Application Notes: General

Rapid HS-GC/FID method quantifies ethanol in food and drink with high sensitivity, robust calibration, and compliance across global standards.

GC-FID enables accurate benzene analysis in USP Carbomer 940, meeting system suitability and supporting compliant pharmaceutical quality control workflows.

Fast GC/MS method for SVOC per EPA 8270E: 87 compounds analyzed in ~14 min with high accuracy, compliance, and repeatability

Agilent LC/MS systems and software are designed for robustness, ease of use, and consistent performance, helping labs ensure higher productivity and lower operating costs. Integrating seamlessly with Agilent Infinity III LC systems and Agilent OpenLab CDS software, the Agilent InfinityLab Pro iQ Series redefines LC/MS accessibility by combining advanced performance with intelligent design. Read the new white paper to discover how to maximize your ROI with the Agilent InfinityLab Pro iQ Series.

The Central Dogma of Molecular Biology states the genetic information stored in our DNA is transcribed into an intermediate molecule, RNA, which is then translated into protein. There are three main types of RNA associated with transcription/translation: messenger RNA, ribosomal RNA, and transfer RNA. All RNAs in the biological system carry post transcriptional modifications: chemical functional groups added to the base, the ribose sugar, or both. The gold standard in analytical technology for identification and characterization of these modifications remains steadfast in the realm of liquid chromatography hyphenated to high resolution accurate mass spectrometry. This paper covers how the analysis of native RNA is technically demanding and how RP-based separation strategies that avoid ion-pairing reagents are in demand. It highlights the use of Orbitrap Tribid MS for system evaluation with and without sulfolane to assess retention, signal stability, and fragmentation efficiency.

The last AppNote in this Newsletter shows how liquid-liquid extraction of xylazine and medetomidine in plasma and urine can be fully automated and seamlessly combined with LC-MS/MS analysis. All critical steps - including extraction, mixing, centrifugation, evaporation, and solvent exchange - are performed by the MPS, enabling high-throughput workflows with excellent accuracy and precision at low concentration levels.

This AppNote highlights how salting-out assisted liquid-liquid extraction (SALLE) can be fully automated for the determination of bisphenol A (BPA) in beverages. The study demonstrates how automation removes sample-prep bottlenecks while ensuring consistent, robust results across a wide range of beverage matrices.

This AppNote demonstrates how liquid-liquid extraction for fatty acid methyl esters (FAMEs) in infant formula can be reliably automated in accordance with AOAC® 2012.13. Using the GERSTEL MPS, critical timing, mixing, and reagent addition steps are precisely controlled, ensuring reproducible and robust results.

This AppNote shows how manual liquid-liquid extraction can be directly translated into a fully automated workflow using the GERSTEL MPS. The result: highly reproducible data, reduced solvent exposure, and far less hands-on time for laboratory staff. Even complex steps such as mixing, evaporation, and reconstitution are handled reliably - making LLE more efficient and better suited for routine analysis.

Innovation and quality drive every stage of beverage production—from early R&D to the final sip. Advanced GCMS and Molecular Spectroscopy solutions help producers ensure excellence across the entire beverage life cycle, from R&D and regulation/safety compliance to QA/QC, shelf life, and packaging applications. Whether crafting coffee, soft drinks, or alcoholic beverages, this infographic describes how Shimadzu’s technology elevates the beverage-making process to ensure reliable, consistent, quality products.

Discover our comprehensive portfolio of analytical HPLC solutions, featuring the latest Agilent Infinity III LC Series, applications, and a selection guide.

How lab managers and analytical scientists can maximize ROI, minimize hidden costs, and drive sustainable profitability with smarter LC decisions

Overcome common HPLC challenges. This eBook offers solutions to simplify routines, ensure reliable results, make smart investments, and create a sustainable lab.

How lab managers and analytical scientists can maximize ROI, minimize hidden costs, and drive sustainable profitability with smarter LC decisions

Streamline your workflow with smart automation

The quality and consistency of lipid nanoparticles (LNPs) depend on the purity of their component lipids—a challenge that demands robust purification strategies. Using high-purity silica chromatography resins help enable consistent, scalable purification of those component lipids. Read the article to uncover three major challenges in the purification process, and the variety of ways high quality silica resins can help overcome the challenges of chromatographic process development and scale-up for lipid purification.

Not all chromatography resins are created equal. Off-the shelf chromatography resins might not always meet the rigorous purification requirements of biopharmaceutical manufacturing. Custom bonded silica can address a wide range of separation challenges, leading to real performance improvements. Discover more about the latest innovations in chromatography silica.

Enzymes in their free form can present challenges, but immobilizing enzymes on solid silica supports can significantly improve enzyme stability, recovery, and reusability. Silica’s mechanical strength and chemical robustness enable consistent performance, easier purification, and cost‑effective reuse. Read the article to learn more about the advantages of solid silica supports for enzyme immobilization.

Particularly in the pharmaceutical industry, drug purity isn’t just a goal – it’s essential for achieving safety, stability and efficacy. However, purification is easier said than done, especially with challenging molecules like DNA and RNA “oligonucleotides,” due in large part to their diversity and the range of impurities that can be generated during production. Before you choose a chromatography resin for your next purification application, consider these five characteristics. Download the article to learn more.

Hamilton's PRP-Z2 stationary phase enables fast, robust separation of diquat and paraquat herbicides under EPA 549.2 and EPA 539 conditions.