Application Notes: LC-MS

LC–UV assay and organic impurities for paliperidone drug substance based on USP monograph where a L1 (C18) column with 100 x 4.6 mm, 3 µm dimensions was used

LC–MS/MS method to separate furosemide and its metabolite using three different columns: Kinetex 2.6 µm Biphenyl, Luna Omega 1.6 µm C18, and Luna Omega 1.6 µm Polar C18

Luna Omega 1.6µm, 3µm, and 5µm HPLC/UHPLC columns culminate 20 years of technological prowess, advancements, and innovation.

Easily determine the allowable adjustments that can be made to European Pharmacopoeia (EP) methods and find the right column for your method.

Quickly view allowable adjustments outlined by the United States Pharmacopeia (USP) and select the proper Phenomenex column for your monograph using this simple guide.

Analysis of ABN7 test mix used to assess scalability for Luna Omega C18 columns across a range of particle sizes (1.6 µm, 3 µm, and 5 µm) in three different dimensions.

Improve United States Pharmacopeia (USP) method performance with allowable adjustments

A robust and sensitive HPLC method with multi-wavelength UV–vis detector for the analysis of 2-phenoxyethanol and seven parabens in commercially available PCPs .

This application note demonstrates rapid peptide desalting prior to LC-MS analysis in Proteomics research using different micropipette SPE StageTips packed with CDS Analytical’s Empore SPE membranes.

Automated MHC-I is associated peptide enrichment using the Agilent AssayMAP Bravo platform with large-capacity Protein A cartridges for immunopeptidomics analysis.

The AssayMAP Bravo was used to purify, digest, and clean up antibodies from 96 cell culture supernatants with high reproducibility and minimal labor prior to LC–MS.

Intact and subunit antibody mass determination was streamlined using automated purification and enzymatic cleavage and deglycosylation of immobilized antibodies.

Automated sample prep and LC–MS peptide mapping approach to characterize and quantify multiple monoclonal antibody (mAb) critical quality attributes (CQAs)

Automated phosphopeptide enrichment from cell lysates with Fe(III)-NTA enables the identification of thousands phosphopepides with high reproducibly and selectivity.

Automated phosphopeptide enrichment and ion mobility mass spectrometry (IMS-MS) enables detailed analysis of structural effects of site-specific phosphorylation.

Automated Antibody Drug Conjugate (ADC) purification and deglycosylation increases reproducibility and simplifies drug-to-antibody ratio (DAR) determination

Improved host cell protein analysis using highly reproducible and scalable automated sample digestion and fractionation ahead of LC–MS coupled with iterative MS–MS

Easily implement a robust, reliable, and reproducible workflow solution for the analysis and quantitation of nine HAAs, bromate, and dalapon in water using IC–MS/MS.

This application note reveals the Vanquish Neo offers maximal MS utilization for direct injection workflows and versatility to separate peptides at low flow rates.

This application note shows a micro-flow LC–MS method developed on the Vanquish Neo UHPLC is suitable for high-throughput proteomic analyses of large sample cohorts.

ZipChip CE-MS offers a fast and easy method for native charge variant analysis and product monitoring workflows that are often challenging with traditional LC-MS.

Longer chain oligonucleotide therapeutics are more prevalent. The VN-50 2D column successfully separated 10-mer to 50-mer oligonucleotides without ion-pairing.

Phosphorothioated oligonucleotides were successfully separated with the VN-50 2D without ion-pairing and a simple acetonitrile/ammonium formate gradient.

The semi-micro VN-50 1D column contributed to lower diffusion and increased resolution and S/N ratio, necessary for highly sensitive QC analyses of oligonucleotide therapeutics.

The elution patterns of oligo-RNA having ribose modified with 2'-MOE and non-modified oligo-RNA were separated successfully without the use of an ion-pair reagent using the Shodex™ VN-50 2D column.

The analysis of FMOC-derivatized (fluorenmethyloxycarbonyl) glyphosate and AMPA according to DIN ISO 16308 is performed using a YMC-Triart C18 UHPLC column at pH 9.5.

This validation study demonstrates the use of the QSight® tandem quadrupole mass spectrometer (LC–MS/MS) for the application of PFAS EPA Method 537.1 in drinking water.

This application note details two LC–MS/MS methods for the analysis of 30 analytes from veterinary drugs in milk.