Application Notes: General

GPC method development, optimization, and data interpretation can be complex and time-consuming. To support you in overcoming these challenges, we have compiled a collection of application notes covering the GPC analysis of a wide range of polymers. This application notebook provides practical insights, real-world examples, and proven methods to help you improve accuracy, streamline your workflow, and get the most from your GPC data.

When coupled with gel permeation/size exclusion chromatography, light scattering detection allows the measurement of true – or “absolute” – molecular weight (MW) of macromolecules, as opposed to the relative values obtained from conventional column calibration methods. The LenS3 MALS detector is a static multi-angle light scattering detector compatible with any UHPLC, HPLC, SEC, or GPC systems, designed to determine molecular weight and size within the range of 200-108 g/mol and 2-300 nm. This guide describes the technology, features, and functions of the LenS3 MALS Detector, along with application data examples. Learn how easy it is to utilize MALS in your polymer characterization.

Static light scattering detectors can be coupled to SEC/GPC systems for the determination of true molecular weight and size of polymers or biomolecules-independent of column calibration. This book is an essential resource for scientists and analysts using SEC or GPC, offering clear explanations of light scattering fundamentals, traditional methodologies, and the latest advancements in the field. Whether you're looking to deepen your understanding or improve your analytical accuracy, this guide offers both theory and practical insights.

As pharmaceutical laboratories face increasing pressure to improve efficiency, sustainability, and analytical precision, the demand for highly repeatable, resource-efficient instrumentation is on the rise. Capillary-scale liquid chromatography (LC) is reshaping expectations, enabling labs to achieve high-impact results without added overhead.

Identification of polyphenolic catechin compounds for health and industrial quantification and quality control of tea products.

A novel HIC column featuring innovative particle technology is benchmarked for separation of high DAR species ADCs, against a reference column with conventional chemistry.

PROTACs are protein degraders that harness the cell’s disposal machinery to remove specific targets. Now advancing into clinical trials, they show high potency at nanomolar levels. To support safe and effective drug development, sensitive and selective assays are essential for reliable detection and quantification.

Join us to see how SCIEX OS software helps you meet regulatory requirements with confidence. This webinar highlights new features and tools that strengthen data integrity, improve quality control, and save valuable lab time.

This infographic explores the benefits of Acoustic Ejection Mass Spectrometry (AEMS)- based workflows for efficient small and large molecule screening.

In this issue of “Hot Topics in Mass Spectrometry,” we highlight the role of ion mobility mass spectrometry (IM-MS) in addressing challenges in omics research, such as resolving isomeric ambiguity and enhancing sensitivity for low-abundance analytes, how selected ion flow tube mass spectrometry (SIFT-MS) is being used to conduct objective odor analysis in food and environmental samples, and the importance of sample preparation in quantitative bioanalysis.

We evaluated the accuracy of lipid quantitation in human plasma by LC-MS using single-point or multipoint calibration curves with authentic or surrogate standards.

This selection poster will guide you to find the best preparative LC column for your analysis with tips and guidance for stationary phase, the purification workflow, instrument compatibility, and links to useful resources such as an LC Scaling Calculator.

Hot Topics in PFAS” from LCGC International, we provide a comprehensive overview of per- and polyfluoroalkyl substances (PFAS) analysis, highlighting the evolving analytical techniques, challenges, and regulatory landscape surrounding these persistent chemicals. Key analytical challenges, such as matrix effects, sample contamination, and the lack of commercial standards, are addressed, alongside innovations like matrix-matched semiquantification for complex samples and the use of specialized HPLC columns to retain ultra-short-chain PFAS.

Efficiently desalt diverse peptides with Tecan Narrow Bore Extraction™ (NBE) columns. Reduce drying time, automate SPE cleanup, and prepare high quality samples for LC MS/MS.

Streamline proteomics with Tecan’s NBE format for rapid on-column digestion, minimizing sample loss and boosting analyte recovery.

This SEC-MS method demonstrates the precise separation of a single-chain variable fragment from its drug-conjugate.

In this application note the charge variant characterisation of IgG4-based mAbs was performed using a BioPro IEX QF anion exchange column coupled to an MS detector.

This application note provides you an optimum RP method for the DAR determination of a site-specific ADC using a widepore YMC-Triart Bio C4 column.

With this AEX method a mix of six oligonucleotides can be separated in under two minutes.

This Application Note presents an optimised IP-RP method for a model POC, providing a reliable foundation for quality control workflows.

This step-by-step method development study for a GLP-1 receptor agonist provides a robust and precise analytical workflow for purity testing of tirzepatide.

This application with data from Roche Diagnostics confirms superior performance of bioinert-coated YMC Accura BioPro IEX SF columns for the IEX-MS analysis of mAbs.

This non-IP-RP method, using ammonium bicarbonate as mobile phase additive, is highly sensitive and suitable for the bioanalysis of therapeutic oligonucleotides.

We understand you are busy, needing to prioritize running instruments, reporting results and managing your laboratory to meet deadlines. We created this solution guide to explain how SCIEX systems fit in the drug development pipeline to save you time evaluating options. This guide covers bio/pharmaceutical quantitative assays from discovery to QA/QC, for large and small molecules. Happy reading!

As a leader responsible for metabolite identification in your organization, what keeps you awake at night? SCIEX can give you peace of mind by helping you . . .

Corteva is driving sustainable agriculture by streamlining HPLC method development to save time, scale efficiently, and help meet global food demands despite climate and resource challenges.

Learn how to prevent contamination in pipetting. This guide covers pipette-to-sample, sample-to-pipette, and sample-to-sample risks, with best practices like filter tips and sterile techniques.