Application Notes: General

This application note highlights a new multi-modal LC-MS/MS approach enabling robust analysis of polar and ionic metabolites without derivatization or ion-pairing.

Highlights amino acid analysis of rice and millets using a high-speed amino acid analyzer with a protein hydrolysate method, supporting nutritional characterization.

Capillary gel electrophoresis (CGE) is a high-resolution, label-free technique for the quality control of mRNA-based therapies. Because the poly(A) tail plays a critical role in mRNA stability and translation efficiency, accurately detecting and quantifying tailless species is essential to ensuring therapeutic efficacy and patient safety. This article explores how to optimize the SCIEX BioPhase™ 8800 system and RNA 9000 Purity & Integrity Kit to achieve the resolution required for today’s advanced mRNA medicines. Key method parameters, including electric field strength, capillary temperature, and sample concentration, are examined to support reliable separation and impurity detection.

In this case study, an improvement in oligonucleotide peak shape is shown by suppressing the sample solvent effect through simultaneous injection with a desired solvent while using dedicated method development software to comprehensively evaluate co-injection solvent types and volumes to enable a more efficient workflow.

The article describes a comprehensive automated prep purification workflow targeting semaglutide, a GLP-1 receptor agonist, using dedicated method development software to automatically optimize a gradient profile and search for conditions that meet the specified separation criteria. This was followed by scale-up to purification scale and streamlined confirmation of the purities of collected fractions using benchtop MALDI-TOF MS.

This article describes how to efficiently achieve the optimal peak separation for charge variants of a monoclonal antibody using pH gradient ion-exchange chromatography and LabSolutions MD, dedicated software that can automate the entire method development workflow, including the generation of an analysis schedule, mobile phase preparation, and data processing.

This article describes the efficient development of robust analytical methods based on Analytical Quality by Design (AQbD) using dedicated LabSolutions MD software, which supports each phase of the process – screening, optimization, and validation – using functions such as experimental design, building of design space by automatic Peak Tracking, and robustness evaluation.

This article describes how dedicated software for supporting method development, in conjunction with single quad LCMS and inert UHPLC, can improve the overall method development workflow and efficiently achieve the optimal separation of oligonucleotides and related impurities.

This case study illustrates the streamlining of SFC method development using a mixture of six small molecule pharmaceuticals as a model sample, from automatic screening of columns and modifiers to design space evaluation for optimal conditions.

Identification of polyphenolic catechin compounds for health and industrial quantification and quality control of tea products.

Stay ahead with Picus® 2, the ergonomic electronic pipette designed for effortless, repeatable pipetting. Intuitive for all users yet packed with advanced features, Picus® 2 connects seamlessly to the Sartorius Pipetting mobile app, streamlining workflows, automating settings, and keeping your pipette updated. Smart, connected, and built for the future—because your lab deserves the best.

For sulfur compounds in the sub-ppmv to ppbv and lower concentrations, dynamically generated gas standards prevent the need for storing reactive and unstable components

This report shows how Hitachi’s Amino Acid Analyzer delivers high-speed, high-resolution analysis of HCl-hydrolyzed cat, dog, bird, and goldfish pet food samples

This eBook presents compelling examples of how the Agilent InfinityLab Pro iQ Plus mass detector can significantly elevate analysis and QC in biopharma.

The KNAUER Sepapure oliGO column is a good alternative to the commonly used competitor column for oligonucleotide analysis.

This technical note demonstrates a sensitive method for the quantitation of pasireotide in human plasma using high-resolution accurate mass spectrometry. A lower limit of quantitation (LLOQ) of 0.05 ng/mL was achieved in extracted plasma samples.

Explore best practices for LC-MS analysis of oligonucleotides to get the best results possible, quickly and easily.

This technical note demonstrates a highly sensitive, robust and rapid workflow to quantify TL 13-112, a selective ALK degrader, and its inactive control, TL 13-110, in rat plasma using a high-end triple quadrupole mass spectrometer.

This webinar covers quantitative bioanalysis strategies to support mRNA drug development using LC-MS, including sample preparation, surrogate peptide selection, LC-MS method development, and verification/validation.

Discover how Arium® Comfort I UV system supports precise PFAS detection, reducing contaminants to sub-ppt levels while improving sustainability and cost efficiency.

This technical note demonstrates a sensitive method to quantify a glucagon-like peptide-1 (GLP-1) analog, semaglutide, in rat plasma on a high-end triple quadrupole mass spectrometer.

Per- and Polyfluoroalkyl Substances (PFAS), a family of more than 4,700 chemical molecules, are known for their non-stick and waterproof properties. These versatile compounds find their application in a variety of industries, including cosmetics, textiles, and automobiles. PFAS exhibit remarkable resistance to degradation, which has led to their pervasive distribution in the environment and consequent human exposure. The effects of such exposure are far-reaching, including adverse effects on fertility, developmental abnormalities, cholesterol levels, immune function, and an increased risk of certain cancers. In addition to their toxicological effects, PFAS have become a global concern as regulators and researchers strive to understand the extent of their impact and develop effective methods for detection and mitigation. This collection of articles brings together research on the contamination and effects of PFAS in air, soil, and water, shedding light on the complex challenges posed by these persistent substances in our environment. Overall, this collection of articles seeks to advance researchers' knowledge of the dynamics, effects, and detection of PFAS.