Application Notes: General

Cannabinoid separation can be difficult with the presence of coeluting isomers. This application shows how a combination of LabSolutions MD (Method Development) software and powerful i-PDeA technology can help determine the optimal method for cannabinoid separation and deconvolute remaining co-eluting peaks after optimization.

This application showcases how a compact, affordable single quad LCMS can be easily integrated into existing laboratories for measurement of PFAS in diverse samples where adherence to regulated methods (e.g., EPA 533, 537.1, and 1633) is not required, or samples where expected concentrations are larger than sub ppb levels.

This application news presents instrument operating conditions and instrument method performance statistics, including method linearity, accuracy, and precision, for priority pollutant pesticides and PCB congeners. The method validated in this application acted as guidance in developing ASTM D8543 Test Method for Determination of OCPs and PCBs in Aqueous Solution by a GC/MS/MS which is intended for proposal to EPA as an alternative test procedure (ATP) for EPA 608.3

PFAS concerns demand sensitive testing. EPA Method 1621 screens for adsorbable organic fluorine (AOF). The Profiler-F Analyzer boasts sensitivity, recovery & precision.

This application note describes the evaluated quantification performance of eight nitrosamine impurities using an Agilent 6495D triple quadrupole LC/MS (6495D LC/TQ) system coupled with an Agilent 1290 Infinity II bio LC system and Agilent APCI source.

Recently, attention has been drawn to the use of microdroplet reactions for antibody analysis. The NIST IgG1 mAb was used to optimize microdroplet reaction conditions on two reactions, IdeS cleavage and disulfide bond reduction, using the Agilent Jet Stream ESI source.

In this app note you will learn how to develop a highly specific, sensitive, and reproducible method for the quantitation of N-nitroso dabigatran etexilate impurity in dabigatran etexilate mesylate drug substance using an Agilent 6495C triple quadrupole LC/MS (LC/TQ) coupled to an Agilent 1290 Infinity II LC.

This application note presents an alternative method using time-of-flight liquid chromatography/mass spectrometry (TOF LC/MS) and hydrophilic interaction chromatography (HILIC) that enables accurate and repeatable analysis of diverse oligonucleotides without using ion-pairing reagents.

Earlier assessment of molecules provides valuable insights into the manufacturing developability of molecules, reducing the need for unnecessary experimentation. The Intabio ZT system has the potential to make in-depth characterization of biopharmaceutical product charge variants quicker and more efficient to meet demanding timelines during early-phase clinical development.

In this podcast, we will look at different sources of potential heavy metal exposure and their concentrations in a variety of food products including fish, and beverages. In addition to the original research presented, we will compare other historical studies and public health findings to showcase the sources and concentrations of various heavy metals found in the food and water supply in order to build a more complete picture of heavy metal exposure through food and beverages.

This study examined the elemental composition of several types of chocolate products from chocolate liquor to milk and dark chocolate bars.

In this study, dried fruit samples (organic and regular raisins, sultanas and currants) and bread spreads (from different nuts and seeds; i.e. peanuts, almonds, sunflower etc.) were purchased from stores in the US, UK and Germany. Samples were digested using microwave digestion and tested by ICP-MS to determine heavy metal contamination and potential exposure.

Spices and hot sauces were purchased at dollar stores, farmer’s markets, chain stores, and online. Samples included ‘organic’ products. Cryogenic grinding and microwave digestion were employed in sample processing prior to ICP-MS analysis to determine the presence and level of heavy metal contamination and adulteration.

This new updated study revisits pet food brands first analyzed in 2009 and looks at new brands which that emerged after the 2007 pet food crisis and later enactment of FSMA to see if heavy metal contamination has significantly changed in the decade since the first study.

Professor Wan-Tang (Grace) Chen of the University of Massachusetts Lowell accepted our invite to participate in a virtual Q&A session. Dr. Chen directs the Plastics & Environment Research Laboratory at the university and was gracious enough to share what her team is working on, and how they’re leveraging the Pyrolysis-GC/MS technique in novel microplastics research.

This application note demonstrates the applicability of the Biozen dSEC-2 column in understanding the biophysical integrity monoclonal antibodies and related proteins. The hydrophilic nature of the Biozen dSEC-2 stationary phase shows favorable aggregate profiles for forced degradation work under high salt conditions which is a known challenge when working with more hydrophobic hybrid particles for these studies.

Size exclusion chromatography, or SEC, is a technique for separating proteins and other complex biomolecules in their native forms by size using aqueous eluents. For molecules of specific molecular weight, SEC can use used to detect and quantitate aggregates, fragments, monomers and dimer. This guide provides a comprehensive application overview for Biozen dSEC-2 Size Exclusion Columns.

An understanding of analyte hydrodynamic radius (Rh), as well as size exclusion column pore size and distribution, are all critical in the understanding of how an analyte will behave by SEC. In this white paper, we will explore several protein standards with known hydrodynamic radii, and how this can be used to determine ideal size exclusion conditions for optimal method performance.

Characterising various complex petrochemicals – including diesel, crude oil and vacuum gas oil – using the enhanced peak capacity of GC×GC–FID with thermal modulation

The described method of continuous flow rate monitoring provides real-time validation of total HPLC system performance and as such can improve reliability of results.

Aroma analysis is vital in plant-based meat production. Our study features the benefits of dynamic headspace extraction from cooked beef and plant-based meat.

This application highlights the ability of a compact GC to achieve results within six minutes utilizing a thick film column and hydrogen carrier gas.

This article describes how to achieve optimal separation of oligonucleotides and related impurities via a dedicated method development software package.

Featured Application: Semivolatiles on Rxi-SVOCms

ZipChip-MS streamlines mAb charge variant characterization, eliminating the need for method development. Data is seamlessly processed in Byos. This note outlines an expeditious workflow for biosimilarity assessment among mAbs.

Outlines an extraction procedure and an optional fluorenylmethyloxycarbonyl chloride derivatization step to allow for the analysis of glyphosate on a C18 column.