

Samples of the prefrontal cortex, striatum, and hippocampus of rats were analyzed by LC–MS to assess brain tissue response to combined consumption of ethanol and cocaine.

Webinar Date/Time: Wed, May 31, 2023 11:00 AM EDT

Webinar Date/Time: Wed, Jun 28, 2023 1:00 PM EDT

A micro-pillar array format for mapping the proteome of human stem cell-derived liver organoids using timsTOF–MS is presented.

A new RPLC–MS method can map the substitution degree and composition of β-glucose monomers of cellulose ethers (CEs), providing detailed compositional distributions and constitutional isomer distributions, thereby identifying compositional variation between bio-stable and non-bio-stable CEs and unexpected methylations in EHEC samples.

A recent trend in the design of LC instrumentation is the move towards miniaturized and portable systems.

The Chromatographic Society and the Separations Science Group of the Analytical Division of the Royal Society of Chemistry are pleased to announce Emerging Separations Technologies 2023 is to be held 24–25 May 2023, at RSC Burlington House, London, UK.

A rapid and robust LC–MS/MS method for determining lactulose and rhamnose concentrations in blood plasma was used to determine intestinal permeability from blood plasma, which can help diagnose gastrointestinal diseases such as Crohn’s disease.