Application Notes: General

In this application note the charge variant characterisation of IgG4-based mAbs was performed using a BioPro IEX QF anion exchange column coupled to an MS detector.

This application note provides you an optimum RP method for the DAR determination of a site-specific ADC using a widepore YMC-Triart Bio C4 column.

With this AEX method a mix of six oligonucleotides can be separated in under two minutes.

This Application Note presents an optimised IP-RP method for a model POC, providing a reliable foundation for quality control workflows.

This step-by-step method development study for a GLP-1 receptor agonist provides a robust and precise analytical workflow for purity testing of tirzepatide.

This application with data from Roche Diagnostics confirms superior performance of bioinert-coated YMC Accura BioPro IEX SF columns for the IEX-MS analysis of mAbs.

This non-IP-RP method, using ammonium bicarbonate as mobile phase additive, is highly sensitive and suitable for the bioanalysis of therapeutic oligonucleotides.

We understand you are busy, needing to prioritize running instruments, reporting results and managing your laboratory to meet deadlines. We created this solution guide to explain how SCIEX systems fit in the drug development pipeline to save you time evaluating options. This guide covers bio/pharmaceutical quantitative assays from discovery to QA/QC, for large and small molecules. Happy reading!

As a leader responsible for metabolite identification in your organization, what keeps you awake at night? SCIEX can give you peace of mind by helping you . . .

Learn how to prevent contamination in pipetting. This guide covers pipette-to-sample, sample-to-pipette, and sample-to-sample risks, with best practices like filter tips and sterile techniques.

Prevent contamination in pipetting with best practices to avoid pipette-to-sample, sample-to-pipette, and sample-to-sample risks. Learn how filter tips and sterile techniques safeguard results.

Overcome common challenges in 3D cell culture pipetting. Learn tips for handling viscous hydrogels, transferring spheroids, reducing contamination, and improving throughput with the right techniques.

Discover best practices for reproducible 3D cell culture workflows. Learn how Picus® 2 pipettes and GrowDex® hydrogels improve accuracy, reduce variability, and streamline sample prep.

This note presents platform methods to characterize ADC using BioCore columns: SEC for aggregate determination, HIC/RP for DAR assesment, CEX for charged variant analysis.

Explore how next-generation high-performance liquid chromatography (HPLC) systems are elevating biopharmaceutical analysis. This eBook showcases innovations in size-exclusion chromatography (SEC), method updates, and proven approaches to increase efficiency, consistency, and resolution in the lab.

Free enzymes suffer from inconsistent performance and are difficult to recover and reuse. Immobilizing enzymes on silica resin offers a practical and efficient solution for biocatalysis reactions, enabling reproducibility, consistency, and cost-effectiveness at scale. Grace’s DAVISIL® silica resins offer a smart solution for immobilizing enzymes and are available in a wide range of pore sizes, particle sizes and chromatography phases. Discover how solid silica supports from Grace can transform your process.

Measure perchlorate in water at trace levels with ion chromatography. This USEPA 314.0-compliant method delivers accurate, interference-free results to 1 ppb.

TFA in LC causes baseline noise due to UV absorbance, making pump pulsation visible and raising LOD/LOQ. Increasing mixer volume stabilizes baseline.

Shows how TD-GC-MS enables rapid in-house screening of chemical emissions from construction and consumer materials.

Outlines a method for detecting trace PFAS emissions from packaging and cookware using dynamic headspace with TD-GC-MS.

By using a streamlined native hydrophobic interaction chromatography-mass spectrometry (HIC-MS) method, MS-compatible ammonium tartrate salts can enhance the separation and detection of antibody-drug conjugate (ADC) drug-to-antibody ratio (DAR) species. This advanced approach overcomes historical coupling difficulties and supports rapid, high-resolution DAR profiling and real-time monitoring for improved ADC characterization and manufacturing control.

Presents a TD-GC-MS method for ppt-level detection of PAHs in air, offering a simpler alternative to solvent-based protocols.

Details how sorbent-based breath sampling combines with TD-GC-MS supports VOC biomarker discovery and route screening.

A practical overview of the technique introduces the principles, instrumentation, and typical sample of workflows used in TD-GC-MS.

Poster showcasing Pfizer's use of ACD/Labs’ method development software to automate method variation simulations. Highlights key tools and features that streamline method transfer and boost efficiency through automation.

This poster presents an example of the analysis of all 17 explosives listed in EPA 8330B using atmospheric pressure chemical ionization liquid chromatography mass spectrometry (APCI-LC-MS/MS), which allowed for more sensitive and selective analysis than PDA detection. In addition, MRM analysis provides an extra layer of confirmation based on the unique fragmentation of each compound.