Application Notes: General

A practical guide to LC–MS calibration, comparing external, surrogate and in sample approaches to control matrix effects and support accurate, regulator aligned bioanalysis.

GC-FID enables fast, reproducible analysis of 37 FAMEs in edible oils, improving fatty acid profiling for quality, authenticity, and adulteration detection.

Ensuring the safety of drinking water is a critical public health priority shaped by increasingly stringent global regulations. This eBook provides a focused guide to meeting the requirements of EU Directive 2020/2184, outlining advanced analytical strategies for detecting a wide range of contaminants, from persistent organic pollutants and disinfection by-products to trace-level metals. The directive sets ambitious monitoring and quality standards designed to protect public health across increasingly complex water systems.

HS-GC-FID enables compliant residual solvent analysis per USP 467, delivering accurate quantitation, full separation, and improved productivity in pharma labs.

A robust HS-GC-FID workflow on the PerkinElmer GC system enables accurate, reproducible USP 467 residual solvent analysis with streamlined, 21 CFR Part 11–compliant data control.

Fast GC/MS method analyzes 76 pesticides per EPA 525 in under 8.5 min using the PerkinElmer GC system, ensuring compliance, sensitivity, and resolution.

Rapid HS-GC/FID method quantifies ethanol in food and drink with high sensitivity, robust calibration, and compliance across global standards.

GC-FID enables accurate benzene analysis in USP Carbomer 940, meeting system suitability and supporting compliant pharmaceutical quality control workflows.

Agilent LC/MS systems and software are designed for robustness, ease of use, and consistent performance, helping labs ensure higher productivity and lower operating costs. Integrating seamlessly with Agilent Infinity III LC systems and Agilent OpenLab CDS software, the Agilent InfinityLab Pro iQ Series redefines LC/MS accessibility by combining advanced performance with intelligent design. Read the new white paper to discover how to maximize your ROI with the Agilent InfinityLab Pro iQ Series.

The Central Dogma of Molecular Biology states the genetic information stored in our DNA is transcribed into an intermediate molecule, RNA, which is then translated into protein. There are three main types of RNA associated with transcription/translation: messenger RNA, ribosomal RNA, and transfer RNA. All RNAs in the biological system carry post transcriptional modifications: chemical functional groups added to the base, the ribose sugar, or both. The gold standard in analytical technology for identification and characterization of these modifications remains steadfast in the realm of liquid chromatography hyphenated to high resolution accurate mass spectrometry. This paper covers how the analysis of native RNA is technically demanding and how RP-based separation strategies that avoid ion-pairing reagents are in demand. It highlights the use of Orbitrap Tribid MS for system evaluation with and without sulfolane to assess retention, signal stability, and fragmentation efficiency.

The last AppNote in this Newsletter shows how liquid-liquid extraction of xylazine and medetomidine in plasma and urine can be fully automated and seamlessly combined with LC-MS/MS analysis. All critical steps - including extraction, mixing, centrifugation, evaporation, and solvent exchange - are performed by the MPS, enabling high-throughput workflows with excellent accuracy and precision at low concentration levels.

This AppNote highlights how salting-out assisted liquid-liquid extraction (SALLE) can be fully automated for the determination of bisphenol A (BPA) in beverages. The study demonstrates how automation removes sample-prep bottlenecks while ensuring consistent, robust results across a wide range of beverage matrices.

This AppNote demonstrates how liquid-liquid extraction for fatty acid methyl esters (FAMEs) in infant formula can be reliably automated in accordance with AOAC® 2012.13. Using the GERSTEL MPS, critical timing, mixing, and reagent addition steps are precisely controlled, ensuring reproducible and robust results.

This AppNote shows how manual liquid-liquid extraction can be directly translated into a fully automated workflow using the GERSTEL MPS. The result: highly reproducible data, reduced solvent exposure, and far less hands-on time for laboratory staff. Even complex steps such as mixing, evaporation, and reconstitution are handled reliably - making LLE more efficient and better suited for routine analysis.

Fully automated determination of 3-MCPD, 2-MCPD and glycidol esters in edible oils using ISO 18363-4 with automated sample preparation and GC-MS/MS detection.

Innovation and quality drive every stage of beverage production—from early R&D to the final sip. Advanced GCMS and Molecular Spectroscopy solutions help producers ensure excellence across the entire beverage life cycle, from R&D and regulation/safety compliance to QA/QC, shelf life, and packaging applications. Whether crafting coffee, soft drinks, or alcoholic beverages, this infographic describes how Shimadzu’s technology elevates the beverage-making process to ensure reliable, consistent, quality products.

Discover our comprehensive portfolio of analytical HPLC solutions, featuring the latest Agilent Infinity III LC Series, applications, and a selection guide.

How lab managers and analytical scientists can maximize ROI, minimize hidden costs, and drive sustainable profitability with smarter LC decisions

Overcome common HPLC challenges. This eBook offers solutions to simplify routines, ensure reliable results, make smart investments, and create a sustainable lab.

How lab managers and analytical scientists can maximize ROI, minimize hidden costs, and drive sustainable profitability with smarter LC decisions

Streamline your workflow with smart automation