Application Notes: General

As a leader responsible for metabolite identification in your organization, what keeps you awake at night? SCIEX can give you peace of mind by helping you . . .

Here we discuss a software-assisted drug metabolite identification and characterization workflow. The approach allows scientists to have increased confidence in the process.

The dream state If we lived in an ideal world, it would be possible to unambiguously identify metabolites using a single analytical experiment. This analytical technique would need to be efficient and easily generate the information needed from a routine assay that is also robust, enabling confident decision-making during drug discovery. At SCIEX, we believe that metabolite identification using the ZenoTOF 7600 system gets close to this dream state.

This application note presents the development and validation of a multi-component method for the analysis of 21 PFAS compounds in chicken eggs.

This application note presents the development and validation of a multiresidue method for the analysis of PFAS in baby food.

This application note presents the development and validation of a multiresidue method for the analysis of PFAS residues in beef, tuna, and shrimp.

This application note presents the development and validation of a multiresidue method for the analysis of PFAS in fruits, vegetables, and juices.

GRPC peptides are found in the entire body. Isolation of seven classic GRPC peptides initiating in the GI tract could implicate disease.

The diterpenoid, paclitaxel, which was identified as a potent chemotherapy agent for breast and ovarian cancer originates from the Pacific Yew tree. The isolation of paclitaxel from its major impurities is shown with the use of Hamilton’s PRP-1 (5 µm) HPLC column.

Plasticizers added to water bottles to add stability to the bottles can be unknowingly extracted in drinks leading to possible endocrine interactions.

The fast identification of tocopherols are important for various applications including free radical scavenging for human health and food stuff preservation.

Isolation of capsaicin for medicinal uses is easily achieved with the PRP-C18 RP-HPLC resin.

Discover how the HC-75 series uses a ligand exchange mechanism in order to interact more or less strongly with the analytes of interest.

Lee Ferguson, a Professor of Civil and Environmental Engineering at Duke University, and his group are developing and applying analytical methods to measure organic pollutants in the environment, with a focus on high-resolution mass spectrometry (HRMS) for detecting and quantifying emerging contaminants in wastewater and drinking water. In this video interview, we speak with Ferguson about his laboratory’s work accurately tracing these contaminants in complex environmental matrices.

This brief communitation hightlights the assessment of Hamilton PRP-3 HPLC column attributes when analyzing biologically active peptides.

Per- and polyfluoroalkyl substances demand precise detection. YMC-Triart C18 columns ensure superior separation and accurate quantification, even in complex matrices.

This application note presents an automated method for generating calibration curves using an autosampler’s dilution function. It offers practical examples demonstrating a simple setup, enhanced productivity, accuracy and reliability, with a focus on eliminating manual dilution for greater efficiency in HPLC analyses.

Part 2 delves into advanced applications using organic solvents with the HPLC autosampler dilution function. It details procedures for mixed standard solutions, highlighting automation’s role in reducing errors, saving labor and promoting efficient laboratory practices.

In BioTech R&D, overcoming data fragmentation is crucial as it hinders innovation, leads to redundant efforts, and wastes resources.

When high-quality, cost-effective impurity reference standards are unavailable, RRFs provide a practical alternative for impurity quantification.

Learn 10 tips for dynamically creating cylinder-free accurate calibration gas standards for ppmv and lower concentrations.

Molecular rotational resonance (MRR) spectroscopy operates in the microwave region, detecting transitions between pure rotational energy levels. Combined with adiabatic cooling, MRR produces highly selective spectra unique to each molecule’s atomic structure. Even slight changes in atomic position or mass result in distinct spectral differences, allowing MRR to directly analyze complex mixtures, including isomers, without chromatographic separation. This article presents a continuous headspace-MRR method for analyzing Class 2 residual solvents, including low-volatility solvents from Class 2 Mixture C. By evaluating solvents with diverse boiling points, the study demonstrates that MRR meets ICH and USP standards for most Class 2 and Class 3 solvents and half of Class 1 solvents.

Cyclic peptides, known for their stability and high specificity, are promising therapeutic agents in the fight against cancer, infections, and autoimmune diseases. However, developing effective cyclic peptides presents numerous challenges, including poor pharmacokinetics, efficacy, and toxicity. Traditional methods like liquid chromatography tandem-mass spectrometry (LC-MS/MS) often struggle with resolving isomeric linear peptide metabolites, posing significant risks in safety, efficacy, and regulatory approval. In this paper, Komal Kedia, PhD, will share how she leveraged MOBIE’s high-resolution ion mobility-mass spectrometry (IM-MS) system to achieve a 72% reduction in run times, 200% greater resolving power, and enhanced accuracy in identifying “soft spots” prone to enzymatic degradation.

The increasing demand for high-purity, regulatory-compliant guide RNA (gRNA) manufacturing in the CRISPR gene editing market underscores the need for advanced production capabilities.

This application demonstrates the LC-MS/MS analysis of 46 PFAS and 25 surrogate compounds in solid matrices using a newly developed, cost-effective workflow.