LC–MS

Pepsin digestion and guanidine hydrochloride post-digestion can improve sequence coverage in antibody peptide mapping compared with trypsin digestion.

A critical step in any troubleshooting exercise—but one that I think is underappreciated—is recognizing that there is a problem to be solved.

Are generic workflows really needed in environmental analysis? In the end, it is the analyst— not instrumentation or software—that is in charge of what data are obtained, after thoughtfully considering what sample preparation and acquisition method to use.

This method greatly facilitates the analysis of a large number of pesticides.

We continue our “Critical Evaluation” series with a consideration of liquid chromatography (LC) methods that use mass spectrometric detection.

Better sample preparation and miniaturized separations are enhancing these analyses.

Sample preparation and analysis of pharmaceuticals in wastewater present unique challenges. Here, we describe those challenges.

With a new prototype, we explore how to improve the resolution power and usability of LC–MS instruments for routine analysis in pharmaceutical applications.

Although well known, the extent of adduct ion formation in LC–ESI-MS/MS, and the implications for quantitative analysis and analyte characterization are not fully appreciated. We explore this problem and explain the implications for reproducibility, quantitative and qualitative analyses, and DDA and DIA.

With the advent of ambient ionization and portable mass spectrometers, the ability to perform rapid, on-site analysis is fast becoming a reality. We review the critical recent developments enabling this capability as well as remaining challenges that must be tackled to enable widespread adoption.

Advances in sample preparation have enabled analysis of RNA modifications in single cells, paving the way to discovering new insights into the functions of these molecules.

The benefits of tandem LC–MS are revealed.

Rudolf Krska and Michael Sulyok from the University of Natural Resources and Life Science in Vienna, Austria, discuss their recent work developing a multi‑analyte approach using liquid chromatography tandem mass spectrometry (LC–MS/MS), and the issues surrounding agrocontaminants in animal feeds.

Multidimensional liquid chromatography (MDLC) methods have revolutionized the characterization of complex drug modalities like antibodies–drug conjugates, antisense oligonucleotides, and small interfering RNA therapeutics.

Our annual review of new high performance liquid chromatography instruments, mass spectrometry systems, and data processing software.

Understanding the design and operation of a quadrupole mass analyzer can help you plan, optimize, and troubleshoot analytical methods.

Barry L. Karger and James P. Grinias are the winners of the 15th annual LCGC Lifetime Achievement and Emerging Leader in Chromatography Awards, respectively, for 2022. Here, we review their achievements.

The presence of per- and polyfluoroalkyl substances (PFAS) in products used every day by millions of people is a cause of concern among both consumers and scientists. PFAS found in drinking water and the environment can cause serious health issues in animals and humans. Amanda Belunis, who is a PhD candidate at the University of Maryland in Baltimore County, has been investigating the use of liquid chromatography­­–tandem mass spectrometry (LC–MS/MS) to detect PFAS from a variety of environmental sources. She spoke with us about methods used to detect PFAS and described a new method that she and her team have developed to enhance PFAS detection.

Reducing matrix effects during LC–MS/MS bioanalysis is paramount. Improving sample preparation techniques is the best way to combat this issue.

Method validation processes will need to be refined to better suit forensic laboratories.

Intact mass analysis is becoming increasingly useful for characterizing biologics. We describe the current application of intact mass analysis, including quantitation, sequencing, and structural characterization.

The UHPLC–MS/MS method can accurately determine the presence of these illegal feed additives in swine tissues.

Ensuring complete overlapping (coelution) of analyte and internal standard peaks can be critical for eliminating matrix effects in LC–MS/MS analysis.

The benefits of a robustness assessment for the analysis of a NIST mAb using a wide pore C4 LC column are described.

Quantification of Tau proteoforms in cerebrospinal fluid (CSF) could be useful to diagnose neurodegenerative diseases. LC–MS/MS results can help explain the underlying molecular mechanisms of disease evolution.