Application Notes: LC-MS

This application with data from Roche Diagnostics confirms superior performance of bioinert-coated YMC Accura BioPro IEX SF columns for the IEX-MS analysis of mAbs.

Discover the supremacy of the bioinert coated hardware of YMC Accura BioPro IEX QF columns for reproducible analysis of oligonucleotides in ion-exchange chromatography.

This application note provides you an optimum RP method for the DAR determination of a site-specific ADC using a widepore YMC-Triart Bio C4 column.

This SEC-MS method demonstrates the precise separation of a single-chain variable fragment from its drug-conjugate.

With this AEX method a mix of six oligonucleotides can be separated in under two minutes.

Experience high recoveries from the first injection! YMC Accura BioPro IEX SF columns provide excellent peak shapes for the ion-exchange analysis of mAbs.

In this study, the unique capabilities of a LC-UV/MS workflow to accelerate analysis are demonstrated in the identification of impurities related to GLP-1 analogs that underwent chemical and thermal stress conditions.

In this study, we present a QC-friendly LC-UV/MS workflow designed to alleviate the workload on analytical laboratories. Access to orthogonal mass data allows supporting labs to make quicker and more informed decisions during method development, reducing errors, and increasing overall productivity.

This method provides precise separation with sharp and symmetric peaks for the coordinating compounds of the kynurenine pathway.

An LC–MS/MS method for detecting and quantifying EpODEs and DiHODEs in rat plasma using oxylipin standards from α-linolenic acid (ALA) and γ-linolenic acid (GLA).

Achieve >99% spectral accuracy and sub-mDa precision on standard LC/MS systems. Learn how MassWorks™ enables confident formula ID—no high-resolution MS required.

Pfizer streamlined their LC/UV/MS data processing by adopting a semi-automated workflow with MS Workbook Suite, enabling faster, more consistent analysis and cutting process

Per- and polyfluoroalkyl substances demand precise detection. YMC-Triart C18 columns ensure superior separation and accurate quantification, even in complex matrices.

This application demonstrates the LC-MS/MS analysis of 46 PFAS and 25 surrogate compounds in solid matrices using a newly developed, cost-effective workflow.

A comparison made between a stainless steel hardware column and an INERT column using the same lot of HALO 90 Å C18, 2.7µm results in tailing factor and area improvement using the HALO®INERT column.

Using HALO®Elevate C18 and a high pH mobile phase obtains a great separation of drugs of abuse and metabolites without the use of a specialized stationary phase.

The positively charged surface chemistry of HALO® PCS Peptide delivers narrower peak width, higher signal response and double the peak capacities compared to standard uncharged peptide materials

Over the course of 5000 injections, the HALO®Elevate C18 1.5 mm ID column demonstrates stability and robustness in the stationary phase and the column hardware, with no significant changes to the retention time, peak shape, and back pressure.

A mix of four antidepressants is separated using the HALO®PCS (positive charged surface) C18 column demonstating the improved tailing factor and efficiency when compared to a traditional (uncharged) C18 stationary phase.

The HALO® OLIGO C18 2.7 µm column outperformed an FPP Oligo C18 column in oligonucleotide separations, achieving faster separations while maintaining efficiencies comparable to a 1.9 µm FPP with more than half the backpressure.

A separation of ultra-short and long chain PFAS (C1-C18) is performed on a HALO®PCS Phenyl-Hexyl column along with a HALO®PFAS Delay column which demonstrates excellent retention for both hydrophilic and hydrophobic analytes.

Crucial phosphorylated compounds can be analyzed by a polymeric iHILIC-(P) Classic HILIC column and MS. The method is applicable for targeted and non-targeted metabolomics.

This non-IP-RP method, using ammonium bicarbonate as mobile phase additive, is highly sensitive and suitable for the bioanalysis of therapeutic oligonucleotides.

The optimized LC-MS/MS method covering 388 signaling lipids shows that the bioinert coated YMC Accura Triart C18 column combines high sensitivity with high resolution.

In this application note, the charge variant characterisation of IgG4-based mAbs was performed using a BioPro IEX QF anion exchange column coupled to an MS detector.

The non-targeted screening of polar metabolites in human plasma with high sensitivity is achieved using a bioinert YMC Accura Triart Diol HILIC column.

The analysis of human plasma samples using a bioinert coated YMC Accura Triart C8 column demonstrates that real-life lipidomic analysis in under 10 minutes is possible.

This non-IP-RP method, using ammonium bicarbonate as mobile phase additive, is highly sensitive and suitable for the bioanalysis of therapeutic oligonucleotides.