Application Notes: General

Overcome common challenges in 3D cell culture pipetting. Learn tips for handling viscous hydrogels, transferring spheroids, reducing contamination, and improving throughput with the right techniques.

Discover best practices for reproducible 3D cell culture workflows. Learn how Picus® 2 pipettes and GrowDex® hydrogels improve accuracy, reduce variability, and streamline sample prep.

This note presents platform methods to characterize ADC using BioCore columns: SEC for aggregate determination, HIC/RP for DAR assesment, CEX for charged variant analysis.

Explore how next-generation high-performance liquid chromatography (HPLC) systems are elevating biopharmaceutical analysis. This eBook showcases innovations in size-exclusion chromatography (SEC), method updates, and proven approaches to increase efficiency, consistency, and resolution in the lab.

Free enzymes suffer from inconsistent performance and are difficult to recover and reuse. Immobilizing enzymes on silica resin offers a practical and efficient solution for biocatalysis reactions, enabling reproducibility, consistency, and cost-effectiveness at scale. Grace’s DAVISIL® silica resins offer a smart solution for immobilizing enzymes and are available in a wide range of pore sizes, particle sizes and chromatography phases. Discover how solid silica supports from Grace can transform your process.

Measure perchlorate in water at trace levels with ion chromatography. This USEPA 314.0-compliant method delivers accurate, interference-free results to 1 ppb.

TFA in LC causes baseline noise due to UV absorbance, making pump pulsation visible and raising LOD/LOQ. Increasing mixer volume stabilizes baseline.

Shows how TD-GC-MS enables rapid in-house screening of chemical emissions from construction and consumer materials.

By using a streamlined native hydrophobic interaction chromatography-mass spectrometry (HIC-MS) method, MS-compatible ammonium tartrate salts can enhance the separation and detection of antibody-drug conjugate (ADC) drug-to-antibody ratio (DAR) species. This advanced approach overcomes historical coupling difficulties and supports rapid, high-resolution DAR profiling and real-time monitoring for improved ADC characterization and manufacturing control.

Presents a TD-GC-MS method for ppt-level detection of PAHs in air, offering a simpler alternative to solvent-based protocols.

Details how sorbent-based breath sampling combines with TD-GC-MS supports VOC biomarker discovery and route screening.

A practical overview of the technique introduces the principles, instrumentation, and typical sample of workflows used in TD-GC-MS.

Poster showcasing Pfizer's use of ACD/Labs’ method development software to automate method variation simulations. Highlights key tools and features that streamline method transfer and boost efficiency through automation.

This poster presents an example of the analysis of all 17 explosives listed in EPA 8330B using atmospheric pressure chemical ionization liquid chromatography mass spectrometry (APCI-LC-MS/MS), which allowed for more sensitive and selective analysis than PDA detection. In addition, MRM analysis provides an extra layer of confirmation based on the unique fragmentation of each compound.

This poster presents a comprehensive, multi-approach data processing method for the analysis of non-targeted PFAS in both positive and negative ionization modes. Results show this three-pronged approach gave comprehensive identifications without excess false positives.

This poster shows the development and validation of a fully automated sample preparation and subsequent LC-MS/MS with trap-and-elute chromatography assay analyzing 25-hydroxyvitamin-D2 and D3. With this automated platform, technicians can significantly reduce their active time in the sample preparation workflow, focusing primarily on sample registration and results review.

EPA Method 1633A is currently the only validated multi-lab method for PFAS detection in leachate. This poster demonstrates the effectiveness of a system to provide robust, stable performance over 154 injections and 37 hours of continuous PFAS analysis, making it ideal for routine monitoring of complex leachate matrices.

With this AEX method a mix of six oligonucleotides can be separated in under two minutes.

New automated workflow enables fast comparison of chromatograms, revealing sample differences and simplifying essential oil quality control.

Smart Subtract simplifies GC×GC–MS data analysis, helping identify trace odours in recycled plastics faster and more accurately.

Cryogen-free TD–GC–MS method enables 10 ppt detection of ethylene oxide from fugitive sources, compliant with draft US EPA Method 327.

Cryogen-free TD–GC–MS method enables ppt-level analysis of 30 volatile fluorinated compounds released during PFAS destruction, compliant with US EPA OTM-50.

High-capacity sorptive extraction with GC–MS enables trace analysis of odorants and chlorophenols in water, combining ppt sensitivity with full automation.

Trap-enhanced SPME Arrow analysis improves aroma profiling in food, enabling better peak shape, greater analyte coverage, and a wider concentration range.

John Yates’ lab is using MOBILion’s Parallel Accumulation Mobility Aligned Fragmentation (PAMAF) technology to enhance the DeGlyPHER workflow, which enables detailed, site-specific analysis of N-glycosylation in viral spike proteins such as SARS-CoV-2. This method combines sequential deglycosylation with high-sensitivity LC-MS to quantify glycan occupancy and processing, offering insights into the relationship between glycosylation and immune response. DeGlyPHER has been applied to over 200 vaccine candidates, including those for HIV, influenza, and Ebola, advancing understanding of glycoprotein-based immunogen design. This executive summary explains how DeGlyPHER is transforming vaccine development in real time, driving progress toward more effective and consistent immunization strategies, and delivering a more sensitive and high-throughput approach compared to traditional glycoproteomic methods.

Discover the supremacy of the bioinert coated hardware of YMC Accura BioPro IEX QF columns for reproducible analysis of oligonucleotides in ion-exchange chromatography.