LC–MS

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This month we interview David Steiner, head of product development at LVA GmbH–Lebensmittelversuchsanstalt in Klosterneuburg, Austria about his work developing liquid chromatography–tandem mass spectrometry (LC–MS/MS) multiclass methods for classification of feedstuff, including replication of model complex feedstuffs in the analytical laboratory.

Electronic cigarettes and bottles with vape liquid on black background | Image Credit: © makcoud - stock.adobe.com

A study has used gas chromatography coupled to a time-of-flight mass spectrometer to build an electron ionization mass spectra database of more than 250 chemicals classified as either volatile or semi-volatile compounds. An additional, confirmatory layer of liquid chromatography–mass spectrometry analysis was subsequently performed.

Kevin Schug, the Shimadzu Distinguished Professor of Analytical Chemistry at The University of Texas at Arlington (UTA). Photo Credit: © Kevin Schug

In this edition of “Inside the Laboratory,” Kevin Schug, PhD, a full professor of chemistry at the University of Texas at Arlington, discusses his laboratory’s group work in environmental monitoring around water and soil quality near oil and gas extraction, using techniques such as liquid chromatography (LC), gas chromatography (GC), supercritical fluid chromatography (SFC), and coupling these techniques with mass spectrometry (MS).

cigarettes and tobacco | Image Credit: © Olexandr - stock.adobe.com.

This article describes a liquid chromatography–tandem mass spectrometry (LC–MS/MS) method for the analysis of coumarin in various tobacco matrices and electronic cigarette (E-cig) liquids, and highlights the importance of evaluating different MS/MS transitions of an analyte in complex sample matrices to overcome matrix effects. Matrix interfering components were separated from analyte using a C18 ultrahigh-pressure liquid chromatography (UHPLC) column with a larger inner diameter (3.0 mm, or 4.6 mm). Matrix suppressions on analyte responses were corrected by isotope dilution. Four different MS/MS transitions of coumarin were studied in each sample matrix to select a suitable MS/MS transition for analyte quantification based on matrix effects on each MS/MS transition. The method was validated using different tobacco matrices and E-cig liquids.

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