Application Notes: General

Size exclusion chromatography, or SEC, is a technique for separating proteins and other complex biomolecules in their native forms by size using aqueous eluents. For molecules of specific molecular weight, SEC can use used to detect and quantitate aggregates, fragments, monomers and dimer. This guide provides a comprehensive application overview for Biozen dSEC-2 Size Exclusion Columns.

An understanding of analyte hydrodynamic radius (Rh), as well as size exclusion column pore size and distribution, are all critical in the understanding of how an analyte will behave by SEC. In this white paper, we will explore several protein standards with known hydrodynamic radii, and how this can be used to determine ideal size exclusion conditions for optimal method performance.

Characterising various complex petrochemicals – including diesel, crude oil and vacuum gas oil – using the enhanced peak capacity of GC×GC–FID with thermal modulation

The described method of continuous flow rate monitoring provides real-time validation of total HPLC system performance and as such can improve reliability of results.

Aroma analysis is vital in plant-based meat production. Our study features the benefits of dynamic headspace extraction from cooked beef and plant-based meat.

This application highlights the ability of a compact GC to achieve results within six minutes utilizing a thick film column and hydrogen carrier gas.

This article describes how to achieve optimal separation of oligonucleotides and related impurities via a dedicated method development software package.

Featured Application: Semivolatiles on Rxi-SVOCms

Outlines an extraction procedure and an optional fluorenylmethyloxycarbonyl chloride derivatization step to allow for the analysis of glyphosate on a C18 column.

In this study, we present an ion-pairing-free method for AXPs analysis using microchip CE-MS. This fast, simple method achieves baseline resolution for Adenosine, AMP, ADP, and ATP. Excellent linearity and sensitivity are observed in human plasma.

identification and quantification of 34 target PFAS at parts-per-trillion levels in pork, plus retrospective analysis of sample data files for other untargeted PFAS.

The method conversion guidebook is an introduction to HALO® Fused-Core® technology, its benefits and how to take advantage of SPP for method conversion. This book will help you to facilitate converting conventional reversed-phase FPP separations to ultra-fast separations.

Built upon proven Fused-Core® technology for speed and efficiency, the HALO® PCS C18 is a positively charged surface chemistry designed to deliver improved peak shapes for basic compounds.

Following new USP guidelines for gradient method modernization enables both FPP and SPP methods to be improved for speed and solvent savings using HALO® Fused-Core® column technology while avoiding revalidation.

HPLC chromatographic methods can be greatly improved and modernized by simply reducing the column’s length and particle size. This not only saves time, but increases consumption and waste generation. By using modern HALO®Fused-Core® column technology, methods can be shortened thereby increasing laboratory efficiency while keeping back pressures under instrument pressure limitations.

HPLC and LC-MS separation materials and methodologies have evolved over the years to meet the challenges of the growing complexity of the separations themselves. In this Q&A, Stephanie Rosenberg, Director of Sales and Marketing discusses how utilizing the new HALO® positively charged surface columns impact the perforance of LC and LCMS separations for basic compounds.

This guide will discuss improved reversed phase biomolecule separation solutions for monoclonal antibodies, as well as chromatography columns used to develop an understanding of the protein backbone via peptide mapping. Glycosylation analysis using HILIC will also be discussed. Examples will highlight areas in the workflow where HALO® Fused-Core® columns with appropriate pore sizes and phases offer unique advantages.

A method is presented for the analysis of the chain-length distribution of inulin-type fructans with degree of polymerization up to 65 using SweetSep™ AEX200 column.

Separation and quantification of the 15 most commonly occurring carbohydrates in honey within 25 min is presented using Antec Scientific ALEXYS™ Carbohydrate Analyzer.

This application note outlines a QuEChERS method for the simultaneous analysis of cannabis for 67 pesticide and 5 mycotoxin residues in cannabis flower.

Antec Scientific developed a new column for the superior separation of all classes of carbohydrates using HPAEC with PAD or MS detection.

Antec Scientific ALEXYS Carbohydrate Analyzer is the most dedicated HPAEC-PAD instrument for the analysis of all classes of carbohydrates.

This application note explores the benefits of using LNI lab benches to enhance the performance of LC–MS systems.

Fast and sensitive analysis of lactose and isomers in ‘lactose-free’ labelled products using Antec Scientific SweetSep™ AEX200 HPAEC column.