Application Notes: General

The quality and consistency of lipid nanoparticles (LNPs) depend on the purity of their component lipids—a challenge that demands robust purification strategies. Using high-purity silica chromatography resins help enable consistent, scalable purification of those component lipids. Read the article to uncover three major challenges in the purification process, and the variety of ways high quality silica resins can help overcome the challenges of chromatographic process development and scale-up for lipid purification.

Not all chromatography resins are created equal. Off-the shelf chromatography resins might not always meet the rigorous purification requirements of biopharmaceutical manufacturing. Custom bonded silica can address a wide range of separation challenges, leading to real performance improvements. Discover more about the latest innovations in chromatography silica.

Enzymes in their free form can present challenges, but immobilizing enzymes on solid silica supports can significantly improve enzyme stability, recovery, and reusability. Silica’s mechanical strength and chemical robustness enable consistent performance, easier purification, and cost‑effective reuse. Read the article to learn more about the advantages of solid silica supports for enzyme immobilization.

Particularly in the pharmaceutical industry, drug purity isn’t just a goal – it’s essential for achieving safety, stability and efficacy. However, purification is easier said than done, especially with challenging molecules like DNA and RNA “oligonucleotides,” due in large part to their diversity and the range of impurities that can be generated during production. Before you choose a chromatography resin for your next purification application, consider these five characteristics. Download the article to learn more.

Hamilton's PRP-Z2 stationary phase enables fast, robust separation of diquat and paraquat herbicides under EPA 549.2 and EPA 539 conditions.

From method concept to routine QC, analytical scientists must balance innovation with scalability, compliance, and reproducibility. This eBook explores how next-generation liquid chromatography (LC) systems are helping bridge that gap. Through three real-world examples you’ll see how modern LC platforms support confident decision-making across the entire analytical lifecycle.

This application note highlights a new multi-modal LC-MS/MS approach enabling robust analysis of polar and ionic metabolites without derivatization or ion-pairing.

Highlights amino acid analysis of rice and millets using a high-speed amino acid analyzer with a protein hydrolysate method, supporting nutritional characterization.

In this case study, an improvement in oligonucleotide peak shape is shown by suppressing the sample solvent effect through simultaneous injection with a desired solvent while using dedicated method development software to comprehensively evaluate co-injection solvent types and volumes to enable a more efficient workflow.

The article describes a comprehensive automated prep purification workflow targeting semaglutide, a GLP-1 receptor agonist, using dedicated method development software to automatically optimize a gradient profile and search for conditions that meet the specified separation criteria. This was followed by scale-up to purification scale and streamlined confirmation of the purities of collected fractions using benchtop MALDI-TOF MS.

This article describes how to efficiently achieve the optimal peak separation for charge variants of a monoclonal antibody using pH gradient ion-exchange chromatography and LabSolutions MD, dedicated software that can automate the entire method development workflow, including the generation of an analysis schedule, mobile phase preparation, and data processing.

This article describes the efficient development of robust analytical methods based on Analytical Quality by Design (AQbD) using dedicated LabSolutions MD software, which supports each phase of the process – screening, optimization, and validation – using functions such as experimental design, building of design space by automatic Peak Tracking, and robustness evaluation.

This article describes how dedicated software for supporting method development, in conjunction with single quad LCMS and inert UHPLC, can improve the overall method development workflow and efficiently achieve the optimal separation of oligonucleotides and related impurities.

This case study illustrates the streamlining of SFC method development using a mixture of six small molecule pharmaceuticals as a model sample, from automatic screening of columns and modifiers to design space evaluation for optimal conditions.

Since selenium is found in both food, water, and soil, its relative steady state can be a little challenging owing to its different oxidation states.

Identification of polyphenolic catechin compounds for health and industrial quantification and quality control of tea products.

Stay ahead with Picus® 2, the ergonomic electronic pipette designed for effortless, repeatable pipetting. Intuitive for all users yet packed with advanced features, Picus® 2 connects seamlessly to the Sartorius Pipetting mobile app, streamlining workflows, automating settings, and keeping your pipette updated. Smart, connected, and built for the future—because your lab deserves the best.

For sulfur compounds in the sub-ppmv to ppbv and lower concentrations, dynamically generated gas standards prevent the need for storing reactive and unstable components

This report shows how Hitachi’s Amino Acid Analyzer delivers high-speed, high-resolution analysis of HCl-hydrolyzed cat, dog, bird, and goldfish pet food samples

This eBook presents compelling examples of how the Agilent InfinityLab Pro iQ Plus mass detector can significantly elevate analysis and QC in biopharma.

The KNAUER Sepapure oliGO column is a good alternative to the commonly used competitor column for oligonucleotide analysis.

This technical note demonstrates a sensitive method for the quantitation of pasireotide in human plasma using high-resolution accurate mass spectrometry. A lower limit of quantitation (LLOQ) of 0.05 ng/mL was achieved in extracted plasma samples.

Explore best practices for LC-MS analysis of oligonucleotides to get the best results possible, quickly and easily.