Application Notes: General

Merck streamlines chromatographic method development using in silico modeling to improve efficiency, reduce experimentation, and accelerate decision-making.

Potency testing workflows and key factors affecting quantitative accuracy, with a case study led guide to producing reliable, defensible data.

Artificial Intelligence or Artificial Stupidity? The Promises and Perils for Analytical Scientists

Artificial Intelligence or Artificial Stupidity? The Promises and Perils for Analytical Scientists

Track water quality in one IC run: measure key anions and disinfection byproducts (DBPs) with low-ppb sensitivity and strong resolution.

This executive summary explores how revisions to USP <621> have introduced greater flexibility in chromatographic method adjustments while reinforcing system suitability as the definitive standard for method acceptance. Heiko Behr, senior business development manager at Phenomenex, walks through key principles governing column substitution, flow rate scaling, mobile phase control, and dwell volume management during method transfer. A practical Nifedipine case study demonstrates how these allowances can be applied to reduce run times tenfold without compromising monograph-defined selectivity or compliance.

This issue of Current Trends in Mass Spectrometry brings together five perspectives on where mass spectrometry is heading next — from smarter polymer characterization and high-throughput food flavour analysis, to tackling food fraud, understanding ionic liquids, and expanding ion mobility techniques beyond proteomics. Whether you work in pharma, food science, environmental monitoring, or materials research, there's something here to inform and inspire.

Per- and polyfluoroalkyl substances (PFAS) are a diverse group of over 4000 organic fluorinated compounds, which have been widely used in industry since the 1950s. Due to their widespread presence and potential health risks, accurate PFAS analysis is essential to monitor contamination, ensure regulatory compliance, and protect human health and the environment. In this eBook, we explore our range of chromatography and mass spectrometry (MS) solutions for detecting very low levels of PFAS in matrices such as drinking water, wastewater, ambient air, foodstuffs, and consumer products. Plus, learn through a series of case studies how our LC-MS/MS systems and other analytical instruments (GCMS, CIC, QTOF…) meet and often exceed performance criteria included in EPA and AOAC methods for PFAS analysis – generating accurate results with fast turnaround times that enhance lab productivity.

Advances in analytical methods continue to enhance food authenticity testing, emphasizing untargeted fingerprinting, high-resolution mass spectrometry, and rapid portable tools. Techniques like DART-HRMS and LC–MS enable detection of adulteration at low levels, with robust validation and harmonization critical for legal and industrial confidence. Integrating chemometrics and developing standardized databases are key to addressing evolving food fraud challenges.

Achieve robust tirzepatide analysis through a structured RP method enabling consistent workflows and easy transfer to other GLP-1 receptor agonists.

Ensure confident PFOS detection in complex food matrices with advanced LC-MS separation using the bioinert YMC Accura Triart C18 column.

This article explains why carrier gas selection remains critical for modern gas chromatography (GC) and GC–MS workflows. The discussion explores the ongoing relevance of helium, the performance advantages of BIP® Helium for high-sensitivity applications, and how laboratories can balance cost, efficiency, and sustainability through informed carrier gas strategies.

Accelerate a novel approach for mRNA characterization with innovative 2D-LC fingerprinting using a bioinert-coated YMC Accura Bio Pro IEX QF column.

Achieve precise purity analysis of peptide-oligonucleotide conjugates using an optimized IP-RP method with a bioinert YMC Accura Triart Bio C18 column for reliable QC.

Simplify mRNA encapsulation efficiency analysis with robust AEX separation delivering precise results and deeper insight into LNP quality.

BrightSpec's headspace-MRR platform eliminates the need for chemical derivatization in formaldehyde quantitation achieving reproducible detection across a wide dynamic range

BrightSpec's platform achieved LODs of 3 ppm for EG and 60 ppm for DEG in PEG-400. Linearity was strong and repeatability remained under 4%.

Accelerate oligonucleotide analysis with bioinert YMC Accura BioPro IEX QF column enabling high-resolution separation in under two minutes for efficient QC workflows.

Structural misassignment risks propagating errors. MRR spectroscopy with molecular modeling discriminates viridiflorol, globulol, and stereoisomers sans reference standards.

Improved analysis of tryptophan and its metabolites using a bioinert YMC Accura Triart C18 column enabling reliable results in complex biological matrices.

BrightSpec's isoMRR™ platform directly detects EtO and acetaldehyde in PEG-3350, meeting USP LOQ criteria in under one minute with no sample prep

This application note demonstrates how BrightSpec's platform provides a faster, simpler alternative to GC-MS for residual solvent analysis in pharmaceutical products

Fast GC/MS method analyzes 76 pesticides per EPA 525 in under 8.5 min using the PerkinElmer GC system, ensuring compliance, sensitivity, and resolution.

Fast GC/MS method for SVOC per EPA 8270E: 87 compounds analyzed in ~14 min with high accuracy, compliance, and repeatability

As regulatory mandates push PFAS detection limits toward single-digit PPT levels, the pervasive threat of background contamination from solvents and instrument components has emerged as a defining challenge for modern laboratories. Proper data defensibility requires a rigorous, end-to-end mitigation strategy that prioritizes the replacement of fluoropolymer materials with inert alternatives as well as the implementation of delay columns to isolate system-derived interference. Ultimately, adopting a proactive approach to workflow integrity, through the use of certified consumables and specialized tools, is essential for laboratories to stay ahead of tightening environmental standards and expanding target lists.

Explore practical strategies for reducing PFAS contamination risks in food testing workflows with guidance on troubleshooting, blank management, chromatography optimization, and contamination control. This quick-reference resource highlights common sources of false positives and false negatives while outlining best practices for achieving reliable low-ppt PFAS analysis across complex food matrices.

Stay current on the evolving global PFAS regulatory landscape with insights into food testing legislation, compliance requirements, and analytical methods across Europe, the United States, and China. Explore guidance on PFAS limits in food and packaging, LC/MS/MS testing workflows, and strategies for accurate, high-sensitivity analysis in complex food matrices. Learn how laboratories can streamline PFAS testing while supporting regulatory readiness and food safety confidence.

Struggling with PFAS analysis in complex food and beverage samples? In this application note, Agilent demonstrates a streamlined workflow for the determination of PFAS in beer and wine using QuEChERS extraction followed by Captiva EMR PFAS Food I cleanup